The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing Toll-6C1020Y.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav.PU can be rescued by combination with wekEX14/Df(2R)BSC279. In contrast, the increase in Dcp-1-positive cells in pupal VNC induced by Scer\GAL4elav.PU-driven expression of Ect4EP3610 cannot be suppressed by loss of combination with wekEX14/Df(2L)BSC690).
The variable dorsalized embryonic phenotype of wekEX14 mutants is enhanced by mutations in the genes Tl, pll and tub. While 51% of wekEX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 97% in wekEX14; Tlr4/+ embryos, 92% in wekEX14; pll7/+ embryos and 96% in wekEX14; tub2/+ embryos.
Embryos produced by weklor/wekEX14; Tl3/+ mothers show a biphasic phenotype distribution. 76% show a moderately dorsalized phenotype; these embryos lack ventral and lateral structures but retain the dorsolaterally-derived filzkorper. 24% show a lateralized phenotype; these embryos are elongated and thin in appearance and contain fine rings of laterally-derived ventral denticle belts.
The reduced level of cell death (decreased number of Dcp-1-positive cells) observed in the ventral nerve cord (VNC) of wekEX14/Df(2L)BSC690 and the increase in cell death induced by Scer\GAL4elav.PU-driven expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 can both be mitigated by combining the two manipulations together: The number of apoptotic cells in the VNC of wekEX14/Df(2L)BSC690 mutants expressing wekScer\UAS.ORF.GW.T:Ivir\HA1 is intermediate to each of the two perturbations and higher than in controls.