Nmdar1EP3511 mutants exhibit a significant reduction of acquired tolerance to ethanol vapor-induced sedation in chronic tolerance assays, and they show a trend towards reduced tolerance in acute tolerance assays, as compared to controls.
Mutant flies show defects in learning (memory measured immediately after conditioning) in an aversive olfactory conditioning assay. Short-term memory (assayed 1 hour after training) is also defective compared to controls. One-day memory after spaced training (long-term memory) is reduced compared to wild type, while one-day memory after massed training (anesthesia-resistant memory) is normal.
Nmdar1EP331 mutants exhibit reduced short-term and long-term memory. Extinction learning appears to be normal in these mutants.
Disruption of Nmdar1 through expression of Nmdar1EP331 under the control of Scer\GAL4hs.PB does not result in a significant decrease in PI (preference index - with respect to light) in constant darkness conditions compared with control flies (indicating that phototaxis preference behaviour plasticity is inhibited by down-regulation of Nmdar1 during the critical period).
Nmdar1EP331/Scer\GAL4hs.PB flies reared in a normal light/dark cycle for 4 days at 18[o]C that are then treated with heat-shock and transferred to constant darkness exhibit no significant difference in PI (preference index - with respect to light) in constant darkness conditions compared with control flies.
Olfactory learning after Pavlovian conditioning is reduced in Nmdar1EP331 mutants compared to wild-type flies. However, sensorimotor responses to odors and foot shock stimuli are not affected. Expression of one copy of Nmdar1EP331 under the control of one copy of Scer\GAL4P26.hs, following heat shock, yields an antisense transcript of Nmdar1. This acute repression of Nmdar1 leads to a transient, severe disruption of olfactory learning. This acute repression also disrupts one day training after spaced training, while one day memory after massed training is normal, showing that long term memory is defective, but anesthesia-resistant memory is normal. Sensorimotor responses to odors and foot shock stimuli are not affected.