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General Information
Symbol
Dmel\prom1
Species
D. melanogaster
Name
FlyBase ID
FBal0193674
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Lesion in the prom gene.

    Nucleotide substitution: G764A.

    The mutation affects the acceptor site.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    prom1/prom1 mutant pupae display fusion of rhabdomeres and a reduction in size of the inter-rhabdomeral lumen.

    prom1 mutant ommatidia have fused rhabdomeres.

    prom1 mutant photoreceptor cells undergo light-dependent degeneration. The retinal degeneration can be prevented in the absence of dietary vitamin A.

    prom1 mutants exhibit fused rhabdomeres, with a marked loss of inter-rhabdomeral space.

    eycprom-1 mutant adults have eyes with apparently normal microvilli. However, these eyes show a striking failure of the rhabdomeres to separate from each other, including a marked loss of inter-rhabdomeral space, irregular rhabdomere morphology and improper rhabdomeral contacts.

    prom1 mutant adults have eyes with apparently normal microvilli. However, these eyes show a striking failure of the rhabdomeres to separate from each other, including a marked loss of inter-rhabdomeral space, irregular rhabdomere morphology and improper rhabdomeral contacts.

    In the eyes of eycprom-1 flies, almost all of the rhabdomeres fail to separate from one another, so that in most ommatidia only one cluster of rhabdomeres can be observed instead of 7 individual rhabdomeres.

    In the eyes of prom1 flies, almost all of the rhabdomeres fail to separate from one another, so that in most ommatidia only one cluster of rhabdomeres can be observed instead of 7 individual rhabdomeres.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by Rho1JF02809/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by RokJF03225/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by MbsN300.UAS/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by +/Df(1)ED6829

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by Act5CG0245/Act5C[+]

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by zipHMS01618/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by zipDN.UAS.GFP/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by sqhHMS00437/Scer\GAL4GMR.PU

    eys1, prom[+]/prom1 has retina phenotype, enhanceable by snaHMS01252/Scer\GAL4GMR.PU

    chp[+]/chp2, prom1 has rhabdomere phenotype, enhanceable by eys[+]/eys1

    chp[+]/chp2, prom1 has ommatidium phenotype, enhanceable by eys[+]/eys1

    chp2, eyc[+]/prom1 has rhabdomere phenotype, enhanceable by eys[+]/eys1

    chp2, eyc[+]/prom1 has ommatidium phenotype, enhanceable by eys[+]/eys1

    Suppressed by
    NOT suppressed by
    Statement
    Reference
    Other
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The prom1 ommatidia phenotype is largely suppressed by crb11A22. Most of the rhabdomeres are individualised and a single, continuous inter-rhabdomeral space is formed.

    Flies trans-heterozygous for prom1 and eys1 show partial fusion of rhabdomeres. The phenotype is variable along the length of the retina.

    The prom1/+, eys1/+ fusion phenotype is suppressed along the entire length of the rhabdomeres by heterozygous crb11A22.

    One copy of both eys1 and prom1 results in a partial failure to form a continuous open inter-rhabdomeral space (retinal lumen), exhibited by the presence of juxtaposed rhabdomeres. This phenotype is significantly enhanced by one copy of either Df(1)ED6829 or Act5CG0245.

    Expression of zipHMS01618 under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of zipDN.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of sqhHMS00437 under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of sqhE20E21.Scer\UAS.cCa under the control of Scer\GAL4GMR.PU and Scer\GAL4Pph13.1.6 suppresses the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of snaHMS01252 under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of Rho1JF02809 under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of RokJF03225 under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Expression of MbsN300.Scer\UAS under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

    Although the rhabdomere phenotype is recessive in eys1 and eycprom-1 single mutants, eys1/+, eycprom-1/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.

    eycprom-1/eycprom-1; chp2/+ double mutant flies show suppression of the eycprom-1/eycprom-1 rhabdomeral phenotype. However, in eycprom-1/eycprom-1, eys1/+; chp2/+ flies the rhabdomeres are stuck together, so the mild eycprom-1/eycprom-1; chp2/+ phenotype is enhanced in eycprom-1/eycprom-1, eys1/+; chp2/+ flies.

    Although the rhabdomere phenotype is recessive in eys1 and prom1 single mutants, eys1/+, prom1/+ double mutant eyes show a failure of rhabdomere separation and loss of a continuous inter-rhabdomeral space.

    prom1/prom1; chp2/+ double mutant flies show suppression of the prom1/prom1 rhabdomeral phenotype. However, in prom1/prom1, eys1/+; chp2/+ flies the rhabdomeres are stuck together, so the mild prom1/prom1; chp2/+ phenotype is enhanced in prom1/prom1, eys1/+; chp2/+ flies.

    In eycprom-1/eycprom-1; chp2/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in eycprom-1/eycprom-1 single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In eycprom-1/eycprom-1, eys1/+; chp2/+ triple mutant flies, the rescue of the eycprom-1/eycprom-1 phenotype is reduced compared to the eycprom-1/eycprom-1; chp2/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

    In prom1/prom1; chp2/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in prom1/prom1 single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In prom1/prom1, eys1/+; chp2/+ triple mutant flies, the rescue of the prom1/prom1 phenotype is reduced compared to the prom1/prom1; chp2/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

    Xenogenetic Interactions
    Statement
    Reference

    Expression of Hsap\EYSScer\UAS.cNa and Hsap\PROM1Scer\UAS.cNa in eys1, prom1 double mutants results in rescue of the eye phenotype, with separation of the rhabdomeres and the presence of inter-rhabdomeral space.

    Expression of Hsap\PROM1Scer\UAS.cNa in photoreceptor cells under the control of Scer\GAL4Pph13 or Scer\GAL4GMR.PF suppresses the prom1 mutant phenotype.

    Expression of Hsap\PROM1mut1-7.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF fails to suppress the prom1 mutant eye phenotype.

    Expression of Hsap\PROM1ΔC.Scer\UAS.T:Avic\GFP in the developing eye under the control of Scer\GAL4GMR.PF suppresses the prom1 mutant eye phenotype.

    Expression of Hsap\PROM1R373C.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF, is not sufficient to suppress the rhabdomere phenotype in prom1 mutants.

    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    FlyBase curator comment: The prom1 allele was previously referred to as "eycprom-1" in FlyBase due to confusion over which transcription unit corresponds to the "eyc" complementation group. However, FBrf0198649 shows that the lesion in the mutant maps within the prom transcription unit, and thus the allele is now listed as an allele of prom in FlyBase.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (9)