Open Close
General Information
Symbol
Scer\GAL4VGlut-OK371
Species
S. cerevisiae
Name
FlyBase ID
FBal0194519
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
OK371-GAL4, Vglut-Gal4, OK371, OK371Gal4, vGlutOK371-Gal4, GAL4-OK371, OK371 GAL4
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{GawB}VGlutOK371
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference

    Drives expression in glutamatergic neurons. Drives adult expression in neurites of mushroom body extrinsic neurons in the heel, and especially in the medial part of the beta and gamma lobes. Drives larval expression in a similar pattern, with additional expression around the peduncle and vertical lobe, as well as in the protocerebrum and ventral nerve cord.

    Positive GRASP (GFP reconstitution across synaptic partners) signals between Scer\GAL4VGlut-OK371 and adult Hugin neurons labelled with Ecol\lexAGMR61H10 were observed in the thoracic and abdominal ganglia.

    Expression of Scer\GAL4VGlut-OK371, which reflects expression of VGlut, within the protocerebrum, is seen within a dorso-laterally located group of cells, and where it colocalizes with sNPF protein. Expression of Scer\GAL4VGlut-OK371 in the nerve cord is extensive.

    In adults, Scer\GAL4VGlut-OK371 drives expression in neurites of mushroom body extrinsic neurons in the heel, and especially in the medial part of the beta and gamma lobes. Larval expression was similar to the one in adults, with additional expression around the peduncle and vertical lobe.

     
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Inhibition (using Hsap\KCNJ2FRT.Stop.UAS.EGFP) of Scer\GAL4VGlut-OK371 neurons expressing Scer\FLP1fru, in combination with a Gal80[ts] transgene to restrict expression to the 24 hours prior to testing and Scer\GAL80tsh-GAL80 to further restrict expression, in males, results in a significantly higher number courtship bouts of significantly shorter duration than controls, leading to significantly reduced levels of courtship behaviors overall (including wing extension and abdominal bending) in response to a female, with no difference in the rate of courtship initiation; copulation rate is also significantly reduced compared to controls.

    Thermogenetic activation (using TrpA1FRT.stop.UAS.mCherry at 31[o]C) of Scer\GAL4VGlut-OK371 neurons expressing Scer\FLP1fru in males does not affect courtship or copulation in response to females, compared to controls.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    NOT Enhanced by
    Statement
    Reference
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference
    Enhancer of
    NOT Enhancer of
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    NOT Enhanced by
    Statement
    Reference
    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference
    Enhancer of
    NOT Enhancer of
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Partially rescues
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (3)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Scer\GAL4OK371
    Scer\GAL4VGlut-OK371
    Name Synonyms
    Secondary FlyBase IDs
      References (192)