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General Information
Symbol
Dmel\mir-7UAS.cLb
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct b of Li
FlyBase ID
FBal0193850
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-miR-7
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 432bp fragment containing the mir-7 precursor. The mir-7 fragment contains no HnRNP-K coding sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing mir-7Scer\UAS.cLb under the control of Scer\GAL4ey.PH does not cause any overt eye overgrowth.

The number of spermatogonial cysts per testis is increased in males expressing mir-7Scer\UAS.cLb under the control of Scer\GAL4nos.PG compared to control males.

Expression of mir-7Scer\UAS.cLb under the control of Scer\GAL4GMR.PF leads to rough eyes. Expression in the larval eye disc causes the production of ectopic R7 photoreceptor neurons in ommatidia, which can still be observed at later stages of eye development.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of mir-7Scer\UAS.cLb and DlScer\UAS.cDa under the control of Scer\GAL4ey.PH results in overgrown eye discs, resulting in overgrown and folded eyes in 70% of flies.

Expression of boiScer\UAS.cHa fully suppresses the eye disc overgrowth seen when mir-7Scer\UAS.cLb and DlScer\UAS.cDa are co-expressed under the control of Scer\GAL4ey.PH.

Expression of hhScer\UAS.cCa suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. The phenotype is seen with 100% penetrance.

Expression of ciScer\UAS.T:Ivir\HA1 partially suppresses the eye overgrowth phenotype seen when DlScer\UAS.cDa and mir-7Scer\UAS.cLb are expressed under the control of Scer\GAL4ey.PH. Many eyes are substantially reduced in size but still exhibit abnormal patterned growth.

The increase in the number of spermatogonial cysts per testis that is seen in males expressing mir-7Scer\UAS.cLb under the control of Scer\GAL4nos.PG is suppressed if they are also expressing bamhs.PO.

Coexpression of the EgfrDN.Scer\UAS and mir-7Scer\UAS.cLb transgenes, under the control of Scer\GAL4GMR.PF, results in ommatidia with ectopic photoreceptor cells. The positions of these cells suggest they are R3 and R4.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
mir-7Scer\UAS.cLb
mir-7UAS.cLb
Name Synonyms
Saccharomyces cerevisiae UAS construct b of Li
Secondary FlyBase IDs
    References (4)