FlyBase Allele Report: Dmel\S6kII[UAS.Tag:MYC]

General Information

Symbol

Dmel\S6kII^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0194033

Feature type

allele

Associated gene

Dmel\S6kII

Associated Insertion(s)

Carried in Construct

P{UAS-RSK.WT.MYC}

Key Links

Transgenic product class

wild_type

(Kim et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kim et al., 2006)

Mutagen

in vitro construct

(Kim et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-RSK.WT.MYC}

Cytology

Description

UASt regulatory sequences drive expression of wild-type S6kII, tagged with Tag:MYC.

(Kim et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

S6kII

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Kim et al., 2006)

Phenotype Manifest In

interommatidial bristle, with Scer\GAL4^GMR.PF

(Kim et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Kim et al., 2006)

rhabdomere, with Scer\GAL4^GMR.PF

(Kim et al., 2006)

wing vein, with Scer\GAL4^Bx-MS1096

(Kim et al., 2006)

wing vein, with Scer\GAL4^en-e16E

(Kim et al., 2006)

Detailed Description

Statement

Reference

Expression of S6kII^{Scer\UAS.T:Hsap\MYC} in the eye, driven by Scer\GAL4^GMR.PF, induces a rough eye phenotype with a dramatically reduced number of rhabdomeres. At the pupal level, eyes show a reduction in differentiation of cells into both neuronal and non-neuronal retinal cells.

Expression of S6kII^{Scer\UAS.T:Hsap\MYC} under the control of either Scer\GAL4^en-e16E or Scer\GAL4^Bx-MS1096 induces compartment-specific vein loss in the wing.

(Kim et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Ras85D^e1B/Ras85D[+]

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by phl[+]/Raf¹²

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by rl[+]/rl^10a

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Dsor1[+]/Dsor1^LH110

(Kim et al., 2006)

Suppressor of

Statement

Reference

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by rl[+]/rl^10a

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by rl[+]/rl^10a

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has rhabdomere phenotype, enhanceable by Ras85D^e1B/Ras85D[+]

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by phl[+]/Raf¹²

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by phl[+]/Raf¹²

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has rhabdomere phenotype, enhanceable by phl[+]/Raf¹²

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has rhabdomere phenotype, enhanceable by rl[+]/rl^10a

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Dsor1[+]/Dsor1^LH110

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by Dsor1[+]/Dsor1^LH110

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has rhabdomere phenotype, enhanceable by Dsor1[+]/Dsor1^LH110

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Ras85D^e1B/Ras85D[+]

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by Ras85D^e1B/Ras85D[+]

(Kim et al., 2006)

Suppressor of

Statement

Reference

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of interommatidial bristle phenotype of Ras85D^V12.UAS, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of ommatidium phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of interommatidial bristle phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a suppressor of rhabdomere phenotype of Raf^UAS.F179, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Scer\GAL4^hs.2sev/S6kII^UAS.Tag:MYC is a suppressor of rhabdomere phenotype of Ras85D^V12.UAS

(Kim et al., 2006)

Scer\GAL4^hs.2sev/S6kII^UAS.Tag:MYC is a suppressor of eye photoreceptor cell | increased number phenotype of sev^S11.Tag:MYC

(Kim et al., 2006)

NOT Suppressor of

Statement

Reference

S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev is a non-suppressor of eye photoreceptor cell | increased number phenotype of Scer\GAL4^hs.2sev, rl^{Sem.UAS.Tag:HA}

(Kim et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of S6kII^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a rl^10a/+, Dsor1^LH110/+, Ras85D^e1B/+ or phl¹²/+ background than when expressed in a wild-type background.

The roughened eye and increased rhabdomere phenotypes seen when either phl^{Scer\UAS.F179} or Ras85D^V12.Scer\UAS is expressed under the control of Scer\GAL4^hs.2sev are suppressed when S6kII^{Scer\UAS.T:Hsap\MYC} is coexpressed. Scer\GAL4^hs.2sev>S6kII^{Scer\UAS.T:Hsap\MYC} expression also suppresses the increased photoreceptor cell phenotype seen in sev^{S11.T:Hsap\MYC} flies.

Coexpression of S6kII^{Scer\UAS.T:Hsap\MYC} fails to suppress the increased photoreceptor cell phenotype of Scer\GAL4^hs.2sev>rl^{Sem.Scer\UAS.T:Ivir\HA1} flies.

(Kim et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/S6kII^UAS.Tag:MYC rescues S6kII^D1

(Kim et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

RSK^WT

(Kim et al., 2006)

S6kII^{Scer\UAS.T:Hsap\MYC}

S6kII^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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