Allele Dmel\CadN^Δ14

General Information
Symbol	Dmel\CadN^Δ14	Species	D. melanogaster
Name		FlyBase ID	FBal0193539
Feature type	allele	Associated gene	Dmel\CadN
Also Known As	Ncad^Δ14

Allele class
Mutagen	X ray
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	X ray (Prakash et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{GT1}BG01221 (Prakash et al., 2005)
Nature of the lesion	Statement Reference X-ray-mediated deletion of P{GT1}BG01221, resulting in deletion of the entire CadN2 gene, part of the CadN gene and sequences in between the two genes (including the P{GT1}BG01221 element). (Prakash et al., 2005)
Caused by aberration	Df(2L)CadN^Δ14 (Prakash et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Choe et al., 2006, Nern et al., 2008) neuroanatomy defective \| pupal stage \| somatic clone (Prakash et al., 2009) neuroanatomy defective \| somatic clone (Tong et al., 2011)
Phenotype Manifest In
	eye photoreceptor cell (Choe et al., 2006) lamina (Choe et al., 2006) lamina monopolar neuron (Nern et al., 2008) medulla (Choe et al., 2006) optic cartridge (Choe et al., 2006) photoreceptor cell \| pupal stage \| somatic clone (Prakash et al., 2009) photoreceptor cell R4 \| cell non-autonomous \| somatic clone (Choe et al., 2006) photoreceptor cell R4 \| pupal stage \| somatic clone (Prakash et al., 2009) photoreceptor cell R7 (Nern et al., 2008) photoreceptor cell R7 \| somatic clone (Tong et al., 2011)
Detailed Description
	Statement Reference chp staining of the medulla of CadN[Δ14] eye clones (made using the 'eyFLP' system) reveals a few 'gaps' as if some R7 terminals are missing. CadN[Δ14] eye clones (made using the 'eyFLP' system) have R7 targeting phenotypes. (Tong et al., 2011) When large clones of CadN[Δ14] mutant cells are generated in the eye, R4 axons frequently fail to extend, or extend aberrantly. (Prakash et al., 2009) Using MARCM, mistargeting of CadN[Δ14] mutant L1-L5 cells is seen at the following percentages: 29% of L1 neurons, typically terminating in M10 rather than M5; 0% of L2 neurons; 81% of L3 neuron, typically terminating in M5 and M6 rather than M3; 81% of L4 neurons, terminating in M2 or, less frequently, M8 instead of M4; 0% of L5 terminals, but 100% of L5 interstitial branches fail to extend from M1 into M2. 100% of CadN[Δ14] mutant R7 cells mistarget. 4% of R8 cells extend significantly deeper into the medulla than the remaining R8 cells, similar to controls. At 25hrs APF, the centres of CadN[Δ14] L3 growth cones are located in the R7 temporary layer about 2υm more proximal than in wild-type L3s. Mutant L3 terminals continue to exhibit incorrect layer choice, and remain elongated along one side of the column with only some diffuse lateral processes. When using a reverse MARCM approach, 44/825 wildtype L5 neurons, neighboured by mutant cells, are observed in which almost no interstitial branches extend into M2 but instead send branches into adjacent columns, compared to controls which show no targeting defects. (Nern et al., 2008) CadN^Δ14 mutants exhibit a specific pattern of disruption in the structure of the cartridge, the synaptic unit in the lamina. Some cartridges have either >6 or <6 R cells axons, and some adjacent cartridges fuse. In CadN^Δ14 mutants, R7 axons frequently stop at abnormally distal positions within the R8 recipient layer, leaving gaps in the array of otherwise regular R7 termini. However the ganglion-specific targeting of R1-R6 axons to the lamina nor the layer-specific targeting of R8 axons within the medulla are affected. In CadN^Δ14 homozygous mutants, the pattern of cartridges (where the R cell axons and lamina neuron processes form a highly organised fascicle) is severely disrupted. In CadN^Δ14 mutant target clones, the array of R4 axons appear disrupted, with many axons failing to extend, while others target inappropriately. (Choe et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement Reference CadN^Δ14 has neuroanatomy defective \| somatic clone \| pupal stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^Scer\UAS.cKa (Prakash et al., 2009)
Enhancer of
Statement Reference CadN[+]/CadN^Δ14 is an enhancer of lethal phenotype of Lar²¹²⁷/Lar⁴⁵¹ (Prakash et al., 2009)
NOT Enhancer of
Statement Reference CadN[+]/CadN^Δ14 is a non-enhancer of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011)
Suppressor of
Statement Reference CadN[+]/CadN^Δ14 is a suppressor \| partially of lethal phenotype of Liprin-α^E/Liprin-α¹ (Prakash et al., 2009) CadN[+]/CadN^Δ14 is a suppressor \| partially of lethal phenotype of Liprin-α^F/Liprin-α^E (Prakash et al., 2009)
NOT Suppressor of
Statement Reference CadN[+]/CadN^Δ14 is a non-suppressor of planar polarity defective phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011)
Other
Statement Reference CadN[+]/CadN^Δ14, Lar²¹²⁷/Lar[+], Liprin-α^F has partially lethal - majority die \| dominant phenotype (Prakash et al., 2009) CadN^Δ14, Rich¹ has neuroanatomy defective \| somatic clone phenotype (Tong et al., 2011)
Phenotype Manifest In
NOT suppressed by
Statement Reference CadN^Δ14 has photoreceptor cell \| somatic clone \| pupal stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^Scer\UAS.cKa (Prakash et al., 2009)
NOT Enhancer of
Statement Reference CadN[+]/CadN^Δ14 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011)
NOT Suppressor of
Statement Reference CadN[+]/CadN^Δ14 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^Scer\UAS.cUa (Mirkovic et al., 2011) CadN^Δ14 is a non-suppressor of ommatidium phenotype of ec^spok (Montrasio et al., 2007)
Other
Statement Reference CadN^Δ14, Rich¹ has photoreceptor cell R7 \| somatic clone phenotype (Tong et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference CadN[Δ14], Rich[1] double mutant eye clones (made using the 'eyFLP' system) have R7 targeting phenotypes similar to CadN[Δ14] single mutants. (Tong et al., 2011) When large clones of cells mutant for both Liprin-α[E] and CadN[Δ14] are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants. When large clones of cells mutant for both Lar[2127] and CadN[Δ14] are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants. Expression of Lar[Scer\UAS.cKa] under the control of Scer\GAL4[elav-C155] in CadN[Δ14] somatic mutant clones does not rescue the photoreceptor targeting defects. When large clones of cells mutant for Liprin-α[E], Lar[2127], and CadN[Δ14] are generated in the eye, R4 axon targeting errors are observed, but the frequency of defects is similar to Liprin-α[E]; Lar[2127], Lar[2127]; CadN[Δ14], or Liprin-α[E]; CadN[Δ14] double mutant combinations. (Prakash et al., 2009) The ommatidial rotation defects observed in ec[spok] hemizygous adult eyes are not suppressed by CadN[Δ14]/+. (Montrasio et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CadN^Δ14 (Nern et al., 2008, Tong et al., 2011) Ncad^Δ14 (Mirkovic et al., 2011, Montrasio et al., 2007) ncad^Δ14 (Gontang et al., 2011) N-cadherin^Δ14 (Prakash et al., 2009) Δ14 (Prakash et al., 2005)
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Gontang et al., 2011, Development 138(22): 4899--4909 The cytoskeletal regulator Genghis khan is required for columnar target specificity in the Drosophila visual system. [FBrf0216508] Tong et al., 2011, Neuron 71(3): 447--459 Rich Regulates Target Specificity of Photoreceptor Cells and N-Cadherin Trafficking in the Drosophila Visual System via Rab6. [FBrf0214681] Prakash et al., 2009, Dev. Biol. 336(1): 10--19 Complex interactions amongst N-cadherin, DLAR, and Liprin-alpha regulate Drosophila photoreceptor axon targeting. [FBrf0209258] Nern et al., 2008, Neuron 58(1): 34--41 Local N-cadherin interactions mediate distinct steps in the targeting of lamina neurons. [FBrf0204173] Montrasio et al., 2007, Dev. Dyn. 236(10): 2936--2942 A new allele uncovers the role of echinus in the control of ommatidial rotation in the Drosophila eye. [FBrf0202297] Choe et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(31): 11601--11606 Liprin-alpha is required for photoreceptor target selection in Drosophila. [FBrf0195369] Prakash et al., 2005, Nat. Neurosci. 8(4): 443--450 Drosophila N-cadherin mediates an attractive interaction between photoreceptor axons and their targets. [FBrf0184200]
Supplementary material	Mirkovic et al., 2011, Nat. Struct. Mol. Biol. 18(6): Supplemental Data. [FBrf0213975]

Allele Dmel\CadNΔ14

Allele Dmel\CadN^Δ14