FlyBase Allele Report: Dmel\rl[Sem.UAS.Tag:HA]

General Information

Symbol

Dmel\rl^{Sem.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0194040

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{UAS-ERK.Sem}

Key Links

Transgenic product class

missense_variant

(Kim et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Kim et al., 2006)

Mutagen

in vitro construct

(Kim et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-ERK.Sem}

Cytology

Description

Amino acid replacement: D334N.

(Kim et al., 2006)

UAS regulatory sequences drive expression of a constitutive active form of rl, tagged with Tag:HA.

(Kim et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

visible | adult stage, with Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

Phenotype Manifest In

eye photoreceptor cell | increased number, with Scer\GAL4^hs.2sev

(Kim et al., 2006)

wing vein | ectopic, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

wing vein | ectopic, with Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

Detailed Description

Statement

Reference

Expression of rl^{Sem.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^MD-638 results in moderate ectopic wing vein phenotypes. A weak ectopic vein phenotype is seen when rl^{Sem.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

(Molnar and de Celis, 2013)

The eyes of flies that express rl^{Sem.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev show a greater number of photoreceptor cells per ommatidium than wild-type flies.

(Kim et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, rl^{Sem.UAS.Tag:HA} has visible | adult stage phenotype, enhanceable by tay^RNAi.UAS/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has visible | adult stage phenotype, enhanceable by Hsap\AUTS2^UAS.Tag:FLAG, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

NOT suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by tay^{FL.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has visible | adult stage phenotype

(Molnar and de Celis, 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, rl^{Sem.UAS.Tag:HA} has wing vein | ectopic phenotype, enhanceable by tay^RNAi.UAS/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has wing vein | ectopic phenotype, enhanceable by Hsap\AUTS2^UAS.Tag:FLAG, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

NOT suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has wing vein | ectopic phenotype, non-suppressible by tay^{FL.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

Scer\GAL4^hs.2sev, rl^{Sem.UAS.Tag:HA} has eye photoreceptor cell | increased number phenotype, non-suppressible by S6kII^UAS.Tag:MYC, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^salm.EPv, rl^{Sem.UAS.Tag:HA} has wing vein | ectopic phenotype

(Molnar and de Celis, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of rl^{Sem.Scer\UAS.T:Ivir\HA1} and tay^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MD-638 results in a stronger ectopic wing vein phenotype than when either transgene is expressed alone.

Co-expression of Ras85D^V12.Scer\UAS and rl^{Sem.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^salm.EPv results in a strong ectopic wing vein phenotype.

Expression of tay^{FL.Scer\UAS.T:Zzzz\FLAG} does not suppress the ectopic wing vein phenotype seen when Ras85D^V12.Scer\UAS and rl^{Sem.Scer\UAS.T:Ivir\HA1} are co-expressed under the control of Scer\GAL4^salm.EPv.

(Molnar and de Celis, 2013)

Coexpression of S6kII^{Scer\UAS.T:Hsap\MYC} fails to suppress the increased photoreceptor cell phenotype of Scer\GAL4^hs.2sev>rl^{Sem.Scer\UAS.T:Ivir\HA1} flies.

(Kim et al., 2006)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\AUTS2^{Scer\UAS.T:Zzzz\FLAG} enhances the ectopic wing vein phenotype seen when rl^{Sem.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^salm.EPv.

(Molnar and de Celis, 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rl^{Sem.Scer\UAS.T:Ivir\HA1}

rl^{Sem.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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