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General Information
Symbol
Dmel\DarkUAS.Tag:polyHis,Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0193514
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-darkWT, UAS-ark
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Dark coding sequences with 8xTag:polyHis at the N-terminal end and 6xTag:MYC tags at the carboxy terminal end.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in the developing eye-antennal discs, under the control of Scer\GAL4GMR.PF does not induce significant apoptosis.

Expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in hemocytes, under the control of Scer\GAL4Hml.PG only mildly restores apoptosis after Smac mimetic treatment.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
NOT suppressed by
Enhancer of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Embryos co-expressing Droncpro.Scer\UAS and DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC in neurons under the control of Scer\GAL4elav.PU exhibit increased engulfment of apoptotic neurons.

The combined expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF generates a potent eye ablation phenotype.

Either a BruceE81/+ or BruceE23/+ heterozygous background has no effect on the eye ablation phenotype found upon co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF.

Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS together, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal discs and adult eyes.

Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC, Ncpro.Scer\UAS and hpoScer\UAS.cUa in the developing eye, under the control of Scer\GAL4GMR.PF induces massive apoptosis in the eye-antennal disc and adult. This phenotype appears stronger than controls as both photoreceptor and pigment cells are eventually eliminated in animals co-expressing hpoScer\UAS.cUa.

Co-expression of ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC and Ncpro.Scer\UAS with ykiScer\UAS.cHa, all under the control of Scer\GAL4GMR.PF, completely suppresses the overgrowth phenotype of ykiScer\UAS.cHa overexpression, due to induction of apoptosis in response to increased Nc activity.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ArkScer\UAS.T:Zzzz\His6,T:Hsap\MYC
DarkScer\UAS.T:Zzzz\His6,T:Hsap\MYC
DarkUAS.Tag:polyHis,Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (7)