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General Information
Symbol
Dmel\aPKCCAAXWT.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0192028
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-aPKCCAAX, aPKCCAAX, UAS-aPKCCAAXWT, UAS-aPKC-CAAX
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a membrane-targeted (by the CAAX motif) wild-type aPKC sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4Bx-MS1096 does not lead to obvious size defects in third instar larval wing discs, as compared to controls.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4da.G32 does not affect epithelial cell polarity.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4erm.R9D11 results in supernumerary type II neuroblasts in the larval brain.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA results in supernumerary type I neuroblasts in the larval brain.

Expression of aPKCCAAXWT.Scer\UAS in amnioserosa under the control of Scer\GAL4332.3 has no significant phenotypic effect on actomyosin assembly-disassembly cycles.

Expression of aPKCCAAXWT.Scer\UAS in adult Malpighian tubule clones (using the MARCM system, under the control of Scer\GAL80αTub84B.PL and Scer\GAL4Scer\FRT.Act5C) blocks renal and nephric stem cell (RNSC) proliferation and differentiation.

Expression of aPKCCAAXWT.Scer\UAS in differentiated retinal cells in the eye under the control of Scer\GAL4GMR.PF results in severe defects in the apicobasal polarity of the retinal cells, generating a small, rough eye phenotype.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 29[o]C results in strong overproliferation of neuroblasts at the expense of neurons in the larval brain.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C results in modest overproliferation of neuroblasts at the expense of neurons in the larval brain.

Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA results in a large increase in the number of neuroblasts in the larval brain.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of aPKCCAAXWT.Scer\UAS with hepScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 leads to moderate overgrowth and cell polarity defects in third instar larval wing discs, whereas co-expression with either ph-pGD4480 or par-6Scer\UAS.cKa leads to a severe overgrowth, as compared to controls.

Expression of aPKCCAAXWT.Scer\UAS in a homozygous yrt75a background results in a striking apicalisation phenotype, characterised by extreme extension of the apical membrane of epidermal cells leading to the formation of inverted cysts toward the end of embryogenesis. Consequently, the larval cuticle, secreted through the apical domain, forms little spheres typical of apicalised epidermal cells.

The formation of supernumerary type I neuroblasts which is seen the brains of larvae expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA is suppressed by co-expression of numbΔC.Scer\UAS.T:Ivir\HA1, numbScer\UAS.T:Ivir\HA1, numbS2A.Scer\UAS.T:Ivir\HA1 or numbS2D.Scer\UAS.T:Ivir\HA1, but is not suppressed by co-expression of numbΔAB.Scer\UAS.T:Ivir\HA1 or numbΔPTB.Scer\UAS.T:Ivir\HA1.

Co-expression of par-6Scer\UAS.cKa and aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4332.3 significantly reduces the network pulse frequency for actomyosin assembly-disassembly cycles. Lull times are also significantly higher than in wild-type, and have wide variation. However, pulse durations are statistically indistinguishable from those of controls. Some cells with par-6Scer\UAS.cKa plus aPKCCAAXWT.Scer\UAS overexpression never form actomyosin networks.

Expression of aPKCCAAXWT.Scer\UAS in differentiated retinal cells in the eye under the control of Scer\GAL4GMR.PF in a mts02496 or mtsXE-2258 mutant background results in a smaller and rougher eye than in a wild-type background.

The overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 29[o]C is suppressed by co-expression of numbfl.Scer\UAS.T:Hsap\MYC.

The modest overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C is enhanced by numb15/+ and weakly suppressed by numbS52F/+.

Co-expression of l(2)gl3A.Scer\UAS strongly suppresses the increased number of neuroblasts in the larval brain caused by expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
aPKCCAAXWT.Scer\UAS
aPKCCAAXWT.UAS
Name Synonyms
Secondary FlyBase IDs
    References (23)