FlyBase Allele Report: Scer\GAL4[Act5C.Switch.PR]

General Information

Symbol

Scer\GAL4^{Act5C.Switch.PR}

Species

S. cerevisiae

Name

FlyBase ID

FBal0192970

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Act5C(-FRT)GAL4.Switch.PR}

Also Known As

ActGS, act-GS-Gal4, Act5C-GS, Act5c-GS-Gal4, Act-GS-255B, GS-GAL4, GeneSwitch-GAL4, actin-GS-Gal4, actin-GeneSwitch-Gal4, act-gal4:PR

Transgenic product class

Mutagen

FLPase

Nature of the Allele

Transgenic product class

Mutagen

FLPase

(Rogulja and Irvine, 2005)

Progenitor genotype

Scer\GAL4^{FRT.Act5C.Switch.PR}

(Rogulja and Irvine, 2005)

Carried in construct

P{Act5C(-FRT)GAL4.Switch.PR}

Cytology

Description

The FRT cassette has been excised, allowing expression of the ligand-inducible GAL4::GS driver under the control of Act5C promoter sequences.

(Rogulja and Irvine, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

Act5C

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

organism | ubiquitous

(Huang et al., 2014)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression ubiquitously.

(FlyBase Curators, 2017)

ScerGAL4^{Act5C.Switch.PR} drives mifepristone-inducible expression ubiquitously.

(Huang et al., 2014)

Marker for

Reflects expression of

Act5C

(Huang et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal aging | RU486 conditional, with AhcyL1^HM05009

(Parkhitko et al., 2016)

abnormal courtship behavior | RU486 conditional, with wake^GD12087

(Chen et al., 2022)

abnormal courtship behavior | RU486 conditional, with wake^KK111313

(Chen et al., 2022)

abnormal courtship behavior | RU486 conditional, with wake^KK112014

(Chen et al., 2022)

abnormal immune response | RU486 conditional, with Atg5^RNAi.UAS

(Ren et al., 2009)

abnormal immune response | RU486 conditional, with Atg7^RNAi.UAS

(Ren et al., 2009)

abnormal immune response | RU486 conditional, with Atg12^RNAi.UAS

(Ren et al., 2009)

abnormal locomotor behavior | progressive | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

abnormal mating | RU486 conditional, with InR^K1409A.UAS

(Wigby et al., 2011)

abnormal oxidative stress response, with maf-S^RNAi.UAS

(Rahman et al., 2013)

abnormal oxidative stress response | female limited | RU486 conditional, with ATPsynD^GD9915

(Sun et al., 2014)

abnormal starvation stress response | adult stage | RU486 conditional, with Bcat^HMS01832

(Kim et al., 2023)

abnormal starvation stress response | adult stage | RU486 conditional, with Sec16^HMJ21252

(Zhang et al., 2022)

abnormal starvation stress response | female limited | RU486 conditional, with ATPsynD^GD9915

(Sun et al., 2014)

abnormal starvation stress response | RU486 conditional, with schlank^JF02502

(Nelson et al., 2016)

abnormal stress response, with COX5B^GD6659

(Zid et al., 2009)

abnormal stress response, with ND-SGDH^GD3876

(Zid et al., 2009)

abnormal stress response | female limited | RU486 conditional, with ImpL2^UAS.cHa

(Alic et al., 2011)

abnormal stress response | RU486 conditional, with et^GD6535

(Kallio et al., 2010)

abnormal stress response | RU486 conditional, with et^KK105015

(Kallio et al., 2010)

abnormal stress response | RU486 conditional, with Stat92E^GD4492

(Kallio et al., 2010)

chemical sensitive, with maf-S^RNAi.UAS

(Rahman et al., 2013)

decreased fecundity | female | RU486 conditional, with ImpL2^UAS.cHa

(Alic et al., 2011)

decreased occurrence of cell division | cell autonomous | somatic clone | third instar larval stage, with brk^UAS.cJa

(Rogulja and Irvine, 2005)

decreased occurrence of cell division | cell autonomous | somatic clone | third instar larval stage, with Dad^UAS.cTa

(Rogulja and Irvine, 2005)

female semi-sterile | progressive | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

increased cell death | somatic clone | RU486 conditional, with kibra^UAS.cBa

(Baumgartner et al., 2010)

increased cell death | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

increased cell number | cell non-autonomous | somatic clone | third instar larval stage, with tkv^{DE-Q199D.UAS.Tag:HA}

(Rogulja and Irvine, 2005)

increased cell number | cell non-autonomous | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

increased cell number | somatic clone | third instar larval stage, with tkv^{DE-Q199D.UAS.Tag:HA}

(Rogulja and Irvine, 2005)

increased cell number | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

increased fecundity | female | RU486 conditional, with AhcyL1^HM05009

(Parkhitko et al., 2016)

increased occurrence of cell division | cell autonomous | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

increased occurrence of cell division | cell non-autonomous | somatic clone | third instar larval stage, with brk^UAS.cJa

(Rogulja and Irvine, 2005)

increased occurrence of cell division | cell non-autonomous | somatic clone | third instar larval stage, with Dad^UAS.cTa

(Rogulja and Irvine, 2005)

increased occurrence of cell division | cell non-autonomous | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

increased occurrence of cell division | third instar larval stage, with tkv^{DE-Q199D.UAS.Tag:HA}

(Rogulja and Irvine, 2005)

increased rate of feeding behavior | adult stage | RU486 conditional, with Sec16^HMJ21252

(Zhang et al., 2022)

increased size | spermatogenesis | RU486 conditional, with Mettl3^GL01126

(Rockwell and Hongay, 2020)

lethal, with AdoR^UAS.cDa

(Kucerova et al., 2012)

lethal | RU486 conditional, with Surf1^RNAi.UAS

(Da-Rè et al., 2014)

lethal - all die before end of larval stage | RU486 conditional, with Ras85D^V12.UAS

(Shen et al., 2009)

lethal - all die before end of larval stage | RU486 conditional, with wg^{UAS.cHa.Tag:HA}

(Shen et al., 2009)

long lived, with Naglu^UAS.Tag:HA

(Xue et al., 2022)

long lived | :RU486 conditional | nutrition conditional, with htt^HMS02845

(Nelson et al., 2016)

long lived | female | RU486 conditional, with p53^259H.GUS

(Shen et al., 2009)

long lived | female limited | :RU486 conditional | nutrition conditional, with ATPsynD^GD9915

(Sun et al., 2014)

long lived | female limited | RU486 conditional, with ImpL2^UAS.cHa

(Alic et al., 2011)

long lived | female limited | RU486 conditional, with Pdk1^JF02807

(Danilov et al., 2015)

long lived | RU486 conditional, with AhcyL1^HM05009

(Parkhitko et al., 2016)

long lived | RU486 conditional, with AhcyL1^HMC04803

(Parkhitko et al., 2016)

long lived | RU486 conditional, with AhcyL2^HMJ23469

(Parkhitko et al., 2016)

long lived | RU486 conditional, with AhcyL2^NIG.8956R

(Parkhitko et al., 2016)

long lived | RU486 conditional, with fabp^UAS.cLa

(Lee et al., 2012)

long lived | RU486 conditional, with Hgd^HMC03775

(Parkhitko et al., 2020)

long lived | RU486 conditional, with Hpd^HMC03663

(Parkhitko et al., 2020)

long lived | RU486 conditional, with promL^GD2271

(Ryu et al., 2019)

long lived | RU486 conditional, with Rheb^HMS00923

(Parkhitko et al., 2016)

long lived | RU486 conditional, with Tat^HMC03212

(Parkhitko et al., 2020)

long lived | RU486 conditional, with Tat^HMS05877

(Parkhitko et al., 2020)

male semi-sterile | RU486 conditional, with Mettl3^GL01126

(Rockwell and Hongay, 2020)

short lived, with ImpL2^GX4499

(Paik et al., 2012)

short lived, with Naglu^HMC03368

(Xue et al., 2022)

short lived, with schlank^JF02502

(Nelson et al., 2016)

short lived | :nutrition conditional | RU486 conditional, with COX5B^GD6659

(Zid et al., 2009)

short lived | :nutrition conditional | RU486 conditional, with ND-SGDH^GD3876

(Zid et al., 2009)

short lived | :RU486 conditional | nutrition conditional, with htt^HMS02845

(Nelson et al., 2016)

short lived | :RU486 conditional | nutrition conditional, with hwt^GD15014

(Nelson et al., 2016)

short lived | male limited, with MRG15^GD11902

(Zhang et al., 2010)

short lived | RU486 conditional, with Ahcy^HMC03222

(Parkhitko et al., 2016)

short lived | RU486 conditional, with Atg1^GS10797

(Bjedov et al., 2020)

short lived | RU486 conditional, with BacA\p35^UAS.cHa

(Shen et al., 2009)

short lived | RU486 conditional, with Cbs^HMS03028

(Parkhitko et al., 2016)

short lived | RU486 conditional, with CG4743^HMC04321

(Parkhitko et al., 2016)

short lived | RU486 conditional, with CG8679^HMS01816

(Danilov et al., 2015)

short lived | RU486 conditional, with Hsap\LMNA^{progerin.UAS.A}

(Beard et al., 2008)

short lived | RU486 conditional, with Hsap\LMNA^UAS.A

(Beard et al., 2008)

short lived | RU486 conditional, with kuk^UASp.cBa

(Brandt et al., 2023)

short lived | RU486 conditional, with MetRS^HMC04303

(Parkhitko et al., 2016)

short lived | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

short lived | RU486 conditional, with PolrMT^UAS.GFP

(Liu et al., 2022)

short lived | RU486 conditional, with Ras85D^V12.UAS

(Shen et al., 2009)

short lived | RU486 conditional, with S6k^GL01327

(Danilov et al., 2015)

short lived | RU486 conditional, with S6k^HMS02267

(Danilov et al., 2015)

short lived | RU486 conditional, with wg^{UAS.cHa.Tag:HA}

(Shen et al., 2009)

some die during larval stage | RU486 conditional, with Ras85D^V12.UAS

(Shen et al., 2009)

some die during larval stage | RU486 conditional, with wg^{UAS.cHa.Tag:HA}

(Shen et al., 2009)

Phenotype Manifest In

adult brain, with Hsap\APP^{Aβ42.UAS.Tag:SS(nec).cBa}

(Xue et al., 2022)

egg | maternal effect | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

flight muscle cell | RU486 conditional, with kuk^UASp.GFP

(Brandt et al., 2023)

mitochondrion | maternal effect | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

mitochondrion | RU486 conditional, with PolrMT^{E423P.UAS.GFP}

(Liu et al., 2022)

nucleus | RU486 conditional, with kuk^UASp.GFP

(Brandt et al., 2023)

testis | RU486 conditional, with Mettl3^GL01126

(Rockwell and Hongay, 2020)

wing disc | cell non-autonomous | somatic clone | third instar larval stage, with tkv^{DE-Q199D.UAS.Tag:HA}

(Rogulja and Irvine, 2005)

wing disc | cell non-autonomous | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

wing disc | somatic clone | RU486 conditional, with kibra^UAS.cBa

(Baumgartner et al., 2010)

wing disc | somatic clone | third instar larval stage, with tkv^{DE-Q199D.UAS.Tag:HA}

(Rogulja and Irvine, 2005)

wing disc | somatic clone | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

wing disc | third instar larval stage, with tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Pi3K92E^A2860C.UAS, Scer\GAL4^{Act5C.Switch.PR}

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Scer\GAL4^{Act5C.Switch.PR}/chico^KK107581

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by InR^DN.UAS.cUa, Scer\GAL4^{Act5C.Switch.PR}

(Chen et al., 2022)

Enhancer of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Hsc70-4^UAS.cEa is an enhancer of bang sensitive phenotype of Tpi^sgk-1

(Hrizo and Palladino, 2010)

NOT Enhancer of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Atg12^RNAi.UAS is a non-enhancer of long lived phenotype of chico¹

(Bjedov et al., 2020)

Suppressor of

Statement

Reference

InR^DN.UAS.cUa, Scer\GAL4^{Act5C.Switch.PR} is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087

(Chen et al., 2022)

Pi3K92E^A2860C.UAS, Scer\GAL4^{Act5C.Switch.PR} is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}/chico^KK107581 is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}/InR^DN.UAS.cUa is a suppressor | partially of abnormal courtship behavior phenotype of Ecol\lexA^VP16.Ilp2.PL, wake^RNAi.lexAop

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}/Atg5^RNAi.UAS is a suppressor of long lived phenotype of chico¹

(Bjedov et al., 2020)

Scer\GAL4^{Act5C.Switch.PR}/Hsap\NGLY1^UAS.cGa is a suppressor of abnormal developmental rate | recessive phenotype of Pngl^PL

(Rodriguez et al., 2018)

Scer\GAL4^{Act5C.Switch.PR}/Hsap\NGLY1^UAS.cGa is a suppressor of decreased body size | recessive | adult stage phenotype of Pngl^PL

(Rodriguez et al., 2018)

Scer\GAL4^{Act5C.Switch.PR}/Hsc70-4^K71S.UAS is a suppressor of bang sensitive phenotype of Tpi^sgk-1

(Hrizo and Palladino, 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/Atg12^RNAi.UAS is a non-suppressor of long lived phenotype of chico¹

(Bjedov et al., 2020)

Scer\GAL4^{Act5C.Switch.PR}/BacA\p35^UAS.cHa is a non-suppressor of increased cell number | somatic clone | third instar larval stage phenotype of tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

Scer\GAL4^{Act5C.Switch.PR}/BacA\p35^UAS.cHa is a non-suppressor of increased cell number | somatic clone | cell non-autonomous | third instar larval stage phenotype of tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{Act5C.Switch.PR} has adult brain phenotype, suppressible | partially by Naglu^UAS.Tag:HA, Scer\GAL4^{Act5C.Switch.PR}

(Xue et al., 2022)

Suppressor of

Statement

Reference

Naglu^UAS.Tag:HA, Scer\GAL4^{Act5C.Switch.PR} is a suppressor | partially of adult brain phenotype of Hsap\APP^{Aβ42.UAS.Tag:SS(nec).cBa}, Scer\GAL4^{Act5C.Switch.PR}

(Xue et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}/BacA\p35^UAS.cHa is a non-suppressor of wing disc | somatic clone | third instar larval stage phenotype of tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

Scer\GAL4^{Act5C.Switch.PR}/BacA\p35^UAS.cHa is a non-suppressor of wing disc | somatic clone | cell non-autonomous | third instar larval stage phenotype of tkv^{Q253D.UAS.cNc}

(Rogulja and Irvine, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Act5C.Switch.PR}/step^UAS.FL rescues step^k08110

(Hahn et al., 2013)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9431

P{hsFLP}12, y¹ w^*; P{UAS-GFP.S65T}Myo31DF^T2; P{Act5C(-FRT)GAL4.Switch.PR}3/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

P{Act5C(-FRT)GAL4.Switch.PR}3 is reported as a stock (i.e.- germ-line transmitted), rather than just as somatic clones.

(Rogulja and Irvine, 2005)

Scer\GAL4^{Act5C.Switch.PR} was found to drive expression of responder transgenes even in the absence of RU486.

(Rodriguez et al., 2018)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ActGS

(Danilov et al., 2015)

Scer\GAL4^{Act5C.Switch.PR}

Name Synonyms

Secondary FlyBase IDs

References (58)

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