|Feature type||allele||Associated gene||Dmel\Tequila|
|Mutagen||in vitro construct - regulatory fusion, in vitro construct - RNAi|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
1 day memory is impaired after spaced training, but not after massed training in an olfactory associative conditioning assay in flies expressing Tequila[dsRNA.Scer\UAS] under the control of Scer\GAL4[G0431]. The defect in memory after spaced training is still seen if the temperature sensitive Scer\GAL80[ts.αTub84B] allele is used to restrict Scer\GAL4 expression to the adult stage.
Expression of Tequila[dsRNA.Scer\UAS] in the adult mushroom bodies, under the control of Scer\GAL4[Mef2.247.Switch] induces defects in both sugar response and short-term memory.
RNAi-mediated Tequila knockdown, achieved by expression of TequiladsRNA.Scer\UAS under the control of Scer\GAL4Mef2.247, results in a decrease in 24-hour long-term memory. 24-hour memory after massed training is unaffected , as are learning and 2-hour memory after single conditioning. No alteration of mushroom body morphology can be observed. The long-term memory phenotype induced by RNAi knockdown is reversible as flies expressing TequiladsRNA.Scer\UAS driven by Scer\GAL4Mef2.247.Switch show the phenotype while flies are fed with RU486, but show a normal 24-hour long term memory capacity following transferral to food without RU846, thus preventing Scer\GAL4Mef2.247.Switch from driving TequiladsRNA.Scer\UAS expression.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 4 )|