Y330term | eys-PE; Y330term | eys-PF
The difference between the reported and annotated positions of the amino acid change in the mutant results from the use of a downstream translation start in the previous eys annotation and the publication. The annotation was updated in T5.55 to start at an in-frame ATG which is 11aa upstream.
One copy of both eys1 and prom1 results in a partial failure to form a continuous open inter-rhabdomeral space (retinal lumen), exhibited by the presence of juxtaposed rhabdomeres. This phenotype is significantly enhanced by one copy of either Df(1)ED6829 or Act5CG0245.
Although the rhabdomere phenotype is recessive in eys1 and prom1 single mutants, eys1/+, prom1/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.
prom1/prom1; chp2/+ double mutant flies show suppression of the prom1/prom1 rhabdomeral phenotype. However, in prom1/prom1, eys1/+; chp2/+ flies the rhabdomeres are stuck together, so the mild prom1/prom1; chp2/+ phenotype is enhanced in prom1/prom1, eys1/+; chp2/+ flies.
In prom1/prom1, eys1/+; chp2/+ triple mutant flies, the rescue of the prom1/prom1 phenotype is reduced compared to the prom1/prom1; chp2/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.
Expression of Hsap\EYSScer\UAS.cNa in the developing eye under the control of Scer\GAL4GMR.PF and Scer\GAL4hs.PU partially and mildly suppresses the eys1 mutant eye phenotype, with only pockets of inter-rhabdomeral space nd many rhabdomeres remaining juxtaposed to each other.
Expression of Hsap\EYSScer\UAS.cNa and Hsap\PROM1Scer\UAS.cNa in eys1, prom1 double mutants results in rescue of the eye phenotype, with separation of the rhabdomeres and the presence of inter-rhabdomeral space.
Expression of eysScer\UAS.cZa under the control of Scer\GAL4hs.PB fully rescues the rhabdomere phenotype of eys1 flies when eysScer\UAS.cZa expression is induced at 36-64 hours after puparium formation. Induction at about 72 hours, using either Scer\GAL4hs.PB or Scer\GAL4ninaE.PD, leads only to a partial rescue.