FB2025_01 , released February 20, 2025
Allele: Dmel\delUASp.Tag:HA
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General Information
Symbol
Dmel\delUASp.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0193531
Feature type
allele
Associated gene
Dmel\del
Associated Insertion(s)
Carried in Construct
P{UASp-del-HA}
Key Links
Transgenic product class
cDNA
(Wehr et al., 2006)
wild_type
(Wehr et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Wehr et al., 2006)
wild_type
(Wehr et al., 2006)
Mutagen
in vitro construct
(Wehr et al., 2006)
Progenitor genotype
Carried in construct
P{UASp-del-HA}
Cytology
Description

UASp regulatory sequences drive expression of del cDNA (derived from the SD07269 EST clone) which have two copies of Tag:HA in frame at the 5' end.

(Wehr et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
del
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Eggs laid by del2/del4 females expressing delScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 do not develop very far. Most eggs exhibit a mild ventralization with two closely spaced appendages or one single appendage indicating a partial rescue of later phenotypes. Expression of delScer\UAS.T:Ivir\HA1 under the control of the maternal Scer\GAL4mat.αTub67C.T:Hsim\VP16 restores oogenesis, although the females lay few eggs which do not develop very far.

      The majority of embryos from del2/del4 mutants that express delScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 are arrested in the metaphase of the first mitotic division.

      (Wehr et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      One copy of delScer\UAS.T:Ivir\HA1, expressed under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 restores egg chamber production in del2/del4 mutants, resulting on ovaries of wild-type size. Ovarioles in rescued females contain normal numbers of developing egg chambers that lack defects in encapsulation and oocyte positioning. karyosome formation is restored as well.

      Expression of delScer\UAS.T:Ivir\HA1 in del2/del4 mutants, under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 renders males fertile.

      (Wehr et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      delScer\UAS.T:Ivir\HA1
      delUASp.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)