Allele Dmel\tra^F.U2af50

General Information
Symbol	Dmel\tra^F.U2af50	Species	D. melanogaster
Name		FlyBase ID	FBal0193397
Feature type	allele	Associated gene	Dmel\tra

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Evans and Cline, 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Contains 4.5kb of U2af50 upstream genomic sequence, followed by the 216bp U2af50 5' UTR with an improved Cavener translation initiation sequence, followed by the tripeptide MAS and the full female-specific tra open reading frame, followed by the 77bp U2af50 3' UTR and adjacent 1.5kb downstream genomic sequence. (Evans and Cline, 2007)
Carried in construct	P{U2af50-tra^F} (Evans and Cline, 2007, Yang et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference The XY germline degenerates in XY animals expressing tra[F.U2af50]. (Yang et al., 2012) In mosaic XX/XO animals (gynanders) which are carrying tra^F.U2af50, where all the somatic cells of both gonads (those cells inside of the epithelial sheath that surrounds each ovariole) are XO, then the XO somatic cells support normal oogenesis of XX germ cells, resulting in morphologically wild-type eggs. 33% of these gynanders lay their eggs and all those that lay eggs are fertile. In mosaic XX/XO animals (gynanders) which are carrying tra^F.U2af50, XO germ cells form tumorous egg chambers, both when the somatic cells of the gonad (those cells inside of the epithelial sheath that surrounds each ovariole) are XO and also when the somatic cells of the gonad are XX. (Evans and Cline, 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Phf7[ΔN2] significantly rescues germ cell number and organisation in XY animals expressing tra[F.U2af50]. (Yang et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	tra^F.U2af50 rescues tra[-] (Evans and Cline, 2007) tra^F.U2af50 rescues tra¹/Df(3L)st-j7 (Evans and Cline, 2007)
Comments	XX tra¹/Df(3L)st-j7 individuals are feminised by tra^F.U2af50 and the rescued females are fertile. XX tra^- individuals derived from a cross of tra¹/Df(3L)st-j7 females to tra¹/TM6 males are feminised by tra^F.U2af50 and the rescued females are fertile. XX tra^- individuals derived from a cross of tra¹/Df(3L)st-j7 females to tra¹/TM6 males are feminised by tra^F.U2af50 and the rescued females are fertile. XX tra^- individuals derived from a cross of tra¹/Df(3L)st-j7 females to tra⁵/TM6 males are feminised by tra^F.U2af50 and the rescued females are fertile. (Evans and Cline, 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	tra^F.U2af50
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Yang et al., 2012, Dev. Cell 22(5): 1041--1051 Phf7 controls male sex determination in the Drosophila germline. [FBrf0218344] Evans and Cline, 2007, Genetics 175(2): 631--642 Drosophila melanogaster male somatic cells feminized solely by TraF can collaborate with female germ cells to make functional eggs. [FBrf0194910]

Allele Dmel\traF.U2af50

Allele Dmel\tra^F.U2af50