Allele Dmel\Sp7^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Sp7^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0193154
Feature type	allele	Associated gene	Dmel\Sp7

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Castillejo-Lopez and Haecker, 2005) in vitro construct - RNAi (Castillejo-Lopez and Haecker, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 729bp genomic fragment of Sp7 (which includes the last 3 introns) and a 425bp cDNA sequence of Sp7 arranged in opposite orientation. The construct is expected to produce a dsRNA composed of a 425bp hairpin linked by a 148 nucleotide loop. (Castillejo-Lopez and Haecker, 2005)
Carried in construct	P{UAS-Sp7.dsRNA} (Castillejo-Lopez and Haecker, 2005, Bidla et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| partially, with Scer\GAL4^Act.PU (Castillejo-Lopez and Haecker, 2005)
Phenotype Manifest In
	embryonic/larval hemolymph \| third instar larval stage, with Scer\GAL4^lz-gal4 (Bidla et al., 2007)
Detailed Description
	Statement Reference Expression of Sp7[dsRNA.Scer\UAS] in crystal cells under the control of Scer\GAL4[lz-gal4] results in reduced hemolymph clot melanization. (Bidla et al., 2007) Flies expressing Sp7^{dsRNA.Scer\UAS} under the control of Scer\GAL4^tub show a severe reduction in the amount of melanisation produced at a wound site (flies were pricked with a needle dipped in a concentrated mixture of E.coli and M.luteus) compared to wild-type flies. Spontaneous melanisation of the hemolymph (assayed by dissecting larvae and exposing them to air) is much lower in flies expressing Sp7^{dsRNA.Scer\UAS} under the control of Scer\GAL4^unspecified larvae than in wild type. (Castillejo-Lopez and Haecker, 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Sp7 gene. (Castillejo-Lopez and Haecker, 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Sp7^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Bidla et al., 2007, J. Cell Sci. 120(7): 1209--1215 Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger. [FBrf0192653] Castillejo-Lopez and Haecker, 2005, Biochem. Biophys. Res. Commun. 338(2): 1075--1082 The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila. [FBrf0189827]

Allele Dmel\Sp7dsRNA.Scer\UAS

Allele Dmel\Sp7^{dsRNA.Scer\UAS}