Allele Dmel\nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0193323
Feature type	allele	Associated gene	Dmel\nkd::Mmus\Nkd1
Also Known As	mNkd1^f30aa/GFPC

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Waldrop et al., 2006) in vitro construct - regulatory fusion (Waldrop et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Mmus\Nkd1 in which amino acid residues 248-274 have been replaced with amino acid residues 543-572 of nkd. The fusion protein is tagged at the C-terminal end with T:Avic\GFP^EGFP. (Waldrop et al., 2006)
Carried in construct	P{UAS-nkd::Mmus\Nkd1.f30aa.GFPC} (Waldrop et al., 2006, Chan et al., 2007)
Tagged with	T:Avic\GFP^EGFP (Waldrop et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Scer\GAL4^B119, nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}, wg^l-12/wg[+] has visible phenotype (Waldrop et al., 2006)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} is a non-suppressor of denticle belt phenotype of nkd³ (Chan et al., 2007) Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} is a non-suppressor of embryonic/first instar larval cuticle phenotype of nkd³ (Chan et al., 2007) Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} is a non-suppressor of embryonic/first instar larval cuticle phenotype of nkd³/nkd² (Waldrop et al., 2006)
Other
Statement Reference Scer\GAL4^B119, nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}, wg^l-12/wg[+] has sternite phenotype (Waldrop et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of nkd::Mmus\Nkd1[f30aa.Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[da.G32] does not rescue the nkd[3] cuticle phenotype. (Chan et al., 2007) Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 fails to rescue the cuticle defects of nkd²/nkd³ embryos. Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^B119 in a wg^l-12/+ background results in a reduction in the number of sternite bristles on the abdomen. (Waldrop et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	mNkd1^f30aa/GFPC (Waldrop et al., 2006, Chan et al., 2007) nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Chan et al., 2007, Dev. Biol. 311(2): 538--553 Cell-autonomous, myristyl-independent activity of the Drosophila Wnt/Wingless antagonist Naked cuticle (Nkd). [FBrf0201330] Waldrop et al., 2006, Genetics 174(1): 331--348 An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist naked cuticle. [FBrf0192436]

Allele Dmel\nkd::Mmus\Nkd1f30aa.Scer\UAS.T:Avic\GFP-EGFP

Allele Dmel\nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP}