Allele Dmel\Gr64e^MB03533

General Information
Symbol	Dmel\Gr64e^MB03533	Species	D. melanogaster
Name		FlyBase ID	FBal0192448
Feature type	allele	Associated gene	Dmel\Gr64e

Map ( GBrowse )	Untitled Document
Allele class
Mutagen
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:4,034,381..4,034,381 (Bellen, 2007.4.5, Wisotsky et al., 2011, Jiao et al., 2008)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	Mi{ET1}Gr64e^MB03533 (Bellen, 2007.4.5, Wisotsky et al., 2011, Jiao et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	feeding behavior defective (Wisotsky et al., 2011)
Phenotype Manifest In

Detailed Description
	Statement Reference Gr64e[MB03533] mutants show a marked loss of preference for 30% beer against 5mM (as in wild-type). Gr64e[MB03533] flies show a substantially reduced preference for yeast extract and fermented grape. The preference for ripe banana is indistinguishable from wild-type flies. Gr64e[MB03533] mutant flies show virtually no response to 10% glycerol, whereas control flies show a strong response. Gr64e[MB03533] flies have a responsive sugar neuron, as a stimulus of 100mM sucrose elicits a firing rate comparable to that in control flies. L-type sensilla in Gr64e[MB03533] flies lack a response to 10% glycerol. In contrast, mean responses to sucrose, fructose and glucose are as in wild-type. Gr64e[MB03533] flies show greatly reduced proboscis extension responses to glycerol. In feeding preference assays in which populations of flies are presented with 5mM sucrose against varying concentrations of glycerol, wild-type flies show strong preferences for 1% and 10% glycerol. (Wisotsky et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Gr64e^MB03533 is rescued by Gr64e^Scer\UAS.cWa/Scer\GAL4^Gr5a.8.5 (Wisotsky et al., 2011)
Comments	Expression of Gr64e[Scer\UAS.cWa] in sugar taste neurons (under the control of Scer\GAL4[Gr5a.8.5]) restores glycerol sensitivity, although only partial rescue is seen at a 1% glycerol concentration. (Wisotsky et al., 2011)
Stocks ( 1 )
Bloomington	23628 w¹¹¹⁸; Mi{ET1}Gr64e^MB03533 Gr64d^MB03533
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Gr64e^M (Jiao et al., 2008) Gr64e^MB03533 (Bellen, 2007.4.5)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Wisotsky et al., 2011, Nat. Neurosci. 14(12): 1534--1541 Evolutionary differences in food preference rely on Gr64e, a receptor for glycerol. [FBrf0216771] Jiao et al., 2008, Curr. Biol. 18(22): 1797--1801 Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila. [FBrf0206328]
Personal communication to FlyBase	Bellen, 2007.4.5, batch731.xml batch731.xml [FBrf0191801]

Allele Dmel\Gr64eMB03533

Allele Dmel\Gr64e^MB03533