Allele Dmel\unc-5⁸

General Information
Symbol	Dmel\unc-5⁸	Species	D. melanogaster
Name		FlyBase ID	FBal0193802
Feature type	allele	Associated gene	Dmel\unc-5

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Labrador, 2005)
Mutagen	ethyl methanesulfonate (Labrador, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q19@. (Labrador, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective (Labrador, 2005) neuroanatomy defective (with Df(2R)XTE-18) (Labrador, 2005) neuroanatomy defective (with unc-5²) (Labrador, 2005) neuroanatomy defective \| embryonic stage (von Hilchen et al., 2010)
Phenotype Manifest In
	abdominal segmental nerve (Labrador, 2005) abdominal segmental nerve (with Df(2R)XTE-18) (Labrador, 2005) abdominal segmental nerve (with unc-5²) (Labrador, 2005) embryonic peripheral glial cell 5 \| embryonic stage (von Hilchen et al., 2010) embryonic peripheral glial cell 6 \| embryonic stage (von Hilchen et al., 2010) embryonic peripheral glial cell 8 \| embryonic stage (von Hilchen et al., 2010) peripheral glial cell \| embryonic stage (von Hilchen et al., 2010)
Detailed Description
	Statement Reference The number of embryonic peripheral glia (ePG) present on the intersegmental nerve distal to the SNc nerve branch is reduced compared to wild type. Positional and marker expression analysis indicates that it is the ePG6 and ePG8 cells which are missing from the intersegmental nerve distal to the SNc nerve branch, and they are instead either stalled in the exit area proximal to the SNc or fail to leave the central nervous system. ePG5 also occasionally stalls in the exit area. (von Hilchen et al., 2010) 28% of unc-5⁸ mutants show a SNa guidance phenotype; 22% of unc-5⁸/Df(2R)XTE-18 and 19% of unc-5⁸/unc-5² transheterozygotes also show this phenotype. (Labrador, 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Df(2R)eve, eve^ΔRP2A, unc-5⁸ has neuroanatomy defective phenotype (Zarin et al., 2012) grn^7L, unc-5[+]/unc-5⁸ has neuroanatomy defective \| embryonic stage phenotype (Zarin et al., 2012)
Phenotype Manifest In
Other
Statement Reference Df(2R)eve, eve^ΔRP2A, unc-5⁸ has intersegmental nerve phenotype (Zarin et al., 2012) grn^7L, unc-5[+]/unc-5⁸ has intersegmental nerve phenotype (Zarin et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference eve[ΔRP2A], Df(2R)eve/+ ; unc-5[8]/+ double heterozygous embryos show guidance defects in the intersegmental nerve. unc-5[8]/+ ; grn[7L]/+ double heterozygous embryos show guidance defects in the intersegmental nerve. (Zarin et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	unc-5⁸/unc-5² is partially rescued by unc-5^{Scer\UAS.T:Ivir\HA1,T:SS-wg}/Scer\GAL4^elav.PLu (Labrador, 2005) unc-5⁸ is partially rescued by unc-5^{Scer\UAS.T:Ivir\HA1,T:SS-wg}/Scer\GAL4^cas-3921 (von Hilchen et al., 2010) unc-5⁸ is partially rescued by unc-5^{Scer\UAS.T:Ivir\HA1,T:SS-wg}/Scer\GAL4^repo (von Hilchen et al., 2010)
Comments	Expression of unc-5[Scer\UAS.T:Ivir\HA1,T:SS-wg] under the control of Scer\GAL4[cas-3921] in unc-5[8] embryos rescues the normal number of six embryonic peripheral glia distal to the SNc in 49% of hemisegments (compared to only 6% of hemisegments in unc-5[8] embryos) and five ePG are found distal to the SNc in 39% of hemisegments (compared to only 24% of hemisegments in unc-5[8] embryos). Expression of unc-5[Scer\UAS.T:Ivir\HA1,T:SS-wg] under the control of Scer\GAL4[repo] in unc-5[8] embryos rescues the normal number of six embryonic peripheral glia distal to the SNc in 49% of hemisegments (compared to only 6% of hemisegments in unc-5[8] embryos) and five ePG are found distal to the SNc in 33% of hemisegments (compared to only 24% of hemisegments in unc-5[8] embryos). (von Hilchen et al., 2010) Expression of unc-5^{Scer\UAS.T:Ivir\HA1,T:wg}, under the control of Scer\GAL4^elav.PLu, partially rescues the ISN and SNa defects of unc-5²/unc-5⁸ mutants. (Labrador, 2005)
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: a suppressor of the unc-5^GS; Scer\GAL4^scrt-GAL4 lethal phenotype. (Labrador, 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	unc-5⁸ (Zarin et al., 2012) Unc-5⁸ (Labrador, 2005) unc5⁸ (von Hilchen et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Zarin et al., 2012, Development 139(10): 1798--1805 A GATA/homeodomain transcriptional code regulates axon guidance through the Unc-5 receptor. [FBrf0218039] von Hilchen et al., 2010, Development 137(8): 1251--1262 Netrins guide migration of distinct glial cells in the Drosophila embryo. [FBrf0210391]
Supplementary material	Labrador, 2005, Curr. Biol. 15(15): [FBrf0198672]

Allele Dmel\unc-58

Allele Dmel\unc-5⁸