UAS regulatory sequences drive expression of a XbaI-BglII/SfiI fragment and a BglII-SfiI fragment, both corresponding to the coding region of Sur, and cloned in opposite orientations to generate dsRNA with hairpin structure.
viable, with Scer\GAL4how-24B, Scer\GAL4twi.PG
viable, with Scer\GAL4Tub.PU
adult heart, with Scer\GAL4UAS.cHa, Scer\GAL4tin.cBa
1-week old progeny expressing SurdsRNA.Scer\UAS.4 under the simultaneous control of Scer\GAL4tin.cBa and Scer\GAL4Scer\UAS.cHa exhibit an increased rate of pacing-induced heart failure compared with controls.