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General Information
Symbol
Dmel\psqGS88A8
Species
D. melanogaster
Name
FlyBase ID
FBal0192088
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GS88A8, eyeful
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion upstream of psq, in an intron of lola. The insertion causes overexpression of both lola and psq in the presence of a Scer\GAL4 driver.
    Insertion components
    P{GSV1}GS88A8
    Product class / Tool use(s)
    Encoded product / tool
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    Other
    Phenotype Manifest In
    Enhanced by
    NOT Enhanced by
    Suppressed by
    Statement
    Reference
    DlUAS.cDa, Scer\GAL4ey.PH, lolaGS88A8, psqGS88A8 has eye phenotype, suppressible by Su(var)3-9[+]/Su(var)3-917
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Co-expression of DlScer\UAS.cDa with lola and psq (using P{GSV1}lolaGS88A8, which is comprised of the lolaGS88A8 and psqGS88A8 alleles) under the control of Scer\GAL4ey.PU induces tumor-like overgrowths in the eye. The tumor-like overgrowth phenotype seen when DlScer\UAS.cDa is co-expressed P{GSV1}lolaGS88A8 (comprised of the lolaGS88A8 and psqGS88A8) under the control of Scer\GAL4ey.PU is seen with increased frequency when one copy of mirrDFM3 is also present. The tumor-like overgrowth phenotype seen when DlScer\UAS.cDa is co-expressed P{GSV1}lolaGS88A8 (comprised of the lolaGS88A8 and psqGS88A8) under the control of Scer\GAL4ey.PU is seen with increased frequency upon co-expression of mirrGD16318.
    Co-expression of DlScer\UAS.cDa, lolaGS88A8 and psqGS88A8 using Scer\GAL4ey.PH (the so-called 'eyeful' background) results in a delay in pupariation of 26.6 +/- 7.5 hours. This is reverted to a delay of 5.9 +/- 7.1 hours in a Ilp8MI00727 background.
    72.5% of flies expressing DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 under the control of Scer\GAL4ey.PU have eye tumours, with 4.9% having macroscopically visible secondary tumour growths derived from the developing retina. The incidence of primary and secondary tumours in these "eyeful" flies is slightly enhanced by co-expression of ctdsRNA.cGa.Scer\UAS, but is greatly enhanced if the "eyeful" flies also co-express both ctdsRNA.cGa.Scer\UAS and BacA\p35Scer\UAS.cUa.
    Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which causes overexpression of psq and lola) show an eye tumour phenotype. In addition, distant metastasis in the thorax and abdomen is seen in 15% of cases. Co-expression of mir-8SC1 significantly reduces the primary eye tumour size, completely inhibits the incidence of metastases and results in a high incidence of adult lethality.
    Scer\GAL4ey.PH-mediated expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 causes mild overgrowth of the eye (the 'eyeful' phenotype) at 18[o]C.
    Scer\GAL4ey.PH-mediated co-expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 ("eyeful" flies) results in excessively enlarged eyes, and eye tumours occur in 57% of eyes, with 3% of flies showing macroscopically visible metastases derived from the developing retina. Co-expression of atoScer\UAS.cJa almost completely suppresses the formation of eye tumours seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of atodsRNA.Scer\UAS.J results in a dramatic increase in tumour and metastases incidence in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC results in a dramatic increase in tumour and metastases incidence in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies: differentiated eye tissue is lost in 30% of the eye fields, and tumors are seen in 100% of the remaining eyes. These tumors include large patches of undifferentiated tissue. Co-expression of atoScer\UAS.cJa in 'eyeful' third instar eye discs (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) results in dramatically increased apoptosis, as measured by decay staining. Scer\GAL4ey.PH-mediated co-expression of DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 ("eyeful") third instar eye discs exhibit increased cell proliferation (as measured by phospho-histoneH3) posterior to the second mitotic wave. Co-expression of atoScer\UAS.cJa reduces ectopic proliferation in "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC increases ectopic proliferation in "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC in a Scer\GAL4ey.PH DlScer\UAS.cDa background leads to the appearance of significant numbers of proliferating cells in the posterior region of third instar eye discs. "Eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs exhibit disorganization of the epithelium. Co-expression of atoScer\UAS.cJa restores the size and organization of "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs. Co-expression of atoScer\UAS.T:Rep-en.T:Hsap\MYC severely worsens the retinal patterning of "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs, to the extent that the morphogenetic furrow is difficult to discriminate. Co-expression of dapScer\UAS.cUa leads to a significant inhibition of the tumor occurrence and a partial reduction in the metastasis seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies. Co-expression of bskDN.Scer\UAS in the 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background leads to tumors in 61% of eyes and an approximately 7-fold increase in metastasis. Co-expression of JraScer\UAS.cEa reduces tumor and metastasis incidence in the 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background. Co-expression of bskDN.Scer\UAS in a atoScer\UAS.cJa 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background leads to a suppression of the inhibitory effects of atoScer\UAS.cJa on the 'eyeful' tumor phenotype, restoring tumor formation and enhancing metastasis.
    Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) usually develop tumours in the eyes. Additionally, in approximately 30% of the flies, secondary eye growths are seen throughout the body, with the whole body filling up with eye tissue in some cases. The secondary eye growths have ragged borders, indicating invasion of the mutant tissue into the surrounding normal tissue. Eye discs show massive uncontrolled overgrowth in animals expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH). In most of the discs, the epithelial cells have lost their apical-basal polarity and some have a disrupted basement membrane and grow without differentiating. Wing discs of animals expressing DlScer\UAS.cDa under the control of Scer\GAL4dpp.blk1 and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4dpp.blk1) show massive overgrowth and mutant cells are found invading adjacent regions of the disc. Wing discs of animals expressing DlScer\UAS.cDa under the control of Scer\GAL4Bx-MS1096 and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4Bx-MS1096) show massive overgrowth, which initiates in the dorsal region but progressively extends to occupy the whole disc. Flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying psqGS88A8 and lolarev6 have reduced eye tumour size (compared to flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying psqGS88A8 and lolaGS88A8) but show sporadic secondary growth of eye tissue. The eye tumour phenotype of flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) is dominantly suppressed by one of Rpd304556, Su(var)3-917 or E(z)731, is dominantly enhanced by Rbf14, but is not enhanced by trxunspecified or ash1unspecified. The eye tumour phenotype of flies expressing DlScer\UAS.cDa under the control of Scer\GAL4ey.PH and also carrying the P{GSV1}lolaGS88A8 insertion (which results in the lolaGS88A8 and psqGS88A8 alleles being expressed under the control of Scer\GAL4ey.PH) is suppressed by co-expression of RbfScer\UAS.cDa.
    Xenogenetic Interactions
    Statement
    Reference
    Co-expression of Mmus\Atoh1Scer\UAS.cQa almost completely suppresses the formation of eye tumours seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies.
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    psqGS88A8
    Name Synonyms
    Secondary FlyBase IDs
      References (11)