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General Information
Symbol
Dmel\ykiB5
Species
D. melanogaster
Name
FlyBase ID
FBal0194179
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    ykiB5 wing discs clones occupy a severely smaller disc area than control clones.
    ykiB5 heterozygotes do not show obvious defects in larval or blood-brain barrier, larval subperineurial glial cell ploidy or nucleus size, or larval brain volume, as compared to controls.
    ykiB5 homozygous mutant embryos do not display any significant increase in the frequency of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage.
    ykiB5 homozygous mutants - rescued with heat-shock induced expression of ykihs.PW every 2 days until adulthood as otherwise larval lethal - display tumorous female germaria.
    ykiB5 mutant eye disc clones grow poorly, comprising only 1.4% of the tissue. ykiB5 mutant clones are hard to recover in stage 7 egg chambers, and cannot be recovered beyond stage 7.
    ykiB5 mutant wing disc clones cannot be recovered.
    Homozygous clones in the eye disc are very small.
    Wing disc clones mutant for ykiB5 are very small in size. Adjacent wild-type clones grow significantly more than control sibling clones.
    ykiB5 mutant MARCM clones are comparable to wild-type cell clones.
    Homozygous clones are rarely recovered in the wing disc and are small.
    ykiB5 mutant eye disc clones grow poorly compared to controls.
    Homozygous clones in the eye disc are smaller than their wild-type twin spots. Homozygous clones are rarely recovered in third larval instar wing discs.
    Eye and wing imaginal discs heterozygous for ykiB5 are normal in size and morphology and are indistinguishable from wild-type discs.
    ykiB5 mutant clones in the anterior of the wing disc grow poorly and are very small.
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    NOT suppressed by
    Statement
    Reference
    Enhancer of
    Statement
    Reference
    yki[+]/ykiB5 is an enhancer of visible phenotype of sdETX4
    yki[+]/ykiB5 is an enhancer of size defective phenotype of Scer\GAL4GMR.PF, sdUAS.cSa
    yki[+]/ykiB5 is an enhancer of visible phenotype of Scer\GAL4GMR.PF, sdUAS.cSa
    yki[+]/ykiB5 is an enhancer of size defective phenotype of Scer\GAL4vg.PU, sdUAS.cSa
    yki[+]/ykiB5 is an enhancer of visible phenotype of Scer\GAL4vg.PU, sdUAS.cSa
    NOT Enhancer of
    Statement
    Reference
    yki[+]/ykiB5 is a non-enhancer of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU
    Suppressor of
    Statement
    Reference
    yki[+]/ykiB5 is a suppressor | partially of size defective | larval stage phenotype of mir-285KO.w-
    ykiB5 is a suppressor of neoplasia | somatic clone phenotype of slmbUU11
    yki[+]/ykiB5 is a suppressor of small body phenotype of Pez2/Pez1
    yki[+]/ykiB5 is a suppressor of lethal | pupal stage phenotype of kibra1/kibra02404
    yki[+]/ykiB5 is a suppressor of increased cell number | somatic clone | larval stage phenotype of ft8
    NOT Suppressor of
    Statement
    Reference
    yki[+]/ykiB5 is a non-suppressor of increased cell number | somatic clone phenotype of Apc2g10, ApcQ8
    yki[+]/ykiB5 is a non-suppressor of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU
    yki[+]/ykiB5 is a non-suppressor of visible phenotype of hidGMR.PG/WGMR.PG
    Other
    Phenotype Manifest In
    Suppressed by
    NOT suppressed by
    Enhancer of
    Statement
    Reference
    yki[+]/ykiB5 is an enhancer of wing phenotype of sdETX4
    yki[+]/ykiB5 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, sdUAS.cSa
    yki[+]/ykiB5 is an enhancer of wing phenotype of Scer\GAL4vg.PU, sdUAS.cSa
    NOT Enhancer of
    Statement
    Reference
    yki[+]/ykiB5 is a non-enhancer of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU
    Suppressor of
    Statement
    Reference
    yki[+]/ykiB5 is a suppressor | partially of perineurium | larval stage phenotype of mir-285KO.w-
    yki[+]/ykiB5 is a suppressor | partially of perineurium | adult stage phenotype of mir-285KO.w-
    yki[+]/ykiB5 is a suppressor of septate junction | larval stage phenotype of mir-285KO.w-
    yki[+]/ykiB5 is a suppressor of embryonic/larval brain | larval stage phenotype of mir-285KO.w-
    yki[+]/ykiB5 is a suppressor | partially of nucleus | larval stage phenotype of mir-285KO.w-
    ykiB5 is a suppressor of wing disc | somatic clone phenotype of slmbUU11
    yki[+]/ykiB5 is a suppressor of eye phenotype of Scer\GAL4GMR.PU, diaCA.UAS.Tag:HA
    yki[+]/ykiB5 is a suppressor of eye | somatic clone phenotype of gfr1
    yki[+]/ykiB5 is a suppressor of eye disc | third instar larval stage phenotype of ft8/ft422
    yki[+]/ykiB5 is a suppressor of eye disc | somatic clone | larval stage phenotype of ft8
    NOT Suppressor of
    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    ykiB5/+ fails to suppress the reduced growth seen in posterior midgut wild type clones when in the presence of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones, and suppresses the growth of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones.
    ykiB5 heterozygosity suppresses the tumor phenotype of scrib1 homozygous third instar larval wing discs.
    The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in cno2/+;ykiB5/+ double heterozygous embryos compared to either of the single heterozygotes or wild type. The high frequency of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage characteristic for matse03077 homozygous mutant embryos is increased further by combination with ykiB5 in homozygous state.
    The rough eye phenotype observed in adults expressing MagiScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU is not modified by combination with single copy of ykiB5.
    ykiB5 suppresses the increase in intestinal stem cell clone size seen when TaoGD8262 is expressed under the control of Scer\GAL4Act.PU. ykiB5 suppresses the increase in intestinal stem cell clone size seen when TaodsRNA.shRNA.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.
    Expression of Ncoa6::sdDB.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tub.PU partially suppresses the growth defect seen in ykiB5 mutant wing disc clones. Expression of Ncoa6Scer\UAS.cQa under the control of Scer\GAL4tub.PU does not suppress the growth defect seen in ykiB5 mutant wing disc clones.
    ykiB5 suppresses the neoplastic transformation seen in slmbUU11 mutant wing discs.
    sd12 suppresses the reduction in clone growth seen in ykiB5 mutant eye discs. The clones comprise ~15% of the eye disc. sddel suppresses the reduction in clone growth seen in ykiB5 mutant eye discs. TgiΔP partially suppresses the reduction in clone growth seen in ykiB5 mutant eye discs. The clones comprise ~7% of the eye disc.
    ykiB5/+ suppresses the wing disc overgrowth caused by expression of ptcJF03223 in clones in the wing disc.
    ykiB5/+ does not suppress the reduced eye size caused by WGMR.PG.
    One copy of ykiB5 suppresses the eye and head overgrowth phenotype seen in mopsfv11 Df(3L)H99 mutant clones (generated in a minute background).
    ykiB5/+ wings subjected to increased levels of cell death (by expression of debclScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4en-e16E) display a lower regenerative capacity than control wings.
    ykiB5/+ partially suppresses the retinal neurodegeneration seen in ft8 clones.
    A ykiB5 mutant background suppresses the overgrowth seen when tkvQ253D.Scer\UAS.cNc is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. As in ykiB5 mutants alone, very few clones are recoverable.
    The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr1 is suppressed by ykiB5/+.
    The small size of homozygous ykiB5 clones in the eye is rescued by expression of banA.Scer\UAS under the control of Scer\GAL4mirr-DE in the dorsal part of the eye.
    Expression of thV85M.Scer\UAS in wing disc clones (under the control of Scer\GAL4tub.PU) in ykiB5 mutant animals increases clone size compared to ykiB5 alone. Co-expression of dmScer\UAS.cZa in this background fails to rescue the growth defect of ykiB5 mutant clones, and growth rates are similar to when thV85M.Scer\UAS is expressed alone in ykiB5 mutant discs. Expression of banEP3622 in wing disc clones (under the control of Scer\GAL4tub.PU) in ykiB5 mutant animals increases clone size compared to ykiB5 alone. Co-expression of dmScer\UAS.cZa in this background fails to rescue the growth defect of ykiB5 mutant clones, and growth rates are similar to when banEP3622 is expressed alone in ykiB5 mutant discs. However, despite the small size of the clones, cell and nucleolar size are increased when dmScer\UAS.cZa and banEP3622 are expressed in ykiB5 wing discs. Expression of dmScer\UAS.cZa in ykiB5 wing disc clones (under the control of Scer\GAL4tub.PU) fails to rescue the growth defect of ykiB5 mutant clones. However, despite the small size of the clones, cell and nucleolar size are increased when dmScer\UAS.cZa is expressed in ykiB5 wing discs.
    The reduction in eye size caused by expression of sdScer\UAS.cSa under the control of Scer\GAL4GMR.PF is significantly enhanced by ykiB5/+. ykiB5/+ enhances the notched wing phenotype of sdETX4/Y males. Homozygous ykiB5 clones which are also expressing vgScer\UAS.cKa under the control of Scer\GAL4tub.PU are readily recovered in the wing blade.
    ft8/ft422 mutants, heterozygous for ykiB5 exhibit a size and morphology similar to those of wild-type discs, indicating suppression of the ft8/ft422 phenotype through ykiB5. ft8/ft422 mutants, heterozygous for ykiB5 progress to the late pupal stage of development, with some surviving to adulthood. ft8 ykiB5/ft422 flies display defects in the size and morphology of eyes, wings, and some bristles.
    ykiB5 mutant clones in the posterior of the wing disc that express banEP3622 under the control of Scer\GAL4hh-Gal4, grow to larger sizes than ykiB5 clones in the anterior of the disc. These clones are not as large as the wild-type twin spots.
    The growth-promoting effects of ft8 clones induced in the eye is strongly suppressed by one copy of ykiB5.
    Nearly all ft8 ykiB5/ftG-rv mutant animals develop into adults, although they do not hatch from the pupal case as they have some leg defects and head overgrowths. In contrast, all ft8/ftG-rv mutant animals die during the early stages of pupal development and show severely overgrown imaginal disc derivatives. This indicates that ykiB5 suppresses the overgrowth phenotypes of ft8/ftG-rv mutant imaginal discs.
    Xenogenetic Interactions
    Statement
    Reference
    Expression of the caspase inhibitor BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4Act5C.PP, in ykiB5 clones only weakly rescues the ykiB5-induced growth defects.
    Complementation and Rescue Data
    Comments
    Expression of ykiScer\UAS.cZb under the control of Scer\GAL4αTub84B.PL rescues the size of ykiB5 somatic clones. Expression of ykiΔ.Scer\UAS under the control of Scer\GAL4αTub84B.PL rescues the size of ykiB5 somatic clones.
    Expression of ykiScer\UAS.cZb under the control of Scer\GAL4GMR.PF rescues the growth defect seen in ykiB5 mutant clones. Expression of ykiS168A.Scer\UAS under the control of Scer\GAL4GMR.PF rescues the growth defect seen in ykiB5 mutant clones. Expression of ykiWW12.Scer\UAS under the control of Scer\GAL4GMR.PF fails to rescue the growth defect seen in ykiB5 mutant clones.
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    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    References (51)