FlyBase Allele Report: Dmel\Dscam1[exon17.2.UAS.GFP]

General Information

Symbol

Dmel\Dscam1^{exon17.2.UAS.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0194155

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

Carried in Construct

P{UAS-Dscam1.exon17.2.GFP}

Also Known As

UAS-Dscam17.2-GFP

Key Links

Transgenic product class

wild_type

(Wang et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wang et al., 2004)

Mutagen

in vitro construct

(Wang et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Dscam1.exon17.2.GFP}

Cytology

Description

UAS regulatory sequences drive expression of Dscam1 which is tagged at the C-terminal end with GFP. The Dscam1 sequences contain the 17.2 alternative exon.

(Wang et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dscam1

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Okumura et al., 2015, Sakuma et al., 2015)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Okumura et al., 2015, Sakuma et al., 2015)

calyx of adult mushroom body, with Scer\GAL4^ey-OK107

(Wang et al., 2004)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.PG

(Kim et al., 2013)

larval multidendritic class III neuron | third instar larval stage, with Scer\GAL4^109(2)80

(Sterne et al., 2015)

larval multidendritic class I neuron | third instar larval stage, with Scer\GAL4^109(2)80

(Sterne et al., 2015)

larval multidendritic class IV neuron | somatic clone | third instar larval stage, with Scer\GAL4^ppk.PG

(Sterne et al., 2015)

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PG

(Sterne et al., 2015)

lobe system of adult mushroom body, with Scer\GAL4^ey-OK107

(Wang et al., 2004)

mushroom body dorsal paired medial cell, with Scer\GAL4^c316

(Keene et al., 2006)

synapse, with Scer\GAL4^ppk.PG

(Kim et al., 2013)

Detailed Description

Statement

Reference

Expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} in all mushroom body neurons under the control of Scer\GAL4^ey-OK107 causes a loss of the α and β lobes.

(Okumura et al., 2015)

Expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107 results in the loss of alpha and beta lobes of the mushroom body.

(Sakuma et al., 2015)

Ectopic expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ppk.PG in MARCM somatic clones causes presynaptic terminal overgrowth and significantly increases the presynaptic terminal length in class IV dendritic arborizing neurons in third instar larvae compared to controls.

Ectopic expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ppk.PG causes presynaptic terminal overgrowth in class IV dendritic arborizing neurons in third instar larvae compared to controls.

Ectopic expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^109(2)80 causes ectopic repulsion between class I and class III dendritic arborizing neurons in third instar larvae compared to controls.

(Sterne et al., 2015)

Overexpression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} in da neurons, under the control of Scer\GAL4^ppk.PG generates abnormally long presynaptic arbors, resulting in a 2.7-fold increase in presynaptic terminal length.

(Kim et al., 2013)

Expression of Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} in dorsal paired medial neurons, driven by Scer\GAL4^c316, affects the development of these neurons and results in their predominant projection into the mushroom body α and bgr; lobe subsets. Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} expression does not significantly affect punitive-odor and reward-odor memory.

(Keene et al., 2006)

Animals expressing Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^OK107 show drastic defects in mushroom body axon projections. Mushroom body lobes are missing or misshapen and the mushroom body calyx is reduced in size. The peduncle is normal-looking.

(Wang et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^c316 has abnormal memory | adult stage phenotype, non-enhanceable by Dscam1^{exon17.2.UAS.GFP}

(Krashes et al., 2007)

Suppressed by

Statement

Reference

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has abnormal neuroanatomy phenotype, suppressible by Tbcd^RNAi.UAS.cOa, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has abnormal neuroanatomy phenotype, suppressible by TBCD[+]/Tbcd¹

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Strip[+]/Strip^dogi

(Sakuma et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ppk.PG has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Abl¹/Abl¹

(Sterne et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ppk.PG has abnormal neuroanatomy | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Abl⁴/Abl⁴

(Sterne et al., 2015)

NOT Enhancer of

Statement

Reference

Dscam1^{exon17.2.UAS.GFP} is a non-enhancer of abnormal memory | adult stage phenotype of Scer\GAL4^c316, shi^1.UAS

(Krashes et al., 2007)

Dscam1^{exon17.2.UAS.GFP} is a non-enhancer of abnormal memory | adult stage phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^c316

(Krashes et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body alpha-lobe phenotype, suppressible by Tbcd^RNAi.UAS.cOa, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body alpha-lobe phenotype, suppressible by TBCD[+]/Tbcd¹

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body beta-lobe phenotype, suppressible by Tbcd^RNAi.UAS.cOa, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body beta-lobe phenotype, suppressible by TBCD[+]/Tbcd¹

(Okumura et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body alpha-lobe phenotype, suppressible by Strip[+]/Strip^dogi

(Sakuma et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107 has adult mushroom body beta-lobe phenotype, suppressible by Strip[+]/Strip^dogi

(Sakuma et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ppk.PG has larval multidendritic class IV neuron | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Abl¹/Abl¹

(Sterne et al., 2015)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ppk.PG has larval multidendritic class IV neuron | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by Abl⁴/Abl⁴

(Sterne et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of TBCD^{miRNA.Scer\UAS} partially suppresses the loss of mushroom body α and β lobes seen in flies expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107.

A TBCD¹/+ mutant background partially suppresses the loss of mushroom body α and β lobes seen in flies expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107.

(Okumura et al., 2015)

The loss of alpha and beta lobes of the mushroom body observed in brains of adult flies expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107 is partially restored by combination with Strip^dogi in heterozygous state.

(Sakuma et al., 2015)

The increased presynaptic terminal length observed in class IV dendritic arborizing neurons in somatic MARCM clones expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ppk.PG in third instar larvae is completely abolished in clones that are also homozygous mutant for either Abl¹ or Abl⁴.

The ectopic repulsion between between class I and class III dendritic arborizing neurons in third instar larvae observed upon expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^109(2)80 cannot be rescued by combination with Abl¹/Abl⁴.

(Sterne et al., 2015)

Expression of shi^1.Scer\UAS with Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} by Scer\GAL4^c316, and transferring the animals to 31[o]C for one hour immediately after training significantly reduces 3hr aversive odour memory compared to controls.

(Krashes et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR27G12, Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^GMR37G09 rescues Dscam1²¹

(Bui and Kamiyama, 2024)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^GMR23E04, Scer\GAL4^eve.RN2 rescues Dscam1²¹/Dscam1²³

(Kamiyama et al., 2015)

Partially rescues

Dscam1^{exon17.2.UAS.GFP}/Scer\GAL4^ey-OK107 partially rescues Dscam1^RNAi.UAS.18

(Shi et al., 2007)

Fails to rescue

Scer\GAL4^GMR27G12/Dscam1^{exon17.2.UAS.GFP} fails to rescue Dscam1²¹

(Bui and Kamiyama, 2024)

Dscam1^{exon17.2.UAS.GFP}/Scer\GAL4^GMR37G09 fails to rescue Dscam1²¹

(Bui and Kamiyama, 2024)

Dscam1^{exon17.2.UAS.GFP}/Scer\GAL4^eve.RN2 fails to rescue Dscam1²¹/Dscam1²³

(Kamiyama et al., 2015)

Comments

The dendritogenesis defects (reduced number of dendritic tips) in the embryonic aCC motoneurons characteristic for Dscam1²³/Dscam1²¹ transheterozygotes cannot be rescued by expression of Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^eve.RN2 but is fully rescued when both Scer\GAL4^eve.RN2 and Scer\GAL4^GMR23E04 driver are used simultaneously - but only in the aCC that are associated with MP1 neurons expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} (Scer\GAL4^GMR23E04 drives expression in a random half of all MP1 cells).

(Kamiyama et al., 2015)

Co-expression of Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} partially rescues the mushroom body phenotype of Dscam^{miRNA.Scer\UAS.18} Scer\GAL4^ey-OK107 animals.

(Shi et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

FlyBase curator comment: Dscam^{exon17.2.Scer\UAS.T:Avic\GFP} is used to record information in FlyBase when authors use one of the following alternative exon 17.2 containing UAS-Dscam-GFP constructs described in FBrf0180573, but do not specify which one was used; Dscam^{A-17.2+19+23.Scer\UAS.T:Avic\GFP}, Dscam^{A-17.2+19-23.Scer\UAS.T:Avic\GFP}, Dscam^{A-17.2-19+23.Scer\UAS.T:Avic\GFP}, Dscam^{A-17.2-19-23.Scer\UAS.T:Avic\GFP}, Dscam^{B-17.2+19+23.Scer\UAS.T:Avic\GFP}, Dscam^{B-17.2+19-23.Scer\UAS.T:Avic\GFP}.

(Wang et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP}

Dscam1^{exon17.2.UAS.GFP}

Dscam^{exon17.2.Scer\UAS.T:Avic\GFP}

Name Synonyms

Secondary FlyBase IDs

References (14)

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