Allele Dmel\Vha100-1³

General Information
Symbol	Dmel\Vha100-1³	Species	D. melanogaster
Name		FlyBase ID	FBal0194362
Feature type	allele	Associated gene	Dmel\Vha100-1

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Hiesinger et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Point mutation somewhere in exon 7, 3' to the point mutation in Vha100-1². (Hiesinger et al., 2005) Amino acid replacement: G?R. (Hiesinger et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage (with Df(3R)3450) (Hiesinger et al., 2005) lethal \| embryonic stage (with Vha100-1¹) (Hiesinger et al., 2005) lethal \| embryonic stage (with Vha100-1²) (Hiesinger et al., 2005) lethal \| embryonic stage (with Vha100-1⁴) (Hiesinger et al., 2005) lethal \| embryonic stage (with Vha100-1⁵) (Hiesinger et al., 2005) neurophysiology defective \| embryonic stage (with Vha100-1²) (Hiesinger et al., 2005) neurophysiology defective \| somatic clone (Hiesinger et al., 2005) phototaxis defective \| somatic clone (Hiesinger et al., 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies in which the retina is homozygous for Vha100-1³ (made using the 'ey-flp' system described in FBrf0125463) consistently fail to walk toward light in a phototaxis assay. Electro-retinograms from these animals display normal depolarization in response to light stimuli, but exhibit a complete loss of 'on' and 'off' transients, indicative of a failure to evoke a postsynaptic response. The frequency and amplitude of mini excitatory postsynaptic currents at the neuromuscular junctions of Vha100-1²/Vha100-1³ embryos is normal. However, excitatory postsynaptic currents induced by stimulation of the motor nerve supplying muscle 6 in these embryos have significantly lower amplitudes than in wild-type. As in wild-type, postsynaptic currents are induced in response to application of a hypertonic solution at the neuromuscular junction. (Hiesinger et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement Reference Vha100-1³, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is a non-suppressor \| somatic clone of eye phenotype of Syx1A^Scer\UAS.cBa/Syx1A^Scer\UAS.cBa (Hiesinger et al., 2005) Vha100-1³, Scer\GAL4^GMR.PF, Scer\GAL4^GMR.PF is a non-suppressor \| somatic clone of ommatidium phenotype of Syx1A^Scer\UAS.cBa/Syx1A^Scer\UAS.cBa (Hiesinger et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference The rough eye phenotype of Syx1A^Scer\UAS.cBa; Scer\GAL4^GMR.PF flies raised at 18^oC is not suppressed when the eye is made homozygous for Vha100-1³. (Hiesinger et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	v100³ (Hiesinger et al., 2005) Vha100-1³
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Hiesinger et al., 2005, Cell 121(4): 607--620 The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila. [FBrf0187227]

Allele Dmel\Vha100-13

Allele Dmel\Vha100-1³