The co-expression of aPKCCAAXWT.Scer\UAS with hepScer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 leads to moderate overgrowth and cell polarity defects in third instar larval wing discs, whereas co-expression with either ph-pGD4480 or par-6Scer\UAS.cKa leads to a severe overgrowth, as compared to controls.
Expression of aPKCCAAXWT.Scer\UAS in a homozygous yrt75a background results in a striking apicalisation phenotype, characterised by extreme extension of the apical membrane of epidermal cells leading to the formation of inverted cysts toward the end of embryogenesis. Consequently, the larval cuticle, secreted through the apical domain, forms little spheres typical of apicalised epidermal cells.
The formation of supernumerary type I neuroblasts which is seen the brains of larvae expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4wor.PA is suppressed by co-expression of numbΔC.Scer\UAS.T:Ivir\HA1, numbScer\UAS.T:Ivir\HA1, numbS2A.Scer\UAS.T:Ivir\HA1 or numbS2D.Scer\UAS.T:Ivir\HA1, but is not suppressed by co-expression of numbΔAB.Scer\UAS.T:Ivir\HA1 or numbΔPTB.Scer\UAS.T:Ivir\HA1.
Co-expression of par-6Scer\UAS.cKa and aPKCCAAXWT.Scer\UAS under the control of Scer\GAL4332.3 significantly reduces the network pulse frequency for actomyosin assembly-disassembly cycles. Lull times are also significantly higher than in wild-type, and have wide variation. However, pulse durations are statistically indistinguishable from those of controls. Some cells with par-6Scer\UAS.cKa plus aPKCCAAXWT.Scer\UAS overexpression never form actomyosin networks.
Expression of aPKCCAAXWT.Scer\UAS in differentiated retinal cells in the eye under the control of Scer\GAL4GMR.PF in a mts02496 or mtsXE-2258 mutant background results in a smaller and rougher eye than in a wild-type background.
The overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 29[o]C is suppressed by co-expression of numbfl.Scer\UAS.T:Hsap\MYC.
The modest overproliferation of neuroblasts seen in the larval brain in animals expressing aPKCCAAXWT.Scer\UAS under the control of Scer\GAL41407 at 18[o]C is enhanced by numb15/+ and weakly suppressed by numbS52F/+.