Allele Dmel\GLaz^Scer\UAS.cWa

General Information
Symbol	Dmel\GLaz^Scer\UAS.cWa	Species	D. melanogaster
Name		FlyBase ID	FBal0193460
Feature type	allele	Associated gene	Dmel\GLaz

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Walker et al., 2006, Walker, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Five copies of Scer\UAS regulatory sequences drive expression of GLaz coding sequences. (Walker et al., 2006, Walker, 2006)
Carried in construct	P{UAS-Glaz.W} (Walker et al., 2006, Walker, 2006)
Cytology
Phenotypic Data
Phenotypic Class
	hyperactive, with Scer\GAL4^109(2)80 (Walker et al., 2006) long lived, with Scer\GAL4^109(2)80 (Walker et al., 2006, Walker, 2006) long lived \| heat sensitive, with Scer\GAL4^12.1 (Walker et al., 2006) long lived \| heat sensitive, with Scer\GAL4^109(2)80 (Walker et al., 2006) long lived \| heat sensitive, with Scer\GAL4^GMR.PF (Walker et al., 2006) long lived \| heat sensitive, with Scer\GAL4^how-24B (Walker et al., 2006) long lived \| heat sensitive, with Scer\GAL4^Lk6-DJ634 (Walker et al., 2006) stress response defective, with Scer\GAL4^109(2)80 (Walker et al., 2006, Walker, 2006) stress response defective, with Scer\GAL4^da.G32 (Walker et al., 2006)
Phenotype Manifest In

Detailed Description
	Statement Reference Expression of GLaz^Scer\UAS.cWa, under the control of Scer\GAL4^D42, Scer\GAL4^Act5C, Scer\GAL4^hs.PB or Scer\GAL4^elav-C155 at 29^oC does not have any significant effect on lifespan. Expression of GLaz^Scer\UAS.cWa in the central nervous system and some muscles, under the control of Scer\GAL4^12.1 results in an 8% increase in lifespan. Expression of GLaz^Scer\UAS.cWa in the central nervous system and some muscles, under the control of Scer\GAL4^109(2)80 at 29^oC results in an 23% increase in lifespan. Expression of GLaz^Scer\UAS.cWa in the fat and some muscles, under the control of Scer\GAL4^Lk6-DJ634 at 29^oC results in an 16% increase in lifespan. Expression of GLaz^Scer\UAS.cWa in the fat and some muscles, under the control of Scer\GAL4^how-24B at 29^oC results in an 28% increase in lifespan. Expression of GLaz^Scer\UAS.cWa in the eye and salivary glands, under the control of Scer\GAL4^GMR.PF at 29^oC results in an 23% increase in lifespan. Flies carrying both GLaz^Scer\UAS.cWa and Scer\GAL4^109(2)80 at 29^oC display a 12% increase in survival under hyperoxia compared to the Scer\GAL4^109(2)80 driver control. After 4 days in hyperoxia, flies carrying both GLaz^Scer\UAS.cWa and Scer\GAL4^109(2)80 at 29^oC display both vertical and horizontal locomotor performances that are much improved compared to controls. No differences are apparent under normoxia. Overexpression of GLaz^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 leads to a 60% increase in lifespan under wet starvation and a 30% increase under dry starvation. There is no significant difference in dry weight, protein content, or fat content between GLaz^Scer\UAS.cWa Scer\GAL4^109(2)80 flies and controls. Flies overexpressing GLaz^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 that are subjected to hypoxia in 100% N₂ for 30 minutes, then transferred back to a normal environment are much more active than control flies. One hour after recovery, approximately 75% of the overexpression flies climb up to the top third of the vial, while only 10-15% of control flies manage the same. This difference is still evident 2 days later. There is no difference in climbing ability before hypoxia exposure. (Walker et al., 2006) Overexpression of GLaz^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 leads to a 60% increase in lifespan under wet starvation and a 30% increase under dry starvation. There is no significant difference in dry weight, protein content, or fat content between GLaz^Scer\UAS.cWa Scer\GAL4^109(2)80 flies and controls. (Walker, 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	27905 w^*; P{UAS-Glaz.W}2; SNF4Aγ^loe/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	GLaz^Scer\UAS.cWa
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Walker et al., 2006, Curr. Biol. 16(7): 674--679 Overexpression of a Drosophila homolog of apolipoprotein D leads to increased stress resistance and extended lifespan. [FBrf0190050]
Supplementary material	Walker, 2006, Curr. Biol. 16(7): [FBrf0198586]

Allele Dmel\GLazScer\UAS.cWa

Allele Dmel\GLaz^Scer\UAS.cWa