The ft coding fragment has an internal deletion, that starts at QPT137 and ends at C4569. This begins with the first cadherin domain and ends three amino acids into the transmembrane domain.
Ectopic expression of ftΔECD.Scer\UAS under the control of Scer\GAL4nub.PU results in severely undergrown wings in adult flies.
Expression of ftΔECD.Scer\UAS under the control of Scer\GAL432B produces flies with reduced wing size compared to controls.
Expression of ftΔECD.Scer\UAS under the control of Scer\GAL4Act5C.PI causes mild perturbation of planar cell polarity in the wing and a foreshortening along the proximodistal axis of the wing blade as the anterior and posterior crossveins are abnormally close.
Overexpression of ftΔECD.Scer\UAS in the developing wing, under the control of Scer\GAL4nub-AC-62 causes small and narrow wings.
Scer\GAL4nub.PU, ftΔECD.UAS has visible | adult stage phenotype, suppressible | partially by Scer\GAL4nub.PU/ZyxUAS.cGa
Scer\GAL4nub.PU, ftΔECD.UAS has hypoplasia phenotype, suppressible | partially by Scer\GAL4nub.PU/ZyxUAS.cGa
Scer\GAL432B, ftΔECD.UAS has visible phenotype, suppressible by Tao50
Scer\GAL432B, ftΔECD.UAS has size defective phenotype, suppressible by Tao50
Scer\GAL4nub.PU, ftΔECD.UAS has wing blade phenotype, suppressible | partially by Scer\GAL4nub.PU/ZyxUAS.cGa
Scer\GAL432B, ftΔECD.UAS has wing phenotype, suppressible by Tao50
Scer\GAL4nub-AC-62, ftΔECD.UAS has wing phenotype, suppressible by wtsx1/wts[+]
The severely undergrown wing phenotype seen in adult flies expressing ftΔECD.Scer\UAS under the control of Scer\GAL4nub.PU can be mildly alleviated by co-expression of ZyxScer\UAS.cGa.
Tao50 suppresses the reduced wing size phenotype seen when ftΔECD.Scer\UAS is expressed under the control of Scer\GAL432B.
S2 cells that express with dsΔECD.Scer\UAS and ftΔECD.Scer\UAS, under the control of Scer\GAL4Act5C.PI, fail to aggregate.
The ftΔECD.Scer\UAS phenotype (under the control of Scer\GAL4nub-AC-62) is suppressed by wtsx1 heterozygosity.
Scer\GAL4en-e16E/ftΔECD.UAS partially rescues ft8/ftG-rv
Scer\GAL4Act5C.PI, Scer\GAL4Act5C.PI, ftΔECD.UAS partially rescues ft8/ftG-rv
Scer\GAL4da.G32/ftΔECD.UAS partially rescues ft8/ftG-rv
Expression of ftΔECD.Scer\UAS, under the control of either Scer\GAL4Act5C.PI or Scer\GAL4da.G32, rescues the lethality of ftG-rv/ft8 mutants and is effective in rescuing the wing disc overgrowth phenotype. Expression of ftΔECD.Scer\UAS in the posterior wing pouch, driven by Scer\GAL4en-e16E, rescues the overgrowth phenotype of ftG-rv/ft8 mutants in this region, but does not rescue overgrowth in the anterior.
Expression of ftΔECD.Scer\UAS under the control of Scer\GAL4Act5C.PI in ftG-rv/ft8 mutants partially rescues the abdominal planar cell polarity defect.
Carried in a plasmid and transfected into S2 cells for use in cell aggregation assays.