FB2025_01 , released February 20, 2025
Allele: Dmel\BicDUASp.Tag:FLAG.Tag:polyHis
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General Information
Symbol
Dmel\BicDUASp.Tag:FLAG.Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0194143
Feature type
allele
Associated gene
Dmel\BicD
Associated Insertion(s)
Carried in Construct
P{UASp-BicD.FLAG.His6}
Also Known As
UAS-BicD
Key Links
Transgenic product class
wild_type
(Bullock et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bullock et al., 2006)
Mutagen
in vitro construct
(Bullock et al., 2006)
Progenitor genotype
Carried in construct
P{UASp-BicD.FLAG.His6}
Cytology
Description

UASp regulatory sequences drive expression of BicD. The protein is tagged with both Tag:FLAG and Tag:polyHis at the 3' end.

(Bullock et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
BicD
Tagged with
Tag:FLAG
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 results in no obvious dendrite morphology defects in third instar larval class I ddaD and ddaE (under the control of Scer\GAL4221) or class IV ddaC (under the control of Scer\GAL4ppk.PG) abdominal dorsal multidendritic neurons, as compared to controls.

      (Hattori et al., 2013)

      Expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 prevents basal redistribution of lipid droplets in the blastoderm embryo, leading to an opaque cytoplasm.

      (Dienstbier et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL41032.hx/BicDUASp.Tag:FLAG.Tag:polyHis rescues BicDr5

      (Li et al., 2010)

      Scer\GAL4elav-C155/BicDUASp.Tag:FLAG.Tag:polyHis rescues BicDr5

      (Li et al., 2010)
      Partially rescues

      Scer\GAL4VGlut1-OK371/BicDUASp.Tag:FLAG.Tag:polyHis partially rescues BicDr5

      (Li et al., 2010)
      Fails to rescue

      Scer\GAL4how-24B/BicDUASp.Tag:FLAG.Tag:polyHis fails to rescue BicDr5

      (Li et al., 2010)
      Comments

      Ubiquitous expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 under the control of Scer\GAL41032.hx rescues the larval locomotion phenotype and lethality of BicDr5/Df(2L)TW119 mutants.

      Pan-neuronal expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 under the control of Scer\GAL4elav-C155 rescues the larval locomotion phenotype and lethality of BicDr5/Df(2L)TW119 mutants.

      Expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 in muscles under the control of Scer\GAL4how-24B fails to rescue the larval locomotion phenotype and lethality of BicDr5/Df(2L)TW119 mutants.

      Locomotion of BicDr5/Df(2L)TW119 mutants is significantly improved by driving expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 with the motor-neuronal driver Scer\GAL4VGlut-OK371. The synapse size and muscle size phenotypes of BicDr5/Df(2L)TW119 mutants are also rescued by Scer\GAL4VGlut-OK371-driven expression of BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6. However, this is not sufficient to fully restore locomotion or eclosion.

      The dye-release defect in BicDr5/Df(2L)TW119 mutants is fully rescued by expressing BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 using the Scer\GAL4VGlut-OK371 driver.

      (Li et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      BicDScer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6
      BicDUASp.Tag:FLAG.Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (5)