FB2025_01 , released February 20, 2025
Allele: Dmel\aPKCUAS.Tag:CAAX(Unk)
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General Information
Symbol
Dmel\aPKCUAS.Tag:CAAX(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0192028
Feature type
allele
Associated gene
Dmel\aPKC
Associated Insertion(s)
Carried in Construct
P{UAS-aPKC.WT.CAAX}
Also Known As
UAS-aPKCCAAX, UAS-aPKC-CAAX, aPKCCAAX, UAS-aPKCCAAXWT, UAS-DaPKCCAAXWT, UAS-aPKC[CAAX]
Key Links
Transgenic product class
wild_type
(Sotillos et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sotillos et al., 2004)
Mutagen
in vitro construct
(Lee et al., 2006, Sotillos et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-aPKC.WT.CAAX}
Cytology
Description

UASt regulatory sequences drive expression of wild-type aPKC that is targeted to the membrane due to the presence of a Tag:CAAX(Unk) tag.

(Sotillos et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
aPKC
Tagged with
Tag:CAAX(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA with hepUAS.cBa
      (Bunker et al., 2015)
      CEA with ph-pGD4480
      (Bunker et al., 2015)
      model of  high grade glioma
      CEA
      (Paglia et al., 2017)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  high grade glioma
      is ameliorated by MycRNAi.UAS.cUa
      (Paglia et al., 2017)
      is exacerbated by Pi3K92ECAAX.UAS
      (Paglia et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4Bx-MS1096 does not lead to obvious size defects in third instar larval wing discs, as compared to controls.

      (Bunker et al., 2015)

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4da.G32 does not affect epithelial cell polarity.

      (Gamblin et al., 2014)

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4erm.R9D11 results in supernumerary type II neuroblasts in the larval brain.

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA results in supernumerary type I neuroblasts in the larval brain.

      (Haenfler et al., 2012)

      Expression of aPKCCAAXWT.Scer\UAS in amnioserosa under the control of Scer\GAL4332.3 has no significant phenotypic effect on actomyosin assembly-disassembly cycles.

      (David et al., 2010)

      Expression of aPKCCAAXWT.Scer\UAS in adult Malpighian tubule clones (using the MARCM system, under the control of Scer\GAL80αTub84B.PL and Scer\GAL4Scer\FRT.Act5C) blocks renal and nephric stem cell (RNSC) proliferation and differentiation.

      (Zeng et al., 2010)

      Expression of aPKCCAAXWT.Scer\UAS in differentiated retinal cells in the eye under the control of Scer\GAL4GMR.PF results in severe defects in the apicobasal polarity of the retinal cells, generating a small, rough eye phenotype.

      (Ogawa et al., 2009)

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 29[o]C results in strong overproliferation of neuroblasts at the expense of neurons in the larval brain.

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C results in modest overproliferation of neuroblasts at the expense of neurons in the larval brain.

      (Wirtz-Peitz et al., 2008)

      Expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA results in a large increase in the number of neuroblasts in the larval brain.

      (Lee et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk) has type I neuroblast | increased number | larval stage phenotype, suppressible by numbUAS.Tag:HA/Scer\GAL4wor.PA

      (Haenfler et al., 2012)

      Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk) has type I neuroblast | increased number | larval stage phenotype, suppressible by Scer\GAL4wor.PA/numbS2A.UAS.Tag:HA

      (Haenfler et al., 2012)

      Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk) has type I neuroblast | increased number | larval stage phenotype, suppressible by numbS2D.UAS.Tag:HA/Scer\GAL4wor.PA

      (Haenfler et al., 2012)

      Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk) has type I neuroblast | increased number | larval stage phenotype, suppressible by Scer\GAL4wor.PA/numbΔC.UAS.Tag:HA

      (Haenfler et al., 2012)

      Scer\GAL4insc-Mz1407, aPKCUAS.Tag:CAAX(Unk) has larval neuroblast phenotype, suppressible | partially by numb[+]/numbS52F

      (Wirtz-Peitz et al., 2008)

      Scer\GAL4insc-Mz1407, aPKCUAS.Tag:CAAX(Unk) has larval brain | larval stage phenotype, suppressible | partially by numb[+]/numbS52F

      (Wirtz-Peitz et al., 2008)

      Scer\GAL4insc-Mz1407, aPKCUAS.Tag:CAAX(Unk) has larval neuroblast phenotype, suppressible by Scer\GAL4insc-Mz1407/numbfl.UAS.Tag:MYC

      (Wirtz-Peitz et al., 2008)

      Scer\GAL4insc-Mz1407, aPKCUAS.Tag:CAAX(Unk) has larval brain | larval stage phenotype, suppressible by Scer\GAL4insc-Mz1407/numbfl.UAS.Tag:MYC

      (Wirtz-Peitz et al., 2008)

      Scer\GAL4wor.PA, aPKCUAS.Tag:CAAX(Unk) has larval brain & neuroblast | supernumerary phenotype, suppressible by l(2)gl3A.UAS/Scer\GAL4wor.PA

      (Lee et al., 2006)
      NOT suppressed by
      NOT Suppressor of
      Statement
      Reference

      aPKCUAS.Tag:CAAX(Unk)/Scer\GAL4sns.PK is a non-suppressor of garland cell phenotype of Scer\GAL4sns.PK, bazRNAi.UAS

      (Heiden et al., 2021)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of aPKCCAAXWT.Scer\UAS with hepScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 leads to moderate overgrowth and cell polarity defects in third instar larval wing discs, whereas co-expression with either ph-pGD4480 or par-6Scer\UAS.cKa leads to a severe overgrowth, as compared to controls.

      (Bunker et al., 2015)

      Expression of aPKCCAAXWT.Scer\UAS in a homozygous yrt75a background results in a striking apicalisation phenotype, characterised by extreme extension of the apical membrane of epidermal cells leading to the formation of inverted cysts toward the end of embryogenesis. Consequently, the larval cuticle, secreted through the apical domain, forms little spheres typical of apicalised epidermal cells.

      (Gamblin et al., 2014)

      The formation of supernumerary type I neuroblasts which is seen the brains of larvae expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA is suppressed by co-expression of numbΔC.Scer\UAS.T:Ivir\HA1, numbScer\UAS.T:Ivir\HA1, numbS2A.Scer\UAS.T:Ivir\HA1 or numbS2D.Scer\UAS.T:Ivir\HA1, but is not suppressed by co-expression of numbΔAB.Scer\UAS.T:Ivir\HA1 or numbΔPTB.Scer\UAS.T:Ivir\HA1.

      (Haenfler et al., 2012)

      Co-expression of par-6Scer\UAS.cKa and aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4332.3 significantly reduces the network pulse frequency for actomyosin assembly-disassembly cycles. Lull times are also significantly higher than in wild-type, and have wide variation. However, pulse durations are statistically indistinguishable from those of controls. Some cells with par-6Scer\UAS.cKa plus aPKCCAAXWT.Scer\UAS overexpression never form actomyosin networks.

      (David et al., 2010)

      Expression of aPKCCAAXWT.Scer\UAS in differentiated retinal cells in the eye under the control of Scer\GAL4GMR.PF in a mts02496 or mtsXE-2258 mutant background results in a smaller and rougher eye than in a wild-type background.

      (Ogawa et al., 2009)

      The overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 29[o]C is suppressed by co-expression of numbfl.Scer\UAS.T:Hsap\MYC.

      The modest overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C is enhanced by numb15/+ and weakly suppressed by numbS52F/+.

      (Wirtz-Peitz et al., 2008)

      Co-expression of l(2)gl3A.Scer\UAS strongly suppresses the increased number of neuroblasts in the larval brain caused by expression of aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA.

      (Lee et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      aPKCCAAX
      (Wirtz-Peitz et al., 2008)
      aPKCCAAXWT.Scer\UAS
      aPKCCAAXWT.UAS
      aPKCUAS.Tag:CAAX(Unk)
      Name Synonyms
      Secondary FlyBase IDs
        References (34)