Allele Dmel\Zfrp8^M-1-1

General Information
Symbol	Dmel\Zfrp8^M-1-1	Species	D. melanogaster
Name		FlyBase ID	FBal0193290
Feature type	allele	Associated gene	Dmel\Zfrp8

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Minakhina et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:20,022,535..20,022,535 na_change=G20022535A pr_change=E296K \| Zfrp8-PA reported_pr_change=E296K comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Minakhina et al., 2007)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: E296K. (Minakhina et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	hyperplasia (Minakhina et al., 2007) hyperplasia (with Df(2R)SM183) (Minakhina et al., 2007) hyperplasia (with Zfrp8^SM206) (Minakhina et al., 2007) lethal (with Df(2R)SM183) (Minakhina et al., 2007) lethal (with Zfrp8^SM206) (Minakhina et al., 2007) melanotic necrosis (Minakhina et al., 2007)
Phenotype Manifest In
	embryonic/larval hemocyte (Minakhina et al., 2007) embryonic/larval lymph gland (Minakhina et al., 2007) melanotic mass (Minakhina and Steward, 2006, Minakhina et al., 2007) melanotic mass (with Df(2R)SM183) (Minakhina and Steward, 2006)
Detailed Description
	Statement Reference More than 90% of hemizygous and homozygous late third instar larvae produce melanotic masses that are seen as black spots outlining the shape of the lymph glands in pupae. Approximately 5% of homozygotes survive to adulthood. The number of circulating hemocytes in mutant third instar larvae is increased about six times compared to wild type. Homozygous late third larval instar lymph glands show an increase in the mitotic index compared to controls. The increase in mitotic cells is concentrated in the secondary lymph gland lobes. (Minakhina et al., 2007) Homozygous and Zfrp8^M-1-1/Df(2R)SM183 mutant third instar larvae have melanotic nodules found in the lymph glands. In Zfrp8^M-1-1/Df(2R)SM183 third instar larvae, some melanotic nodules are surrounded by lamellocytes and some are surrounded by prohemocytes. (Minakhina and Steward, 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Zfrp8^M-1-1/Df(2R)SM183 is partially rescued by Zfrp8^{Scer\UAS.P\T.cMa}/Scer\GAL4^arm.PS (Minakhina et al., 2007) Zfrp8^M-1-1/Zfrp8^SM206 is partially rescued by Zfrp8^{Scer\UAS.P\T.cMa}/Scer\GAL4^arm.PS (Minakhina et al., 2007)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Zfrp8^M-1-1 (Minakhina et al., 2007) zfrp8^M-1-1 (Minakhina and Steward, 2006)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Minakhina et al., 2007, Development 134(13): 2387--2396 Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation. [FBrf0201958] Minakhina and Steward, 2006, Genetics 174(1): 253--263 Melanotic mutants in Drosophila: pathways and phenotypes. [FBrf0194475]

Allele Dmel\Zfrp8M-1-1

Allele Dmel\Zfrp8^M-1-1