Allele Dmel\Met^W3

General Information
Symbol	Dmel\Met^W3	Species	D. melanogaster
Name		FlyBase ID	FBal0191692
Feature type	allele	Associated gene	Dmel\Met

Allele class
Mutagen	gamma ray
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	gamma ray (Wilson et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: E5G. (Wilson et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	chemical resistant (Wilson et al., 2006) visible (Baumann et al., 2010, Wilson et al., 2006)
Phenotype Manifest In
	embryonic/larval fat body \| wandering third instar larval stage, with Met^Scer\UAS.cBa, Scer\GAL4^Adh.PF (Liu et al., 2009) eye (Wilson et al., 2006) ommatidium (Baumann et al., 2010)
Detailed Description
	Statement Reference Mutants have defective facets in the posterior part of the eye. (Baumann et al., 2010) In addition to delayed larval development, the body weight of animals expressing Met[Scer\UAS.cBa] driven by Scer\GAL4[Adh.PF] in a hemizygous Met[W3] mutant background is dramatically reduced in comparison to Scer\GAL4[Adh.PF] controls. Once these mutants reach wandering third instar larval stage, their fat body cells begin to dissociate and undergo dramatic apoptosis. (Liu et al., 2009) Homozygotes show increased resistance to methoprene compared to controls. Homozygous females have a reduced rate of oviposition compared to controls. 78% of mutant flies have a small number of defective ommatidia in the posterior quarter of the eye. (Wilson et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Met^W3 has visible phenotype, enhanceable by br^rbp-2 (Wilson et al., 2006) Met^W3 has visible phenotype, enhanceable by gce^{dsRNA.Scer\UAS.cBa}/Scer\GAL4^Act5C.PI (Baumann et al., 2010)
NOT Enhanced by
Statement Reference Met^W3 has visible phenotype, non-enhanceable by br¹ (Wilson et al., 2006)
Suppressed by
Statement Reference Met^W3 has visible phenotype, suppressible by gce^Scer\UAS.cBa/Scer\GAL4^dan-AC116 (Baumann et al., 2010) Met^W3 has visible phenotype, suppressible by gce^Scer\UAS.cBa/Scer\GAL4^tub.PU (Baumann et al., 2010)
NOT suppressed by
Statement Reference Met^W3 has visible phenotype, non-suppressible by gce^Scer\UAS.cBa/Scer\GAL4^Lsp2.PH (Baumann et al., 2010)
Other
Statement Reference Met^W3, br¹ has lethal \| pharate adult stage phenotype (Wilson et al., 2006, Wilson et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference Met^W3 has eye phenotype, enhanceable by br^rbp-2 (Wilson et al., 2006) Met^W3 has ommatidium phenotype, enhanceable by gce^{dsRNA.Scer\UAS.cBa}/Scer\GAL4^Act5C.PI (Baumann et al., 2010)
NOT Enhanced by
Statement Reference Met^W3 has eye phenotype, non-enhanceable by br¹ (Wilson et al., 2006)
Suppressed by
Statement Reference Met^W3 has ommatidium phenotype, suppressible by gce^Scer\UAS.cBa/Scer\GAL4^dan-AC116 (Baumann et al., 2010) Met^W3 has ommatidium phenotype, suppressible by gce^Scer\UAS.cBa/Scer\GAL4^tub.PU (Baumann et al., 2010)
NOT suppressed by
Statement Reference Met^W3 has ommatidium phenotype, non-suppressible by gce^Scer\UAS.cBa/Scer\GAL4^Lsp2.PH (Baumann et al., 2010)
Enhancer of
Statement Reference Met^W3/Met^W3 is an enhancer of ovary phenotype of br¹ (Wilson et al., 2006, Wilson et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The defective facet phenotype seen in Met[W3] flies is suppressed by expression of gce[Scer\UAS.cBa] under the control of either Scer\GAL4[tub.PU] or Scer\GAL4[dan-AC116], but is not suppressed by expression of gce[Scer\UAS.cBa] under the control of Scer\GAL4[Lsp2.PH]. Expression of gce[dsRNA.Scer\UAS.cBa] under the control of Scer\GAL4[Act5C.PI] enhances the defective facet phenotype seen in Met[W3] flies. (Baumann et al., 2010) The expressivity of the defective eye phenotype seen in Met[W3] flies is increased by br[rbp-2] but not by br[1]. (Wilson et al., 2006) br¹, Rst(1)JH^W3 double mutant escapers show significantly reduced levels of oogenesis compared to br¹ single mutants. (Wilson et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Met^W3 is rescued by Scer\GAL4[-]/Met^Scer\UAS.cBa (Barry et al., 2008) Met^W3 is rescued by Scer\GAL4^Act.PU/Met^Scer\UAS.cBa (Barry et al., 2008)
Comments	Either of the transgenic lines Met[hs.PA] or P{UAS-Met.B}pV2 can rescue the Met[W3] mutant eye phenotype due to their Scer\GAL4-independent expression of Met[Scer\UAS.cBa]. The methoprene resistance of Met[W3] mutants is also lost as a result of Met[Scer\UAS.cBa] expression. Scer\GAL4[Act]-driven Met[Scer\UAS.cBa] rescues the Met[W3] mutant eye phenotype. (Barry et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Met^W3 (Wilson et al., 2006, Wilson et al., 2006, Baumann et al., 2010, Barry et al., 2008) Met^w3 (Liu et al., 2009) Rst(1)JH^W3
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Baumann et al., 2010, Genetics 185(4): 1327--1336 Paralogous Genes Involved in Juvenile Hormone Action in Drosophila melanogaster. [FBrf0211496] Liu et al., 2009, Development 136(12): 2015--2025 Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila. [FBrf0207947] Barry et al., 2008, Insect Biochem. Mol. Biol. 38(3): 346--353 Overexpression of Methoprene-tolerant, a Drosophila melanogaster gene that is critical for juvenile hormone action and insecticide resistance. [FBrf0202716] Wilson et al., 2006, Genetics 172(1): 253--264 Interaction between hormonal signaling pathways in Drosophila melanogaster as revealed by genetic interaction between Methoprene-tolerant and Broad-Complex. [FBrf0190778] Wilson et al., 2006, Mol. Genet. Genomics 276(3): 294--303 Wide mutational spectrum of a gene involved in hormone action and insecticide resistance in Drosophila melanogaster. [FBrf0193291]

Allele Dmel\MetW3

Allele Dmel\Met^W3