FlyBase Allele Report: Dmel\mib1[UAS.Tag:polyHis,Tag:MYC]

General Information

Symbol

Dmel\mib1^{UAS.Tag:polyHis,Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0191877

Feature type

allele

Associated gene

Dmel\mib1

Associated Insertion(s)

Carried in Construct

P{UAS-mib1.His6.MYC}

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - coding region fusion

(Lai et al., 2005)

in vitro construct - regulatory fusion

(Lai et al., 2005)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-mib1.His6.MYC}

Cytology

Nature of the lesion

Statement

Reference

UAS sequences drive expression of full-length mib1, tagged Tag:polyHis and 3 Tag:MYC tags.

(Lai et al., 2005)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

crossvein, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

head bristle, with Scer\GAL4^sca-537.4

(Lai et al., 2005)

sensillum campaniformium, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

sensory mother cell, with Scer\GAL4^sca-537.4

(Lai et al., 2005)

wing vein, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing vein L4, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Lai et al., 2005)

Detailed Description

Statement

Reference

Expression of mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}, under the control of Scer\GAL4^sca-537.4, induces a mild loss of macrochaete bristles from the head and loss of some L3 sensory precursors from the third instar wing disc. Expression of this transgene under the control of Scer\GAL4^Bx-MS1096 leads to longitudinal vein breaks in the wing and missing crossveins. The wings of flies expressing mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}, under the control of Scer\GAL4^dpp.blk1, lack campaniform sensilla and show a mild loss of wing veins. However, these flies show normal wing margin development.

(Lai et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a non-enhancer of wing disc | ventral phenotype of Scer\GAL4^dpp.blk1, Ser^UAS.cSa

(Lai et al., 2005)

Suppressor of

Statement

Reference

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a suppressor of sensillum campaniformium phenotype of Scer\GAL4^dpp.blk1, neur^αRING.UAS

(Lai et al., 2005)

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a suppressor of wing vein L3 phenotype of Scer\GAL4^dpp.blk1, neur^αRING.UAS

(Lai et al., 2005)

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a suppressor of wing margin phenotype of Scer\GAL4^dpp.blk1, neur^αRING.UAS

(Lai et al., 2005)

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^sca-537.4 is a suppressor of external sensory organ precursor cell IIa phenotype of neur¹¹

(Lai et al., 2005)

NOT Suppressor of

Statement

Reference

mib1^{UAS.Tag:polyHis,Tag:MYC}/Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc | ventral phenotype of Scer\GAL4^dpp.blk1, Ser^UAS.cSa

(Lai et al., 2005)

Other

Statement

Reference

Dl^UAS.cDa, Scer\GAL4^dpp.blk1, mib1^{UAS.Tag:polyHis,Tag:MYC} has wing disc | ventral phenotype

(Lai et al., 2005)

Scer\GAL4^sca-537.4, mib1^{UAS.Tag:polyHis,Tag:MYC}, neur¹¹ has macrochaeta & mesothoracic tergum | somatic clone phenotype

(Lai et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-misexpression of mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} with Dl^Scer\UAS.cDa along the anteroposterior compartment boundary of the wing disc, under the control of Scer\GAL4^dpp.blk1, results in increased ventral disc overgrowth and ectopic wing margins that span the ventral compartment. The additional expression of mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} in wing discs expressing Ser^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 does not change the wing disc phenotype. Coexpression of mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} rescues the wing phenotype seen when neur^{αRING.Scer\UAS} is expressed under the control of Scer\GAL4^dpp.blk1. Expression of neur^Scer\UAS.cLa partially rescues the wing phenotype of flies that express mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1. This phenotype is almost completely suppressed when both mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} and two copies of neur^Scer\UAS.cLa are expressed under the control of Scer\GAL4^dpp.blk1 alongside mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}. Expression of mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^sca-537.4 rescues bald neur¹¹ clones back to a mild bristle tufting phenotype. Staining at the sensory precursor level shows that mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} expression rescues pIIa specification in neur¹¹ clones.

(Lai et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}

mib1^{UAS.Tag:polyHis,Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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