Allele Dmel\Prat2^{dsRNA.IR-AS.Scer\UAS}

General Information
Symbol	Dmel\Prat2^{dsRNA.IR-AS.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0193127
Feature type	allele	Associated gene	Dmel\Prat2

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Ji and Clark, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat of a 216bp Prat2 fragment (166bp of 5' UTR sequence and 50bp of the propeptide sequence) in an antisense-sense orientation, separated by a 95bp spacer. (Ji and Clark, 2006)
Carried in construct	P{UAS-Prat2.IR-AS} (Ji and Clark, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	female fertile, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006) lethal \| partially \| pupal stage, with Scer\GAL4^Act5C.PI (Ji and Clark, 2006) lethal \| pharate adult stage P15, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006)
Phenotype Manifest In
	abdomen \| P-stage, with Scer\GAL4^Act5C.PI (Ji and Clark, 2006) abdomen \| P-stage, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006) cephalopharyngeal skeleton \| P-stage, with Scer\GAL4^Act5C.PI (Ji and Clark, 2006) cephalopharyngeal skeleton \| P-stage, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006) head \| P-stage, with Scer\GAL4^Act5C.PI (Ji and Clark, 2006) head \| P-stage, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006) leg \| P-stage, with Scer\GAL4^αTub84B.PL (Ji and Clark, 2006)
Detailed Description
	Statement Reference Expression of Prat2^{dsRNA.IR-AS.Scer\UAS} under the control Scer\GAL4^Act5C.PI results in varying degrees of lethality, depending on the P{UAS-Prat.IR-AS} line used, the copy number and the temperature at which the flies are raised. Expression of Prat2^{dsRNA.IR-AS.Scer\UAS} under the control Scer\GAL4^αTub84B.PL results in 50% of arrested pupae having a "cryptocephal" phenotype, consisting of head eversion defects, legs that are not fully elongated, an abdomen that lacks cuticle and segmentation and larval mouthparts still being attached to the imaginal head structure. This cryptocephal phenotype is also seen in animals expressing Prat2^{dsRNA.IR-AS.Scer\UAS} under the control Scer\GAL4^Act5C.PI. Rarely, animals expressing Prat2^{dsRNA.IR-AS.Scer\UAS} under the control Scer\GAL4^Act5C.PI have a "microcephal" phenotype, which results from partial head eversion. These pupae retain their larval mouthparts and do not have a fully differentiated abdomen. Surviving female adults expressing Prat2^{dsRNA.IR-AS.Scer\UAS} under the control Scer\GAL4^αTub84B.PL are fertile. (Ji and Clark, 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has lethal \| pharate adult stage P15 phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has lethal \| prepupal stage P2 phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has lethal \| prepupal stage P4(i) phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has lethal \| pupal stage P5 phenotype (Ji and Clark, 2006)
Phenotype Manifest In
Other
Statement Reference Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has abdomen \| prepupal stage phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has abdomen \| P-stage phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has air bubble phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has embryonic/larval anterior spiracle phenotype (Ji and Clark, 2006) Prat2^{dsRNA.IR-AS.Scer\UAS}, Prat^{dsRNA.IR-AS.Scer\UAS}, Scer\GAL4^αTub84B.PL has head \| P-stage phenotype (Ji and Clark, 2006)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of both Prat2^{dsRNA.IR-AS.Scer\UAS} and Prat^{dsRNA.IR-AS.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in a higher degree of female lethality than that seen when either Prat2^{dsRNA.IR-AS.Scer\UAS} or Prat^{dsRNA.IR-AS.Scer\UAS} is expressed alone under the control of Scer\GAL4^αTub84B.PL. 27% of the double mutant animals arrest at a very early prepupal stage. Some of these arrested prepupae retain the thin and long shape typical of larvae, the anterior spiracle fails to evert and the gas bubble remains in the middle of the body. 34% of the double mutant animals arrest at prepupal stage P4(i) and have head eversion defects and a shrunken abdomen, and the gas bubble is in the posterior of the animal. 24% of the double mutant animals show head eversion, but the head is partially collapsed, resulting in a large cavity in the anterior of the pupa. 7% of the double mutant animals have a "cryptocephal" phenotype and 5% die as pharate adults. (Ji and Clark, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Prat2 gene. (Ji and Clark, 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Prat2^{dsRNA.IR-AS.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Ji and Clark, 2006, Genetics 172(3): 1621--1631 The purine synthesis gene Prat2 is required for Drosophila metamorphosis, as revealed by inverted-repeat-mediated RNA interference. [FBrf0190731]

Allele Dmel\Prat2dsRNA.IR-AS.Scer\UAS

Allele Dmel\Prat2^{dsRNA.IR-AS.Scer\UAS}