dysf2/dysf3 escapers display a complete absence of tarsal joint formation with a small shortening of the tarsal region. No defects are observed in most proximal joints or in the tarsus/pretarsus joint. The joint between the a5 and the arista in the antenna is also lost.
The distribution of apoptotic cells in the dysf2/dysf3 mutant prepupal leg is altered compared to wild type.
The ganglionic branches, dorsal branches, and lateral trunk branches in dys2/dys3 mutant second instar larvae show a complete absence of fusion in all branches examined. The dorsal trunk is fused and relatively normal in appearance, although some dorsal trunk fusion sites are constricted. Penetrance and expressivity are 100%.
In dys2/dys3 stage 16 embryos the dorsal branches and lateral trunks fail to fuse, while the dorsal trunk is fused.