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General Information
Symbol
Dmel\fondsRNA.IR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193517
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of inverted repeats corresponding to 1-500bp of the fon coding sequence, separated by an intronic DNA sequence that acts as a spacer and results in a hairpin-loop shaped RNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of fondsRNA.IR.Scer\UAS under the control of Scer\GAL4da.PU or Scer\GAL4ppl.PP, but not Scer\GAL4sr-md710, leads to significant muscle detachment in wandering third instar larvae, as compared to controls; expression under the control of Scer\GAL4how-24B has no significant effect on muscle detachment as compared to controls, but leads to occasional muscle loss.

Expression of fondsRNA.IR.Scer\UAS, under the control of Scer\GAL4da.G32, results in larvae that are viable and exhibit no further visible phenotype except the sporadic presence of small melanotic spots. The hemocyte count in fondsRNA.IR.Scer\UAS larvae is normal, but large aggregates of plasmatocyte and lamellocytes are detected in some hemolymph samples. fondsRNA.IR.Scer\UAS pupae display an abnormal shape. Large numbers of pupae are considerably elongated, with many of them showing a bend on the ventral side, causing a banana-shaped appearance. All normally shaped pupae survive into adulthood, whereas banana-shaped ones die as pupae. Approximately 45-50% of the pupae raised at 25oC are elongated compared to wild-type pupae and show an unusual banana-like shape with the dorsal part bent upwards. This seemed to be connected to a defect in body retraction of late larvae prior to pupation , which attached to the vial walls with their anterior part only , while the posterior end was performing circular searching movements in the air. Spiracle eversion seems to be defective in most of these pupae, while the anterior end of the pupae is often heavily melanized . The penetrance of the phenotype increased at 29oC. At this temperature, about 70 to 90 % of the fondsRNA.IR.Scer\UAS pupae are longer and less compact than wild-type pupae. Approximately 50 % of all pupae display the bowed shape associated with pupal lethality.

Expression of fondsRNA.IR.Scer\UAS, under the control of Scer\GAL4ppl.PP results in pupae that display an abnormal shape. Large numbers of pupae are considerably elongated, with many of them showing a bend on the ventral side, causing a banana-shaped appearance. All normally shaped pupae survive into adulthood, whereas banana-shaped ones die as pupae. Approximately 54% of the pupae raised at 25oC are elongated compared to wild-type pupae and show an unusual banana-like shape with the dorsal part bent upwards. This seemed to be connected to a defect in body retraction of late larvae prior to pupation , which attached to the vial walls with their anterior part only , while the posterior end was performing circular searching movements in the air. Spiracle eversion seems to be defective in most of these pupae, while the anterior end of the pupae is often heavily melanized . The penetrance of the phenotype increased at 29oC. At this temperature, about 70 to 90 % of the fondsRNA.IR.Scer\UAS pupae are longer and less compact than wild-type pupae. Approximately 50 % of all pupae display the bowed shape associated with pupal lethality.

Pupae expressing fondsRNA.IR.Scer\UAS, under the control of Scer\GAL4ppl.PP exhibit fragile cuticles.

Fibers from fondsRNA.IR.Scer\UAS-treated hemolymph (where fondsRNA.IR.Scer\UAS is under the control of Scer\GAL4da.G32) are less frequent and appear less compact and thinner compared to normal hemolymph.

The average size of the melanized spot in larvae treated with fondsRNA.IR.Scer\UAS (under the control of Scer\GAL4da.G32) is bigger than in controls. Melanization spreads more widely from the wound site over the cuticle, while the central part of the wound is often insufficiently melanized. fondsRNA.IR.Scer\UAS-treated larvae do not exhibit a higher mortality rate compared to controls.

The survival rate of fondsRNA.IR.Scer\UAS (under the control of Scer\GAL4da.G32) flies is significantly weaker towards Gram-negative infections but is not different from controls in other types of infection.

Parasitization of flies treated with fondsRNA.IR.Scer\UAS (under the control of Scer\GAL4da.G32) by the wasp Leptopilina boulardii generates a lamellocyte response and melanotic spots similar to that found in wild-type controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of fondsRNA.IR.Scer\UAS and HmldsRNA.Scer\UAS, under the control of Scer\GAL4da.G32 in larvae does not generate any additional phenotype compared to the single fondsRNA.IR.Scer\UAS mutant alone. Clot fibers from fondsRNA.IR.Scer\UAS-treated larvae are more ductile and longer than controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
fondsRNA.IR.Scer\UAS
fondsRNA.IR.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)