The piggyBac PBac{RB}ppk25e04217 is inserted into exon 2 of ppk25 making it unlikely that the this intron can be spliced properly.
ppk25e04217/Df(2R)Δ42E mutant males do not display courtship behavior during a 10 minute observation period under infrared light. ppk25e04217 heterozygous males exhibit significantly reduced levels of courtship behavior compared to males that carry only one copy of wild-type ppk25 (in Df(2R)Δ42E flies), indicating ppk25e04217 to be a dominant-negative mutation. Under infrared light ppk25e04217/Df(2R)Δ5-22 mutants exhibit a complete loss of male response to females, but no decrease in walking or preening. In visible light, these males perform all the normal steps of courtship, albeit to significantly reduced levels. In addition, the average length of a courtship bout for mutant males is less than half that of controls, suggesting that ppk25e04217 affects a subsequent step in courtship.
