Allele Dmel\Pink1^B9

General Information
Symbol	Dmel\Pink1^B9	Species	D. melanogaster
Name		FlyBase ID	FBal0193144
Feature type	allele	Associated gene	Dmel\Pink1
Also Known As	dPink1^B9

Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Park et al., 2006)
Mutagen	P-element activity (Park et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Pink1^GE271 (Park et al., 2006)
Nature of the lesion	Statement Reference Imprecise excision of the insertion, resulting in a deletion of 570bp of Pink1 sequences. (Park et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	flight defective (Vilain et al., 2012, Vos et al., 2012) flightless (Park et al., 2006, Park et al., 2009) heat stress response defective (Ishida et al., 2012) locomotor behavior defective (Park et al., 2006, Tain et al., 2009, Imai et al., 2010) locomotor behavior defective \| adult stage (Costa et al., 2013) male sterile (Vilain et al., 2012) short lived (Imai et al., 2010) visible (Park et al., 2006, Park et al., 2009, Yang et al., 2008, Imai et al., 2010, Costa et al., 2013)
Phenotype Manifest In
	adult thorax (Park et al., 2006, Park et al., 2009, Imai et al., 2010, Costa et al., 2013) dopamine neuron & adult brain (Park et al., 2006) dopaminergic PPL1 neuron (Imai et al., 2010) dopaminergic PPM1 neuron (Imai et al., 2010) dopaminergic PPM2 neuron (Imai et al., 2010) indirect flight muscle (Vilain et al., 2012, Park et al., 2006, Yang et al., 2008) mitochondrial inner membrane & indirect flight muscle (Yang et al., 2008) mitochondrial outer membrane & indirect flight muscle (Park et al., 2006) mitochondrion (Vilain et al., 2012, Park et al., 2006, Imai et al., 2010, Vos et al., 2012, Costa et al., 2013) mitochondrion & dopamine neuron & adult brain (Park et al., 2006) mitochondrion & dopaminergic neuron (Park et al., 2009) mitochondrion & dorso-lateral dopaminergic neuron (Yang et al., 2008) mitochondrion & indirect flight muscle (Park et al., 2006, Yang et al., 2008) myofibril & indirect flight muscle (Park et al., 2006) Nebenkern (Park et al., 2006) spermatozoon (Park et al., 2006) wing (Park et al., 2006, Park et al., 2009, Yang et al., 2008, Imai et al., 2010)
Detailed Description
	Statement Reference 10 day old mutant flies have impaired climbing ability compared to controls. Mutant flies have thoracic indentations. (Costa et al., 2013) Pink1[B9] flies are sensitive to heat shock stress and die sooner than control flies. (Ishida et al., 2012) The mitochondria in the indirect flight muscles of mutant adults appear enlarged and clumped compared to controls. (Vilain et al., 2012) The flight defects of mutant animals are significantly improved by feeding with vitamin K[[2]] (MK-4 and MK-8 forms tested). The mitochondrial morphology defects of mutant larvae are partially rescued by feeding with vitamin K[[2]] (MK-4 form tested). (Vos et al., 2012) Mutant adults often have abnormal thoraces with dents in the mid-anterior region. As the mutant adults age, they develop abnormal wing postures, with the percentage of affected flies increasing with age. The mutant adults also show a progressive loss of climbing ability and have a shorter lifespan than normal. Mitochondria in the indirect flight muscles of one day old mutant adults are abnormally fused with one another and the structures of the mitochondrial cristae are unclear. Mitochondria in the mutant dopaminergic neurons of the adult brain form spherical aggregates (in contrast to the long tubular network seen in wild type). 25 day old adults show loss of dopaminergic neurons in the protocerebral posterior lateral 1 (PPL1) and protocerebral posterior medial 1 and 2 (PPM1/2) clusters of the brain. (Imai et al., 2010) Mutant flies have a downturned wing position and crushed thorax and are flightless. Mutants have unusually large swollen mitochondria in the dopaminergic neurons. (Park et al., 2009) 5 day old adult mutant flies have an abnormal wing posture. Indirect flight muscles are thin, atrophic and disorganised in these animals. Some of the mitochondria in the indirect flight muscles appear grossly enlarged, with the inner membrane being disorganised or disintegrated. Prominent mitochondrial aggregates form in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in Pink1[B9] animals, and in addition tubular mitochondrial structure are also seen. (Yang et al., 2008) Mutant males have impaired sperm with swollen Nebenkern. 3 day old flies often show a downturned wing phenotype and they completely lack flight ability. Mutant adults show reduced climbing ability compared to controls both at 3 days and 30 days after eclosion. 85% of adults show a collapsed-thorax phenotype immediately after eclosion, particularly in the mid-anterior and anterolateral regions of the thorax. The percentage of flies showing this phenotype increases to 95% at 30 days after eclosion. The indirect flight muscles are reduced compared to wild-type and have an abnormal structure; the myofibrils are arranged irregularly and the mitochondria are immensely swollen with loss of the outer membrane. Apoptotic cells are detected in mutant indirect flight muscles but not in wild-type controls. The number of dopaminergic neurons in the dorsomedial and dorsolateral 1 (DL1) clusters of the brain is slightly but significantly reduced compared to controls in 30 day old adult flies. The number of dopaminergic neurons in the DL2 and posteriomedial clusters is not significantly altered in these animals. 3 day old Pink1^B9 adults have a number of profoundly enlarged mitochondria in dopaminergic neurons in the dorsomedial, dorsolateral 1 and 2 and posteriomedial clusters of the brain. This enlarged mitochondrion phenotype progressively increases with age. (Park et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Pink1^B9 has flight defective phenotype, enhanceable by heix[+]/heix¹ (Vos et al., 2012) Pink1^B9 has flight defective phenotype, enhanceable by heix[+]/heix^NP5301 (Vos et al., 2012) Pink1^B9 has flight defective phenotype, enhanceable by heix²/heix[+] (Vos et al., 2012) Pink1^B9 has flight defective phenotype, enhanceable by heix^k11403/heix[+] (Vos et al., 2012) Pink1^B9 has heat stress response defective phenotype, enhanceable by Pgam5¹ (Ishida et al., 2012) Pink1^B9 has locomotor behavior defective phenotype, enhanceable by Pgam5^Scer\UAS.cIa/Scer\GAL4^da.G32 (Imai et al., 2010) Pink1^B9 has short lived phenotype, enhanceable by Pgam5^Scer\UAS.cIa/Scer\GAL4^da.G32 (Imai et al., 2010) Pink1^B9 has visible phenotype, enhanceable by Pgam5^Scer\UAS.cIa/Scer\GAL4^da.G32 (Imai et al., 2010) Pink1^B9 has visible phenotype, enhanceable by Scer\GAL4^hs.PB/Drp1^{K38A.Scer\UAS.T:Ivir\HA1} (Park et al., 2009)
NOT Enhanced by
Statement Reference Pink1^B9 has locomotor behavior defective phenotype, non-enhanceable by HtrA2^Δ1 (Tain et al., 2009) Pink1^B9 has locomotor behavior defective phenotype, non-enhanceable by park²⁵ (Tain et al., 2009)
Suppressed by
Statement Reference Pink1^B9 has flight defective phenotype, suppressible \| partially by Drp1^+t9.4 (Vilain et al., 2012) Pink1^B9 has flight defective phenotype, suppressible \| partially by opa1-like^s3475 (Vilain et al., 2012) Pink1^B9 has flight defective phenotype, suppressible \| partially by Scer\GAL4^da.G32/heix^Scer\UAS.cVa (Vos et al., 2012) Pink1^B9 has flight defective phenotype, suppressible \| partially by Scer\NDI1^Scer\UAS.cVa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has flightless phenotype, suppressible \| partially by Marf^GD11094/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has flightless phenotype, suppressible \| partially by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has flightless phenotype, suppressible by Drp1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has locomotor behavior defective \| adult stage phenotype, suppressible by Scer\GAL4^da.G32/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has locomotor behavior defective \| adult stage phenotype, suppressible by Scer\GAL4^elav.PU/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has locomotor behavior defective phenotype, suppressible by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has locomotor behavior defective phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has locomotor behavior defective phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has locomotor behavior defective phenotype, suppressible by Scer\GAL4^Act.PU/HtrA2^{Scer\UAS.P\T.cTa} (Tain et al., 2009) Pink1^B9 has male sterile phenotype, suppressible by Scer\NDI1^Scer\UAS.cVa (Vilain et al., 2012) Pink1^B9 has short lived phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has short lived phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has short lived phenotype, suppressible by Scer\GAL4^da.G32/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has visible phenotype, suppressible \| partially by Scer\GAL4^da.G32/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has visible phenotype, suppressible \| partially by Scer\GAL4^elav.PU/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has visible phenotype, suppressible by Drp1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has visible phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has visible phenotype, suppressible by Marf^GD11094/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has visible phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Park et al., 2009, Yang et al., 2008) Pink1^B9 has visible phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has visible phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010)
NOT suppressed by
Statement Reference Pink1^B9 has flight defective phenotype, non-suppressible by Buffy^Scer\UAS.cQa/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has flight defective phenotype, non-suppressible by Zzzz\AOX^Scer\UAS.cFa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has flightless phenotype, non-suppressible by Drp1²/Drp1[+] (Park et al., 2009) Pink1^B9 has flightless phenotype, non-suppressible by opa1-like[+]/opa1-like^EY09863 (Park et al., 2009) Pink1^B9 has flightless phenotype, non-suppressible by opa1-like^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has flightless phenotype, non-suppressible by Scer\GAL4^hs.PB/Drp1^{K38A.Scer\UAS.T:Ivir\HA1} (Park et al., 2009) Pink1^B9 has locomotor behavior defective phenotype, non-suppressible by HtrA2^Δ1 (Tain et al., 2009) Pink1^B9 has locomotor behavior defective phenotype, non-suppressible by park²⁵ (Tain et al., 2009) Pink1^B9 has male sterile phenotype, non-suppressible by Drp1^+t9.4 (Vilain et al., 2012) Pink1^B9 has male sterile phenotype, non-suppressible by opa1-like^s3475 (Vilain et al., 2012) Pink1^B9 has male sterile phenotype, non-suppressible by Zzzz\AOX^Scer\UAS.cFa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has visible phenotype, non-suppressible by Drp1²/Drp1[+] (Park et al., 2009) Pink1^B9 has visible phenotype, non-suppressible by opa1-like^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^hs.PB (Park et al., 2009)
Enhancer of
Statement Reference Pink1^B9 is an enhancer of heat stress response defective phenotype of Pgam5¹ (Ishida et al., 2012)
NOT Enhancer of
Statement Reference Pink1^B9 is a non-enhancer of locomotor behavior defective phenotype of park²⁵ (Tain et al., 2009)
Suppressor of
Statement Reference Pink1^B9 is a suppressor \| partially of visible phenotype of Scer\GAL4^GMR.PF, rho-7^Scer\UAS.cWa (Whitworth et al., 2008)
NOT Suppressor of
Statement Reference Pink1^B9 is a non-suppressor of locomotor behavior defective phenotype of park²⁵ (Tain et al., 2009) Pink1^B9 is a non-suppressor of visible phenotype of HtrA2^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Whitworth et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference Pink1^B9 has adult thorax phenotype, enhanceable by Scer\GAL4^hs.PB/Drp1^{K38A.Scer\UAS.T:Ivir\HA1} (Park et al., 2009) Pink1^B9 has wing phenotype, enhanceable by Pgam5^Scer\UAS.cIa/Scer\GAL4^da.G32 (Imai et al., 2010) Pink1^B9 has wing phenotype, enhanceable by Scer\GAL4^hs.PB/Drp1^{K38A.Scer\UAS.T:Ivir\HA1} (Park et al., 2009)
Suppressed by
Statement Reference Pink1^B9, park¹ has mitochondrion phenotype, suppressible \| partially by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Kim et al., 2008) Pink1^B9, park¹ has mitochondrion phenotype, suppressible \| partially by park^{T187A.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Kim et al., 2008) Pink1^B9, park¹ has mitochondrion phenotype, suppressible by park^{Scer\UAS.T:Zzzz\FLAG,T:Hsap\COX4I1}/Scer\GAL4^hs.PB (Kim et al., 2008) Pink1^B9 has adult thorax phenotype, suppressible \| partially by Buffy^Scer\UAS.cQa/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has adult thorax phenotype, suppressible \| partially by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has adult thorax phenotype, suppressible \| partially by Scer\GAL4^da.G32/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has adult thorax phenotype, suppressible \| partially by Scer\GAL4^elav.PU/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has adult thorax phenotype, suppressible by Drp1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has adult thorax phenotype, suppressible by Marf^GD11094/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has adult thorax phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Park et al., 2009) Pink1^B9 has adult thorax phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has adult thorax phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has dopamine neuron & adult brain phenotype, suppressible \| partially by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has dopaminergic PPL1 neuron phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has dopaminergic PPL1 neuron phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has dopaminergic PPM1 neuron phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has dopaminergic PPM1 neuron phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has dopaminergic PPM2 neuron phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has dopaminergic PPM2 neuron phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has indirect flight muscle phenotype, suppressible \| partially by Buffy^Scer\UAS.cQa/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has indirect flight muscle phenotype, suppressible \| partially by Scer\NDI1^Scer\UAS.cVa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has indirect flight muscle phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has indirect flight muscle phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Yang et al., 2008) Pink1^B9 has indirect flight muscle phenotype, suppressible by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has mitochondrial inner membrane & indirect flight muscle phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has mitochondrial inner membrane & indirect flight muscle phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Yang et al., 2008) Pink1^B9 has mitochondrion & dopamine neuron & adult brain phenotype, suppressible \| partially by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ple.PF (Park et al., 2006) Pink1^B9 has mitochondrion & dopaminergic neuron phenotype, suppressible by Drp1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ple.PF (Park et al., 2009) Pink1^B9 has mitochondrion & dopaminergic neuron phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Park et al., 2009) Pink1^B9 has mitochondrion & dopaminergic neuron phenotype, suppressible by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ple.PF (Park et al., 2009) Pink1^B9 has mitochondrion & dorso-lateral dopaminergic neuron phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has mitochondrion & dorso-lateral dopaminergic neuron phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Yang et al., 2008) Pink1^B9 has mitochondrion & dorso-lateral dopaminergic neuron phenotype, suppressible by park^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ple.PF (Yang et al., 2008) Pink1^B9 has mitochondrion & indirect flight muscle phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has mitochondrion & indirect flight muscle phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Yang et al., 2008) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Buffy^Scer\UAS.cQa/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Pgam5¹ (Imai et al., 2010) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Pgam5^NP0568 (Imai et al., 2010) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Scer\GAL4^da.G32/heix^Scer\UAS.cVa (Vos et al., 2012) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Scer\GAL4^da.G32/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Scer\GAL4^elav.PU/Trap1^Scer\UAS.cCa (Costa et al., 2013) Pink1^B9 has mitochondrion phenotype, suppressible \| partially by Scer\NDI1^Scer\UAS.cVa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has wing phenotype, suppressible \| partially by Buffy^Scer\UAS.cQa/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has wing phenotype, suppressible by Drp1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has wing phenotype, suppressible by Drp1^{T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1} (Yang et al., 2008) Pink1^B9 has wing phenotype, suppressible by Marf^GD11094/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has wing phenotype, suppressible by opa1-like[+]/opa1-like^EY09863 (Park et al., 2009, Yang et al., 2008) Pink1^B9 has wing phenotype, suppressible by park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB (Park et al., 2006) Pink1^B9 has wing phenotype, suppressible by Pgam5¹ (Imai et al., 2010) Pink1^B9 has wing phenotype, suppressible by Pgam5^NP0568 (Imai et al., 2010)
NOT suppressed by
Statement Reference Pink1^B9 has adult thorax phenotype, non-suppressible by Drp1²/Drp1[+] (Park et al., 2009) Pink1^B9 has adult thorax phenotype, non-suppressible by opa1-like^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^hs.PB (Park et al., 2009) Pink1^B9 has indirect flight muscle phenotype, non-suppressible by Zzzz\AOX^Scer\UAS.cFa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has mitochondrion & dopaminergic neuron phenotype, non-suppressible by Drp1²/Drp1[+] (Park et al., 2009) Pink1^B9 has mitochondrion phenotype, non-suppressible by Zzzz\AOX^Scer\UAS.cFa/Scer\GAL4^da.G32 (Vilain et al., 2012) Pink1^B9 has wing phenotype, non-suppressible by Drp1²/Drp1[+] (Park et al., 2009) Pink1^B9 has wing phenotype, non-suppressible by opa1-like^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^hs.PB (Park et al., 2009)
Suppressor of
Statement Reference Pink1^B9 is a suppressor \| partially of eye phenotype of Scer\GAL4^GMR.PF, rho-7^Scer\UAS.cWa (Whitworth et al., 2008) Pink1^B9 is a suppressor \| partially of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, rho-7^Scer\UAS.cWa (Whitworth et al., 2008)
NOT Suppressor of
Statement Reference Pink1^B9 is a non-suppressor of eye phenotype of HtrA2^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Whitworth et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference Expression of Trap1[Scer\UAS.cCa] under the control of either Scer\GAL4[da.G32] or Scer\GAL4[elav.PU] partially suppresses the thoracic indentation phenotype which is seen in Pink1[B9] flies. Expression of Trap1[Scer\UAS.cCa] under the control of Scer\GAL4[da.G32] in Pink1[B9] flies improves their climbing ability, lifespan, resistance to paraquat and mitochondrial function. Expression of Trap1[Scer\UAS.cCa] under the control of Scer\GAL4[elav.PU] improves climbing ability and mitochondrial respiration in Pink1[B9] flies. (Costa et al., 2013) Pgam5[1] Pink1[B9] double mutant flies are more sensitive to heat shock stress than either single mutant alone. (Ishida et al., 2012) The mitochondrial morphology defects of Pink1[B9] larvae are partially rescued by expression of heix[Scer\UAS.cVa] under the control of Scer\GAL4[da.G32]. (Vos et al., 2012) The defects in the adult thorax, the progressive abnormal wing posture phenotype, the progressive loss of climbing ability and the shorter lifespan seen in Pink1[B9] flies are suppressed if they are also mutant for either Pgam5[NP0568] or Pgam5[1]. The progressive abnormal wing posture phenotype, the progressive loss of climbing ability and the reduction in lifespan seen in Pink1[B9] flies are enhanced by expression of Pgam5[Scer\UAS.cIa] under the control of Scer\GAL4[da.G32]. The mitochondrial hyperfusion and loss of cristae of the mitochondria of indirect flight muscles of one day old Pink1[B9] adults is partially suppressed by either Pgam5[NP0568] or Pgam5[1]. The loss of dopaminergic neurons in the protocerebral posterior lateral 1 (PPL1) and protocerebral posterior medial 1 and 2 (PPM1/2) clusters of the brain that is seen in 25 day old Pink1[B9] adults is suppressed if the flies are also mutant for Pgam5[NP0568] or Pgam5[1]. (Imai et al., 2010) The downturned wing, crushed thorax and flightless phenotypes of Pink1[B9] mutant flies are suppressed by expression of Drp1[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[hs.PB]. The downturned wing and crushed thorax phenotypes of Pink1[B9] mutant flies are enhanced by expression of Drp1[K38A.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[hs.PB]. The downturned wing, crushed thorax and flightless phenotypes of Pink1[B9] mutant flies are not suppressed by Drp1[2]/+. The downturned wing, crushed thorax and flightless phenotypes of Pink1[B9] mutant flies are not suppressed by expression of opa1-like[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[hs.PB]. The downturned wing and crushed thorax phenotypes but not the flightless phenotype of Pink1[B9] mutant flies is significantly suppressed by opa1-like[EY09863]/+. Expression of Marf[GD11094] under the control of Scer\GAL4[hs.PB] strongly suppresses the downturned wing and crushed thorax phenotypes of Pink1[B9] mutant flies and the flightless phenotype is partially suppressed. The downturned wing, crushed thorax and flightless phenotypes of Pink1[B9] mutant flies are not suppressed by expression of Marf[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[hs.PB]. The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1[B9] mutants is suppressed by expression of one of park[Scer\UAS.T:Hsap\MYC] or Drp1[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[ple.PF]. The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1[B9] mutants is not suppressed by Drp1[2]/+ but is significantly rescued by opa1-like[EY09863]/+. (Park et al., 2009) The climbing ability of park[25] Pink1[B9] double mutants is not significantly different from the climbing ability of either single mutant. The climbing ability of HtrA2[Δ1] Pink1[B9] double mutants is not significantly different from the climbing ability of the Pink1[B9] single mutant. The defects in climbing ability that are seen in Pink1[B9] mutants are suppressed by expression of HtrA2[Scer\UAS.P\T.cTa] under the control of Scer\GAL4[Act]. (Tain et al., 2009) Expression of park[Scer\UAS.T:Zzzz\FLAG,T:Hsap\COX4I1] under the control of Scer\GAL4[hs.PB] suppresses the mitochondrial swelling phenotype in Pink1[B9], park[1] double mutants to a great extent. Expression of park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] partially suppresses the mitochondrial swelling phenotype in Pink1[B9], park[1] double mutants. Expression of park[T187A.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] partially suppresses the mitochondrial swelling phenotype in Pink1[B9], park[1] double mutants. (Kim et al., 2008) Pink1[B9] does not suppress the eye pigmentation loss seen in flies expressing HtrA2[Scer\UAS.cPa] under the control of Scer\GAL4[GMR.PF]. Pink1[B9] partially suppresses the rough eye phenotype seen when rho-7[Scer\UAS.cWa] is expressed under the control of Scer\GAL4[GMR.PF]. The normal abundance of interommatidial bristles is restored. (Whitworth et al., 2008) The abnormal wing posture phenotype and the abnormal morphology of the indirect flight muscles and their mitochondria which is seen in Pink1[B9] flies is suppressed by Drp1[T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1] or by opa1-like[EY09863]/+. The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in Pink1[B9] animals is suppressed by Drp1[T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1] or by opa1-like[EY09863]/+. The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in Pink1[B9] animals is suppressed by expression of park[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[ple.PF]. (Yang et al., 2008) Expression of Buffy^Scer\UAS.cQa under the control of Scer\GAL4^hs.PB markedly suppresses the adult wing and thorax phenotypes seen in Pink1^B9 flies. The indirect flight muscle defects are also suppressed in these animals, although the flight defect is not rescued. Expression of park^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB markedly suppresses the downturned wing and crushed thorax phenotypes seen in Pink1^B9 flies. The indirect flight muscle defects and the loss of dopaminergic neurons in the adult brain are also suppressed in these animals. The rescued flies show increased climbing speed and have some flight ability. Expression of park^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ple.PF significantly suppresses the enlarged mitochondria phenotype seen in the dopaminergic neurons of Pink1^B9 adult brains. The thoracic muscle defects seen in park¹ Pink1^B9 double mutant flies are not increased in severity compared to either single mutant. (Park et al., 2006)
Xenogenetic Interactions
Statement Reference Scer\NDI1[Scer\UAS.cVa] (in the absence of a Scer\GAL4 driver) rescues the sterility of Pink1[B9] male flies. Expression of Scer\NDI1[Scer\UAS.cVa] under the control of Scer\GAL4[da.G32] partially suppresses the flight defects of Pink1[B9] flies. The defects in organisation of the mitochondria which are seen in the indirect flight muscles of Pink1[B9] flies are also partially suppressed by expression of Scer\NDI1[Scer\UAS.cVa] under the control of Scer\GAL4[da.G32]. Expression of Zzzz\AOX[Scer\UAS.cFa] under the control of Scer\GAL4[da.G32] does not suppress the male sterility, flight defects or defects in mitochondrial morphology seen in Pink1[B9] flies. (Vilain et al., 2012)
Complementation & Rescue Data
Rescued by	Pink1^B9 is rescued by Pink1^{Scer\UAS.T:Ivir\HA1} (Kim et al., 2008)
Partially rescued by	Pink1^B9 is partially rescued by Pink1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^hs.PB (Park et al., 2006)
Not rescued by	Pink1^B9 is not rescued by Pink1^{K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1} (Kim et al., 2008)
Comments	Expression of Pink1[K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[hs.PB] fails to rescue the apparent behavioural phenotypes of Pink1[B9] mutants. Impaired mitochondria in the indirect flight muscle of Pink1[B9] mutants are not recovered by Pink1[K337R.D479A.D501A.Scer\UAS.T:Ivir\HA1] expression under the control of Scer\GAL4[hs.PB]. Expression of Pink1[Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[hs.PB] almost fully rescues the apparent behavioural phenotypes of Pink1[B9] mutants. Similarly, impaired mitochondria in the indirect flight muscle of Pink1[B9] mutants are fully recovered by Pink1[Scer\UAS.T:Ivir\HA1] expression under the control of Scer\GAL4[hs.PB]. (Kim et al., 2008)
Stocks ( 1 )
Bloomington	34749 w^* Pink1^B9/FM7i, P{ActGFP}JMR3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	dPink1^B9 (Yang et al., 2008, Yang et al., 2008) Pink1^B9 (Costa et al., 2013, Kim et al., 2012) PINK1^B9 (Park et al., 2006, Kim et al., 2008, Ishida et al., 2012, Park et al., 2009, Tain et al., 2009, Lee et al., 2007, Imai et al., 2010, Ziviani et al., 2010) pink1^B9 (Vilain et al., 2012, Whitworth et al., 2008, Vos et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 15 )
Research paper	Costa et al., 2013, Cell Death Dis. 4: e467 Drosophila Trap1 protects against mitochondrial dysfunction in a PINK1/parkin model of Parkinson's disease. [FBrf0220561] Ishida et al., 2012, PLoS ONE 7(2): e30265 Prevention of Apoptosis by Mitochondrial Phosphatase PGAM5 in the Mushroom Body Is Crucial for Heat Shock Resistance in Drosophila melanogaster. [FBrf0217492] Kim et al., 2012, J. Biol. Chem. 287(9): 6628--6641 Glutathione s-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. [FBrf0217543] Vilain et al., 2012, PLoS Genet. 8(1): e1002456 The Yeast Complex I Equivalent NADH Dehydrogenase Rescues pink1 Mutants. [FBrf0217195] Vos et al., 2012, Science 336(6086): 1306--1310 Vitamin K2 is a mitochondrial electron carrier that rescues pink1 deficiency. [FBrf0218543] Imai et al., 2010, PLoS Genet. 6(12): e1001229 The Loss of PGAM5 Suppresses the Mitochondrial Degeneration Caused by Inactivation of PINK1 in Drosophila. [FBrf0212500] Ziviani et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(11): 5018--5023 Drosophila Parkin requires PINK1 for mitochondrial translocation and ubiquitinates Mitofusin. [FBrf0210308] Park et al., 2009, Biochem. Biophys. Res. Commun. 378(3): 518--523 The PINK1-Parkin pathway is involved in the regulation of mitochondrial remodeling process. [FBrf0207317] Tain et al., 2009, Cell Death Differ. 16(8): 1118--1125 Drosophila HtrA2 is dispensable for apoptosis but acts downstream of PINK1 independently from Parkin. [FBrf0208357] Kim et al., 2008, Biochem. Biophys. Res. Commun. 377(3): 975--980 PINK1 controls mitochondrial localization of Parkin through direct phosphorylation. [FBrf0207333] Whitworth et al., 2008, Dis. Model Mech. 1(2-3): 168--174 Rhomboid-7 and HtrA2/Omi act in a common pathway with the Parkinson's disease factors Pink1 and Parkin. [FBrf0206306] Yang et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(19): 7070--7075 Pink1 regulates mitochondrial dynamics through interaction with the fission/fusion machinery. [FBrf0205047] Lee et al., 2007, Biochem. Biophys. Res. Commun. 358(2): 534--539 Loss of LRRK2/PARK8 induces degeneration of dopaminergic neurons in Drosophila. [FBrf0201118] Park et al., 2006, Nature 441(7097): 1157--1161 Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin. [FBrf0193630]
Erratum	Yang et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(45): 17585 Correction for Yang et al., Pink1 regulates mitochondrial dynamics through interaction with the fission/fusion machinery. [FBrf0208866]

Allele Dmel\Pink1B9

Allele Dmel\Pink1^B9