Allele Dmel\tkv^xtr

General Information
Symbol	Dmel\tkv^xtr	Species	D. melanogaster
Name	extruded	FlyBase ID	FBal0191914
Feature type	allele	Associated gene	Dmel\tkv

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Gibson and Perrimon, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Gibson and Perrimon, 2005)
Phenotype Manifest In
	dorsal mesothoracic disc & actin filament \| somatic clone (Gibson and Perrimon, 2005) dorsal mesothoracic disc & microtubule \| apical \| somatic clone (Gibson and Perrimon, 2005) leg & adult cuticle \| somatic clone (Gibson and Perrimon, 2005) wing & adult cuticle \| somatic clone (Gibson and Perrimon, 2005) wing disc \| somatic clone (Gibson and Perrimon, 2005)
Detailed Description
	Statement Reference Homozygous clones in medial regions of the wing disc epithelium show defects in their ability to establish or maintain the pseudostratified columnar cell shape, resulting in their retraction from the apical epithelial surface and subsequent basal extrusion as inverted epithelial cysts. Fewer mutant clones are recovered compared to their twin spot controls in the presumptive medial blade territory, suggesting that some mutant clones undergo cell death. A large number of extruding clones are recovered in the presumptive hinge and notum regions, with a fraction of these extending apically rather than basally. Apical microtubule arrays are eliminated cell-autonomously in homozygous clones in the wing disc, while basal microtubule networks are unaffected. The cells also show a subtle but consistent reduction of F-actin at the basolateral cortex of cells on the clone interior, transient ectopic accumulation of F-actin at the apical adherens junctions and ectopic accumulation of F-actin at boundaries between mutant cells and their wild-type neighbours. Many homozygous clones survive metamorphosis, forming differentiated inverted cuticular vesicles lodged between the dorsal and ventral surfaces of the adult wing or leg. (Gibson and Perrimon, 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement Reference tkv^xtr has wing disc \| somatic clone phenotype, non-suppressible by Scer\GAL4^T155/BacA\p35^Scer\UAS.cHa (Gibson and Perrimon, 2005)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^T155 does not rescue the extrusion of tkv^xtr clones from the wing disc. (Gibson and Perrimon, 2005)
Complementation & Rescue Data
Fails to complement	tkv⁴ (Gibson and Perrimon, 2005) tkv⁷ (Gibson and Perrimon, 2005) tkv^K16159 (Gibson and Perrimon, 2005)
Rescued by	tkv^xtr is rescued by tkv^Scer\UAS.cNa/Scer\GAL4^tub.PU (Gibson and Perrimon, 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	EMS^4a21 (Gibson and Perrimon, 2005) tkv^xtr (Szuperák et al., 2011)
Name Synonym	extruded (Gibson and Perrimon, 2005)
Secondary FlyBase IDs

References ( 2 )
Research paper	Szuperák et al., 2011, Development 138(4): 715--724 Feedback regulation of Drosophila BMP signaling by the novel extracellular protein Larval Translucida. [FBrf0212881] Gibson and Perrimon, 2005, Science 307(5716): 1785--1789 Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing. [FBrf0184250]

Allele Dmel\tkvxtr

Allele Dmel\tkv^xtr