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General Information
Symbol
Dmel\BuffyH37
Species
D. melanogaster
Name
FlyBase ID
FBal0193428
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 1.0kb deletion that includes almost the entire Buffy coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A significant increase in the survival of glial cells within notal microchaete clusters is seen in BuffyH37 homozygotes.

Mutants are viable with no overt mutant adult phenotypes.

The distribution and number of apoptotic cells is similar to that of control embryos in mutant stage 11 and late stage 12 embryos.

Stage 16 mutant embryos have normal central and peripheral nervous system patterning and no fusion of commissural axon bundles is seen.

Normal histolysis of the larval midgut occurs during pupation in the mutant animals and larval salivary glands also undergo histolysis. Programmed cell death in the pupal retina also occurs normally to remove a subset of interommatidial cells. Programmed cell death in the arista is also normal in the mutants.

The pattern of mitotic cells in the developing mutant embryo is normal.

The normal random pattern of mitotic cells seen in third larval instar wing imaginal discs is not altered in BuffyH37 mutants, and cells in these wing discs arrest normally following irradiation.

Mutant adults are not susceptible to infection by E.coli (infection by pricking with a needle dipped in a concentrated pellet of E.coli).

The apoptotic response to irradiation is slightly increased in mutant embryos compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference

Buffy[+]/BuffyH37 is a non-enhancer of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], DebclE26, BuffyH37 is a non-enhancer of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], BuffyH37, DebclW105 is a non-enhancer of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Buffy[+]/BuffyH37 is a non-suppressor of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], DebclE26, BuffyH37 is a non-suppressor of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], BuffyH37, DebclW105 is a non-suppressor of visible phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Other
Statement
Reference
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

Buffy[+]/BuffyH37 is a non-enhancer of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], DebclE26, BuffyH37 is a non-enhancer of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], BuffyH37, DebclW105 is a non-enhancer of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Suppressor of
Statement
Reference

BuffyH37 is a suppressor | partially of eye phenotype of grimGMR.PC

NOT Suppressor of
Statement
Reference

Buffy[+]/BuffyH37 is a non-suppressor of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], DebclE26, BuffyH37 is a non-suppressor of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Buffy[+], Debcl[+], BuffyH37, DebclW105 is a non-suppressor of eye phenotype of Hsap\BCL2L13UAS.cNa, Scer\GAL4GMR.PS

Additional Comments
Genetic Interactions
Statement
Reference

The cell death seen in grimGMR.PC eyes is abrogated in a BuffyH37 background.

debclE26 BuffyH37 double mutants are viable with no overt mutant adult phenotypes.

The distribution and number of apoptotic cells is similar to that of control embryos in debclE26 BuffyH37 double mutant stage 11 and late stage 12 embryos. Stage 16 double mutant embryos have normal central and peripheral nervous system patterning and no fusion of commissural axon bundles is seen. Normal histolysis of the larval midgut occurs during pupation in the double mutant animals and larval salivary glands also undergo histolysis. Programmed cell death in the pupal retina also occurs normally to remove a subset of interommatidial cells. Programmed cell death in the arista is also normal in the double mutants.

The pattern of mitotic cells in the developing debclE26 BuffyH37 double mutant embryo is normal.

debclE26 BuffyH37 double adults are not susceptible to infection by E.coli (infection by pricking with a needle dipped in a concentrated pellet of E.coli).

The apoptotic response to irradiation is increased in debclE26 BuffyH37 and debclW105 BuffyH37 double mutant embryos compared to wild type.

Xenogenetic Interactions
Statement
Reference

The rough eye phenotype induced by expression of Hsap\BCL2L13Scer\UAS.cNa driven by Scer\GAL4GMR.PS is not significantly enhanced or suppressed by DebclE26 BuffyH37/+, DebclW105 BuffyH37/+ or BuffyH37/+ mutations. DebclE26 BuffyH37/+, DebclW105 BuffyH37/+ or BuffyH37/+ in Scer\GAL4GMR.PS>Hsap\BCL2L13ΔTM.Scer\UAS flies does not result in significant eye phenotypes.

Heterozygosity for BuffyH37 does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4GMR.PU, leading to severe eye necrosis.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)