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General Information
Symbol
Dmel\Nmdar1EP331
Species
D. melanogaster
Name
FlyBase ID
FBal0191934
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{EP} insertion 425bp downstream of the 3' end of the Nmdar1 transcription unit.

Insertion components
P{EP}Nmdar1EP331
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nmdar1EP3511 mutants exhibit a significant reduction of acquired tolerance to ethanol vapor-induced sedation in chronic tolerance assays, and they show a trend towards reduced tolerance in acute tolerance assays, as compared to controls.

Mutant flies show defects in learning (memory measured immediately after conditioning) in an aversive olfactory conditioning assay. Short-term memory (assayed 1 hour after training) is also defective compared to controls. One-day memory after spaced training (long-term memory) is reduced compared to wild type, while one-day memory after massed training (anesthesia-resistant memory) is normal.

Nmdar1EP331 mutants exhibit reduced short-term and long-term memory. Extinction learning appears to be normal in these mutants.

Disruption of Nmdar1 through expression of Nmdar1EP331 under the control of Scer\GAL4hs.PB does not result in a significant decrease in PI (preference index - with respect to light) in constant darkness conditions compared with control flies (indicating that phototaxis preference behaviour plasticity is inhibited by down-regulation of Nmdar1 during the critical period).

Nmdar1EP331/Scer\GAL4hs.PB flies reared in a normal light/dark cycle for 4 days at 18[o]C that are then treated with heat-shock and transferred to constant darkness exhibit no significant difference in PI (preference index - with respect to light) in constant darkness conditions compared with control flies.

Olfactory learning after Pavlovian conditioning is reduced in Nmdar1EP331 mutants compared to wild-type flies. However, sensorimotor responses to odors and foot shock stimuli are not affected. Expression of one copy of Nmdar1EP331 under the control of one copy of Scer\GAL4P26.hs, following heat shock, yields an antisense transcript of Nmdar1. This acute repression of Nmdar1 leads to a transient, severe disruption of olfactory learning. This acute repression also disrupts one day training after spaced training, while one day memory after massed training is normal, showing that long term memory is defective, but anesthesia-resistant memory is normal. Sensorimotor responses to odors and foot shock stimuli are not affected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Nmdar1EP331 has memory defective phenotype, enhanceable by rut[+]/rut1

Enhancer of
Statement
Reference

Nmdar1EP331 is an enhancer of memory defective phenotype of rut1

Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The climbing defects of mir-1000KO1/mir-1000KO2 adults are rescued if they are also carrying Nmdar1EP331/+, and lifespan is increased (although not to control levels).

rut1/+; Nmdar1EP331/Nmdar1EP331 double mutants exhibit significantly lower performance in standard Pavlovian learning assays compared to either single mutant.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by

Nmdar1EP331 is not rescued by Nmdar1tCosmidA/Scer\GAL4[-]

Comments

Expression of either Nmdar1Scer\UAS.cMa or Nmdar1N631Q.Scer\UAS under the control of Scer\GAL4elav.PU rescues the learning defects seen in Nmdar1EP331 flies.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (8)