Allele Dmel\bur^Scer\UAS.cLa

General Information
Symbol	Dmel\bur^Scer\UAS.cLa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Long	FlyBase ID	FBal0193130
Feature type	allele	Associated gene	Dmel\bur

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Long et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a full length bur cDNA (a 3.3kb fragment from EST RE18382). (Long et al., 2006)
Carried in construct	P{UAS-bur.L} (Long et al., 2006, van der Knaap et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Expression of bur[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] does not result in eye defects. (van der Knaap et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has visible phenotype, enhanceable by Bre1[+]/Bre1^kim1 (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has visible phenotype, enhanceable by Bre1^GD5127, Scer\GAL4^GMR.PF (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has visible phenotype, enhanceable by EcR^V559fs (van der Knaap et al., 2010)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has visible phenotype, suppressible \| partially by EcR^A483T (van der Knaap et al., 2010)
Other
Statement Reference Bre1[+]/Bre1^kim1, Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has partially lethal - majority die phenotype (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has visible phenotype (van der Knaap et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has eye phenotype, enhanceable by Bre1[+]/Bre1^kim1 (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has eye phenotype, enhanceable by Bre1^GD5127, Scer\GAL4^GMR.PF (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has eye phenotype, enhanceable by EcR^V559fs (van der Knaap et al., 2010)
Suppressed by
Statement Reference Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has eye phenotype, suppressible \| partially by EcR^A483T (van der Knaap et al., 2010)
Other
Statement Reference Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has eye phenotype (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has interommatidial bristle phenotype (van der Knaap et al., 2010) Scer\GAL4^GMR.PF, Usp7^Scer\UAS.cKa, bur^Scer\UAS.cLa has ommatidium phenotype (van der Knaap et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Simultaneous co-expression of bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF] results in defects in the eye characterised by disorganized or missing ommatidia, loss of bristles and black necrotic patches. Co-expression of Usp7[C250A.Scer\UAS] and bur[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] does not result in eye defects. Animals expressing bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF] and also heterozygous for Bre1[kim1] show strongly reduced viability. Surviving adults have a strongly enhanced mutant eye phenotype compared to animals expressing bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF] in an otherwise wild-type background. Animals expressing bur[Scer\UAS.cLa] under the control of Scer\GAL4[GMR.PF] in a Bre1[kim1]/+ background do not show eye defects. Co-expression of Bre1[GD5127] enhances the eye defects caused by co-expression of bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF]. EcR[V559fs] enhances the eye defects caused by co-expression of bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF]. EcR[A483T] partially suppresses the eye defects caused by co-expression of bur[Scer\UAS.cLa] and Usp7[Scer\UAS.cKa] under the control of Scer\GAL4[GMR.PF]. (van der Knaap et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	bur^Scer\UAS.cLa, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF partially rescues bur^e10 (Long et al., 2006) bur^Scer\UAS.cLa/Scer\GAL4^elav-C155 partially rescues bur^e10 (Long et al., 2006)
Comments	Expression of bur^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF or Scer\GAL4^elav-C155 almost completely rescues the R-cell guidance defects seen in bur^e10 mutants. Expression of bur^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 substantially rescues the pupal lethality of bur^e10 homozygotes. (Long et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	bur^Scer\UAS.cLa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Long
Secondary FlyBase IDs

References ( 2 )
Research paper	van der Knaap et al., 2010, Mol. Cell. Biol. 30(3): 736--744 Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. [FBrf0209736] Long et al., 2006, Genetics 172(3): 1633--1642 De novo GMP synthesis is required for axon guidance in Drosophila. [FBrf0190740]

Allele Dmel\burScer\UAS.cLa

Allele Dmel\bur^Scer\UAS.cLa