FB2025_01 , released February 20, 2025
Allele: Dmel\PratRNAi.AS.UAS
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General Information
Symbol
Dmel\PratRNAi.AS.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193128
Feature type
allele
Associated gene
Dmel\Prat
Associated Insertion(s)
Carried in Construct
P{UAS-Prat.RNAi.AS}
Key Links
Transgenic product class
RNAi_reagent
(Ji and Clark, 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ji and Clark, 2006)
Mutagen
in vitro construct
(Ji and Clark, 2006)
Progenitor genotype
Carried in construct
P{UAS-Prat.RNAi.AS}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of a 153bp Prat fragment (containing 5' UTR and propeptide sequence) in an antisense-sense orientation, separated by a 125bp spacer.

(Ji and Clark, 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Prat
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PratdsRNA.IR-AS.Scer\UAS under the control Scer\GAL4Act5C.PI results in 50-100% lethality, depending on the P{UAS-Prat.IR-AS} line used. Approximately half of surviving adult females expressing PratdsRNA.IR-AS.Scer\UAS under the control Scer\GAL4Act5C.PI are sterile.

      Most of the lethality caused by expression of PratdsRNA.IR-AS.Scer\UAS under the control Scer\GAL4αTub84B.PL occurs just before eclosion, although some animals arrest in early pupal stage P5. The arrested pharate adults have patches of necrotic tissue and/or missing abdominal tergites, but other external body structures are present.

      (Ji and Clark, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of both Prat2dsRNA.IR-AS.Scer\UAS and PratdsRNA.IR-AS.Scer\UAS under the control of Scer\GAL4αTub84B.PL results in a higher degree of female lethality than that seen when either Prat2dsRNA.IR-AS.Scer\UAS or PratdsRNA.IR-AS.Scer\UAS is expressed alone under the control of Scer\GAL4αTub84B.PL. 27% of the double mutant animals arrest at a very early prepupal stage. Some of these arrested prepupae retain the thin and long shape typical of larvae, the anterior spiracle fails to evert and the gas bubble remains in the middle of the body. 34% of the double mutant animals arrest at prepupal stage P4(i) and have head eversion defects and a shrunken abdomen, and the gas bubble is in the posterior of the animal. 24% of the double mutant animals show head eversion, but the head is partially collapsed, resulting in a large cavity in the anterior of the pupa. 7% of the double mutant animals have a "cryptocephal" phenotype and 5% die as pharate adults.

      (Ji and Clark, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Prat gene.

      (Ji and Clark, 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PratRNAi.AS.UAS
      PratRNAi.IR-AS.UAS
      PratdsRNA.IR-AS.Scer\UAS
      PratdsRNA.IR-AS.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)