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General Information
Symbol
Dmel\DarkL46
Species
D. melanogaster
Name
FlyBase ID
FBal0191880
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
arkL46
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G17024768A

Amino acid change:

W950term | Dark-PB

Reported amino acid change:

W950term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase base on reported amino acid change.

point mutation
Nucleotide change:

T17022612G

Amino acid change:

C346W | Dark-PA; C346W | Dark-PB

Reported amino acid change:

C346W

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: C346W.

Amino acid replacement: W950term.

Amino acid replacement: C346T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Salivary glands are degraded by 24 hours after puparium formation (as occurs in wild-type controls) in ArkN28/ArkL46 mutants that develop normally.

ArkL46/ArkP46 flies exhibit melanotic tumors and abnormal wings.

ArkL46 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

Homozygous clones in the eye result in an excess of interommatidial cells in the retina at 42 hours after puparium formation compared to wild type. Mutant clones at the edge of the eye have extra perimeter ommatidial cells that are never eliminated.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

DarkL46 is a suppressor | partially of eye phenotype of hidGMR.PG/WGMR.PG

Additional Comments
Genetic Interactions
Statement
Reference

The WGMR.PG eye ablation phenotype is strongly suppressed when eyes carry homozygous clones for ArkL46.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)