Allele Dmel\d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}

General Information
Symbol	Dmel\d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}	Species	D. melanogaster
Name		FlyBase ID	FBal0193610
Feature type	allele	Associated gene	Dmel\d

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Mao et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	d^Scer\UAS.cMa (Mao et al., 2006)
Nature of the lesion	Statement Reference T:SV5\V5 and T:Zzzz\His6 epitopes have replaced the stop codon and 3' UTR of d. (Mao et al., 2006)
Carried in construct	P{UAS-d.V5} (Mao et al., 2006, Poon et al., 2011, Matakatsu and Blair, 2008, Feng and Irvine, 2009, Rauskolb et al., 2011)
Tagged with	T:SV5\V5 (Mao et al., 2006) T:Zzzz\His6 (Mao et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	size defective, with Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011) size defective \| adult stage, with Scer\GAL4^en-e16E (Poon et al., 2011) visible, with Scer\GAL4^tub.PU (Mao et al., 2006) visible \| adult stage, with Scer\GAL4^en-e16E (Poon et al., 2011)
Phenotype Manifest In
	crossvein, with Scer\GAL4^tub.PU (Mao et al., 2006) leg, with Scer\GAL4^tub.PU (Mao et al., 2006) wing, with Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011) wing, with Scer\GAL4^tub.PU (Mao et al., 2006) wing \| posterior, with Scer\GAL4^en-e16E (Poon et al., 2011)
Detailed Description
	Statement Reference Expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] under the control of Scer\GAL4[en-e16E] results in wing overgrowth. (Poon et al., 2011) Expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] (together with Dcr-2[Scer\UAS.cDa]) using Scer\GAL4[nub-AC-62] results in overgrown wings. (Rauskolb et al., 2011) Expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] under the control of Scer\GAL4[tub] has no effect on planar cell polarity in the adult abdomen. (Matakatsu and Blair, 2008) Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub results in increased wing size and crossvein abnormalities and also induces tissue outgrowth in the leg. (Mao et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^nub-AC-62, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has size defective phenotype, enhanceable by Scer\GAL4^nub-AC-62/Zyx^{Scer\UAS.T:SV5\V5} (Rauskolb et al., 2011)
Suppressed by
Statement Reference Scer\GAL4^en-e16E, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has size defective \| adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} (Poon et al., 2011) Scer\GAL4^en-e16E, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has visible \| adult stage phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} (Poon et al., 2011) Scer\GAL4^nub-AC-62, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has size defective phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Enhancer of
Statement Reference Scer\GAL4^nub-AC-62/d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} is an enhancer of size defective phenotype of Scer\GAL4^nub-AC-62, Zyx^{Scer\UAS.T:SV5\V5} (Rauskolb et al., 2011)
Suppressor of
Statement Reference Scer\GAL4^ap-md544/d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} is a suppressor of planar polarity defective phenotype of app^e6 (Matakatsu and Blair, 2008)
Other
Statement Reference Scer\GAL4^tub.PU, app^RA.Scer\UAS, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has planar polarity defective phenotype (Matakatsu and Blair, 2008)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^nub-AC-62, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has wing phenotype, enhanceable by Scer\GAL4^nub-AC-62/Zyx^{Scer\UAS.T:SV5\V5} (Rauskolb et al., 2011)
Suppressed by
Statement Reference Scer\GAL4^en-e16E, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has wing \| posterior phenotype, suppressible by Scer\GAL4^en-e16E/Tao^{Scer\UAS.P\T.T:Zzzz\FLAG} (Poon et al., 2011) Scer\GAL4^nub-AC-62, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has wing phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Enhancer of
Statement Reference Scer\GAL4^nub-AC-62/d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, Zyx^{Scer\UAS.T:SV5\V5} (Rauskolb et al., 2011)
Other
Statement Reference Scer\GAL4^tub.PU, app^RA.Scer\UAS, d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} has adult abdomen phenotype (Matakatsu and Blair, 2008)
Additional Comments
Genetic Interactions
Statement Reference Expression of Tao[Scer\UAS.P\T.T:Zzzz\FLAG] reduces the wing overgrowth seen when d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] is expressed under the control of Scer\GAL4[en-e16E]. (Poon et al., 2011) The wing overgrowth phenotype associated with Scer\GAL4[nub-AC-62]-mediated expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] (together with Dcr-2[Scer\UAS.cDa]) is suppressed by co-expression of Zyx102EF[NIG.32018R]. Over-expression of Zyx102EF[Scer\UAS.T:SV5\V5] (together with Dcr-2[Scer\UAS.cDa]) using Scer\GAL4[nub-AC-62] synergizes with d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] over-expression to result in enhanced wing overgrowth in adult flies. (Rauskolb et al., 2011) Co-expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] and app[RA.Scer\UAS] under the control of Scer\GAL4[tub] results in planar cell polarity defects in the adult abdomen. The PCP defects that are seen between distal veins L4 and L5 in app[e6] mutants are rescued on the dorsal surface by expression of d[Scer\UAS.T:SV5\V5,T:Zzzz\His6] under the control of Scer\GAL4[ap-md544]. (Matakatsu and Blair, 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}/Scer\GAL4^tub.PU partially rescues d^GC13 (Mao et al., 2006)
Comments	Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub provides significant rescue of the wing and leg phenotypes of d^GC13 mutants. (Mao et al., 2006)
Stocks ( 2 )
Bloomington	28291 w^; P{UAS-d.V5}9-F 28814 w^; P{UAS-d.V5}50-5/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Poon et al., 2011, Dev. Cell 21(5): 896--906 The Sterile 20-like Kinase Tao-1 Controls Tissue Growth by Regulating the Salvador-Warts-Hippo Pathway. [FBrf0216620] Rauskolb et al., 2011, PLoS Biol. 9(6): e1000624 Zyxin links fat signaling to the hippo pathway. [FBrf0213900] Feng and Irvine, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(29): 11989--11994 Processing and phosphorylation of the Fat receptor. [FBrf0208432] Matakatsu and Blair, 2008, Curr. Biol. 18(18): 1390--1395 The DHHC palmitoyltransferase approximated regulates Fat signaling and Dachs localization and activity. [FBrf0206015] Mao et al., 2006, Development 133(13): 2539--2551 Dachs: an unconventional myosin that functions downstream of Fat to regulate growth, affinity and gene expression in Drosophila. [FBrf0193425]

Allele Dmel\dScer\UAS.T:SV5\V5,T:Zzzz\His6

Allele Dmel\d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6}