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General Information
Symbol
Dmel\Vha100-13
Species
D. melanogaster
Name
FlyBase ID
FBal0194362
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
v1003
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G29135859C

Amino acid change:

G414R | Vha100-1-PA; G417R | Vha100-1-PB; G436R | Vha100-1-PC; G417R | Vha100-1-PD; G436R | Vha100-1-PE; G414R | Vha100-1-PF; G431R | Vha100-1-PG; G431R | Vha100-1-PI; G431R | Vha100-1-PJ; G417R | Vha100-1-PK

Reported amino acid change:

G?R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Site of amino acid change inferred from surrounding amino acid sequence.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G?R.

Point mutation somewhere in exon 7, 3' to the point mutation in Vha100-12.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Vha100-13 heterozygotes are viable.

Flies in which the retina is homozygous for Vha100-13 (made using the 'ey-flp' system described in FBrf0125463) consistently fail to walk toward light in a phototaxis assay. Electro-retinograms from these animals display normal depolarization in response to light stimuli, but exhibit a complete loss of 'on' and 'off' transients, indicative of a failure to evoke a postsynaptic response.

The frequency and amplitude of mini excitatory postsynaptic currents at the neuromuscular junctions of Vha100-12/Vha100-13 embryos is normal. However, excitatory postsynaptic currents induced by stimulation of the motor nerve supplying muscle 6 in these embryos have significantly lower amplitudes than in wild-type. As in wild-type, postsynaptic currents are induced in response to application of a hypertonic solution at the neuromuscular junction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Ubqn1 clones in the third instar larval salivary gland show aberrant morphology and accumulation of endoplasmic reticulum (sqh-EYFP-ER marker), which are not suppressed by Vha100-13 heterozygosity.

Ubqn1 clones in the third instar larval fat body show a severely decreased Lysotracker staining (marker of acidic lysosomes), which is significantly suppressed by Vha100-13 heterozygosity.

The rough eye phenotype of Syx1AScer\UAS.cBa; Scer\GAL4GMR.PF flies raised at 18oC is not suppressed when the eye is made homozygous for Vha100-13.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)