Allele Dmel\DAAM^Ex1

General Information
Symbol	Dmel\DAAM^Ex1	Species	D. melanogaster
Name		FlyBase ID	FBal0194068
Feature type	allele	Associated gene	Dmel\DAAM

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Matusek et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}EP1542 (Matusek et al., 2006)
Nature of the lesion	Statement Reference Imprecise excision of the P{EP}EP1542 element, resulting in deletion of most of the 3'UTR and a very small part of the C-terminal end of the coding region. (Matusek et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Matusek et al., 2006) lethal (with DAAM^Ex36) (Matusek et al., 2006) lethal (with DAAM^Ex68) (Matusek et al., 2006) neuroanatomy defective (with DAAM^Ex68) (Matusek et al., 2008) partially lethal - majority die (Matusek et al., 2006) viable (with DAAM^Ex65) (Matusek et al., 2006) viable (with DAAM^Ex184) (Matusek et al., 2006) viable (with DAAM^Ex202) (Matusek et al., 2006) viable (with DAAM^Ex249) (Matusek et al., 2006)
Phenotype Manifest In
	connective (with DAAM^Ex68) (Matusek et al., 2008) embryo (with DAAM^Ex68) (Matusek et al., 2008) embryonic/larval trachea (Matusek et al., 2006) fascicle (with DAAM^Ex68) (Matusek et al., 2008) filopodium (with DAAM^Ex68) (Gonçalves-Pimentel et al., 2011) lateral longitudinal fascicle (with DAAM^Ex68) (Matusek et al., 2008) neuropil (with DAAM^Ex68) (Matusek et al., 2008)
Detailed Description
	Statement Reference Cultured primary neurons derived from DAAM[Ex1]/DAAM[Ex68] embryos show a significant reduction in the number of filopodia compared to control neurons. In live analyses, the mutant neurons show modestly increased protrusion rates and strongly increased retraction rates of filopodia. (Gonçalves-Pimentel et al., 2011) DAAM^Ex1 flies show 17% viability compared to wild-type flies. DAAM^Ex1/DAAM^Ex249 adults show no defects in the eye or wing. Trachea dissected out from third instar DAAM^Ex1 mutant larvae display a moderate tracheal cuticle phenotype. (Matusek et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference DAAM^Ex1 has partially lethal - majority die phenotype, enhanceable by Btk29A^k05610 (Matusek et al., 2006) DAAM^Ex1 has partially lethal - majority die phenotype, enhanceable by Rho1^72F (Matusek et al., 2006) DAAM^Ex1 has partially lethal - majority die phenotype, enhanceable by Src42A^E1 (Matusek et al., 2006)
Phenotype Manifest In
Enhanced by
Statement Reference DAAM^Ex1/DAAM^Ex68 has filopodium phenotype, enhanceable by Arpc1¹ (Gonçalves-Pimentel et al., 2011) DAAM^Ex1 has embryonic/larval trachea phenotype, enhanceable by Btk29A^k05610 (Matusek et al., 2006) DAAM^Ex1 has embryonic/larval trachea phenotype, enhanceable by Rho1^72F (Matusek et al., 2006) DAAM^Ex1 has embryonic/larval trachea phenotype, enhanceable by Src42A^E1 (Matusek et al., 2006)
Enhancer of
Statement Reference DAAM^Ex1/DAAM^Ex68 is an enhancer of filopodium phenotype of Arpc1¹ (Gonçalves-Pimentel et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Cultured primary neurons derived from DAAM[Ex1]/DAAM[Ex68] Sop2[1] embryos show a very strong reduction in the number of filopodia (to 5% of the wild type average) compared to control neurons. Only 20% of cultured primary neurons derived from DAAM[Ex1]/DAAM[Ex68] Sop2[1]/Sop2[Q25sd] embryos have neurites. (Gonçalves-Pimentel et al., 2011) The tracheal cuticle phenotype of DAAM^Ex1 larvae is enhanced by Rho1^72F, Src42A^E1 and Btk29A^k05610. These alleles also decrease the viability of the semilethal DAAM^Ex1 larvae. (Matusek et al., 2006)
Xenogenetic Interactions
Statement Reference Expression of Mmus\Daam1[Scer\UAS.T:Avic\GFP-EGFP] pan-neuronally under the control of Scer\GAL4[elav-C155] results axon defects in 70% of DAAM[Ex68]/DAAM[Ex1] progeny (where both maternal and zygotic DAAM is removed). (Matusek et al., 2008)
Complementation & Rescue Data
Complements	DAAM^Ex65 (Matusek et al., 2006) DAAM^Ex184 (Matusek et al., 2006) DAAM^Ex202 (Matusek et al., 2006) DAAM^Ex249 (Matusek et al., 2006)
Fails to complement	DAAM^Ex36 (Matusek et al., 2006) DAAM^Ex68 (Matusek et al., 2006)
Rescued by	DAAM^Ex1/DAAM^Ex68 is rescued by DAAM^{Scer\UAS.P\T.cMa}/Scer\GAL4^Act5C.PI (Matusek et al., 2008) DAAM^Ex1/DAAM^Ex68 is rescued by Scer\GAL4^elav-C155/DAAM^{Scer\UAS.P\T.cMa} (Matusek et al., 2008) DAAM^Ex1 is rescued by DAAM^{Scer\UAS.P\T.cMa}/Scer\GAL4^Act.PU (Matusek et al., 2006) DAAM^Ex1 is rescued by DAAM^{Scer\UAS.P\T.cMa}/Scer\GAL4^tub.PU (Matusek et al., 2006)
Comments	The CNS defects revealed in DAAM[Ex68]/DAAM[Ex1] mutants are fully rescued when DAAM[Scer\UAS.P\T.cMa] is expressed under the control of the pan-neuronal Scer\GAL4[elav-C155] or the ubiquitous Scer\GAL4[Act5C.PI] driver. (Matusek et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	DAAM^Ex1 (Matusek et al., 2006, Matusek et al., 2008, Gonçalves-Pimentel et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Gonçalves-Pimentel et al., 2011, PLoS ONE 6(3): e18340 Dissecting regulatory networks of filopodia formation in a Drosophila growth cone model. [FBrf0213367] Matusek et al., 2008, J. Neurosci. 28(49): 13310--13319 Formin proteins of the DAAM subfamily play a role during axon growth. [FBrf0206320] Matusek et al., 2006, Development 133(5): 957--966 The Drosophila formin DAAM regulates the tracheal cuticle pattern through organizing the actin cytoskeleton. [FBrf0190308]

Allele Dmel\DAAMEx1

Allele Dmel\DAAM^Ex1