Allele Dmel\nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0193316
Feature type	allele	Associated gene	Dmel\nkd
Also Known As	Nkd^{Δ30aaNLS/GFPC}

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Waldrop et al., 2006) in vitro construct - regulatory fusion (Waldrop et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of nkd in which amino acid residues 543-572 have been replaced with the T:SV40\nls2 nuclear localisation signal and which is tagged at the C-terminal end with T:Avic\GFP^EGFP. (Waldrop et al., 2006)
Carried in construct	P{UAS-nkd.Δ30aaNLS.GFPC} (Waldrop et al., 2006, Chan et al., 2008)
Tagged with	T:Avic\GFP^EGFP (Waldrop et al., 2006) T:SV40\nls2 (Waldrop et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Scer\GAL4^da.G32/nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP} partially rescues nkd³ (Waldrop et al., 2006, Chan et al., 2008)
Comments	Expression of nkd[Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP] under the control of Scer\GAL4[da.G32] partially rescues the nkd[3] embryonic cuticle phenotype. (Chan et al., 2008) Expression of nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues the cuticle defects of nkd³ embryos. (Waldrop et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP} Nkd^{Δ30aaNLS/GFPC} (Waldrop et al., 2006, Chan et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Chan et al., 2008, Dev. Biol. 318(1): 17--28 Drosophila Naked cuticle (Nkd) engages the nuclear import adaptor Importin-alpha3 to antagonize Wnt/beta-catenin signaling. [FBrf0205127] Waldrop et al., 2006, Genetics 174(1): 331--348 An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist naked cuticle. [FBrf0192436]

Allele Dmel\nkdΔ30aa.T:SV40\nls2,T:Avic\GFP-EGFP

Allele Dmel\nkd^{Δ30aa.T:SV40\nls2,T:Avic\GFP-EGFP}