Allele Rnor\P2rx2^Scer\UAS.cLa

General Information
Symbol	Rnor\P2rx2^Scer\UAS.cLa	Species	R. norvegicus
Name	UAS construct of Lima	FlyBase ID	FBal0192973
Feature type	allele	Associated gene	Rnor\P2rx2

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Lima and Miesenbock, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS sequences control expression controls expression of Rnor\P2rx2. (Lima and Miesenbock, 2005)
Carried in construct	P{UAS-Rnor\P2rx2.L} (Lima and Miesenbock, 2005, Clyne and Miesenbock, 2008, Claridge-Chang et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective \| adult stage \| drug conditional, with Scer\GAL4^c17 (Lima and Miesenbock, 2005) locomotor behavior defective \| adult stage \| drug conditional, with Scer\GAL4^Cha.7.4 (Lima and Miesenbock, 2005) locomotor behavior defective \| adult stage \| drug conditional, with Scer\GAL4^ple.PF (Lima and Miesenbock, 2005) locomotor behavior defective \| adult stage \| drug conditional, with Scer\GAL4^{shakB.lethal.4.1} (Lima and Miesenbock, 2005) neurophysiology defective \| larval stage, with Scer\GAL4^Cha.7.4 (Lima and Miesenbock, 2005) neurophysiology defective \| larval stage \| drug conditional, with Scer\GAL4^Cha.7.4 (Lima and Miesenbock, 2005) paralytic \| adult stage \| drug conditional, with Scer\GAL4^Cha.7.4 (Lima and Miesenbock, 2005) short lived, with Scer\GAL4^Cha.7.4 (Lima and Miesenbock, 2005) uncoordinated, with Scer\GAL4^elav-C155 (Lima and Miesenbock, 2005) viable (Lima and Miesenbock, 2005) viable, with Scer\GAL4^nrv2.PS (Lima and Miesenbock, 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Action-contingent photoactivation of Rnor\P2rx2 in flies expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[ple.PF] produces conditioned odour avoidance. No conditioned odour avoidance is produced when the Scer\GAL4[Ddc.HL9] driver is used instead of Scer\GAL4[ple.PF]. (Claridge-Chang et al., 2009) Males in which all the fru-expressing neurons have been photoactivated (the flies express Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] and the Rnor\P2rx2 product is activated by release of DMNPE-caged ATP which is photolyzed by 100ms pulses of ultraviolet light) do show courtship behaviours, such as abdominal thrusting and unilateral wing vibrations, but only in a small fraction of 1.7% of all trials. However, if the ventral ganglion is isolated by physically severing the neck connectives, headless male torsos expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] sing readily and reliably when exposed to light; 46% of these animals show a song response. If the animals also carry Scer\GAL80[tsh-GAL80] (which represses Scer\GAL4[fru-GAL4] in the trunk but not in the descending neurons) then there is no song activity in response to photoactivation. The light-activated courtship songs of headless male torsos expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] are superficially indistinguishable from those of normal courting flies; one wing extends from the body at approximately a right angle and vibrates, and like in intact flies, the song is asymmetrical, with one wing or the other being relied on during repeated stimulation. The songs of these headless males have acoustic features of native courtship song, in particular, the presence of sine and pulse episodes. However, the pulse song of the headless males often lacks the metronomic precision and high-pulse repetition rates of courting flies. Recordings of these courtship songs substitute fully for the songs of courting males in an assay where the song is played back to fly pairs consisting of a wingless male (which cannot produce a courtship song) and a virgin female). Photoactivation of fru-expressing neurons in headless females expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] is able to elicit unilateral wing vibrations, but 4-fold higher photon does are needed on average to elicit a female "song" compared to headless males of the same genotype. Songs produced by these headless females are less cleanly structured than those of headless males, often lacking a stereotyped pulse waveform, sufficient dampening to limit pulses to three vibration cycles and stable sine frequencies below the approximately 220Hz flight oscillation. Spontaneous transitions to bilateral wing movements and high-intensity buzzing are common in the headless females. Recordings of these courtship songs do not substitute for the songs of courting males in an assay where the song is played back to fly pairs consisting of a wingless male (which cannot produce a courtship song) and a virgin female). (Clyne and Miesenbock, 2008) Development, behavior and viability of Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^nrv2.PS animals appears normal. Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^elav-C155 adults have subtle co-ordination defects. Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^Cha.7.4 adults die young (mean survival time after eclosion = 2.58 +/- 1.34 days, n = 329). Perfusion of ATP (μM 100) induces full scale excitatory junction potentials (EJPs) in abdominal muscles 6 or 7 of Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^Cha.7.4 wandering third instar larvae. These ATP induced EJPs are blocked by 200 nM tetrodotoxin, suggesting that they are action potential driven. In the absence of exogenous ATP these muscles show an increase in the frequency and amplitude of miniature EJPs compared to wild-type, but full scale EJPs are limited to occasional spontaneous bursts of activity. This response to ATP is not seen in the absence of Rnor\P2rx2^Scer\UAS.cLa. Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^{shakB.lethal.4.1} adults which have had 40-70 mM DMNPE-ATP() microinjected into their CNS exhibit typical giant fibre mediated escape movements (leg extension, jumping, wing opening, and high-frequency wing flapping) following 82% of brief exposures to UV laser light (8 mWmm^-2 for 150-250 ms). ( DMNPE-ATP is an ATP derivative from which free ATP can be release by exposure to 355 nm laser light.) This effect is not seen in Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^c370 or Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^c217 flies. Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^c17 adults which have had 40-70 mM DMNPE-ATP() microinjected into their CNS exhibit typical giant fibre mediated escape movements (leg extension, jumping, wing opening, and high-frequency wing flapping) following 63% of brief exposures to UV laser light (8 mWmm^-2 for 150-250 ms). ( DMNPE-ATP is an ATP derivative from which free ATP can be release by exposure to 355 nm laser light.) Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^Cha.7.4 adults which have had 40-70 mM DMNPE-ATP() microinjected into their CNS undergo convulsions and paralysis on exposure to UV laser light. ( DMNPE-ATP is an ATP derivative from which free ATP can be release by exposure to 355 nm laser light.) Rnor\P2rx2^Scer\UAS.cLa; Scer\GAL4^ple.PF flies whose nervous system has been micro-injected with 40-70 mM DMNPE-ATP() undergo marked and characteristic changes in behavior when exposed to UV laser-light. No such responses of locomotor activity to light stimulation is seen in controls. In the majority of such flies there is an instant increase in motor activity lasting approximately 30-120 seconds: the frequency and duration of pausing is reduced but average speed during periods of activity is unchanged. In addition, the types of routes the flies elect to follow changes after stimulation: frequently crisscrossing the center of the field rather than preferring to stay close to the perimeter as prior to stimulation. Occasionally, stimulated flies move in tightly wound circles. A minority of flies (32%) exhibit high motor activity prior to stimulation. For these flies, photo-stimulation leads to a transient arrest of locomotor activity. ( DMNPE-ATP is an ATP derivative from which free ATP can be release by exposure to 355 nm laser light.) (Lima and Miesenbock, 2005)
External Data
Linkouts
Interactions
Phenotypic Class
NOT suppressed by
Statement Reference Rnor\P2rx2^Scer\UAS.cLa, Scer\GAL4^c17/Scer\GAL4^c17 has locomotor behavior defective \| adult stage \| drug conditional phenotype, non-suppressible by norpA⁷/norpA⁷ (Lima and Miesenbock, 2005) Rnor\P2rx2^Scer\UAS.cLa, Scer\GAL4^{shakB.lethal.4.1} has locomotor behavior defective \| adult stage \| drug conditional phenotype, non-suppressible by norpA⁷/norpA⁷ (Lima and Miesenbock, 2005)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Photoactivation of fru-expressing neurons in headless females expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] in a fru[M]/fru[GAL4] background produces courtship songs with acoustic characteristics similar to those seen in headless males expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] in a fru[+] background. Recordings of these courtship songs substitute fully for the songs of courting males in an assay where the song is played back to fly pairs consisting of a wingless male (which cannot produce a courtship song) and a virgin female). Photoactivation of fru-expressing neurons in headless males expressing Rnor\P2rx2[Scer\UAS.cLa] under the control of Scer\GAL4[fru-GAL4] in a fru[F]/fru[GAL4] background produces courtship songs with sine and pulse songs, but the acoustic characteristics of these songs show broad bimodal distributions, with peaks near the typical male and female modes, suggesting that these animals represent a mixed population. (Clyne and Miesenbock, 2008) norpA⁷/norpA⁷ are blind and rarely fly. However, norpA⁷/norpA⁷ fails to suppress stimulation of the jump response, including flight behavior, by UV in Rnor\P2rx2^Scer\UAS.cLa adults carrying Scer\GAL4^c17 or Scer\GAL4^{shakB.lethal.4.1} which have had 40-70 mM DMNPE-ATP(*) microinjected into their CNS. (Lima and Miesenbock, 2005)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Rnor\P2rx2^Scer\UAS.cLa
Name Synonym	UAS construct of Lima
Secondary FlyBase IDs

References ( 3 )
Research paper	Claridge-Chang et al., 2009, Cell 139(2): 405--415 Writing memories with light-addressable reinforcement circuitry. [FBrf0208958] Clyne and Miesenbock, 2008, Cell 133(2): 354--363 Sex-specific control and tuning of the pattern generator for courtship song in Drosophila. [FBrf0204626] Lima and Miesenbock, 2005, Cell 121(1): 141--152 Remote control of behavior through genetically targeted photostimulation of neurons. [FBrf0187231]

Allele Rnor\P2rx2Scer\UAS.cLa

Allele Rnor\P2rx2^Scer\UAS.cLa