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General Information
Symbol
Dmel\5-HT1BdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0194589
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-1BRNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a 585bp cDNA fragment (nucleotides 571-1156) cloned in one orientation and then in the reverse orientation to generate a hairpin with a small intronic sequence in between.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of 5-HT1BIR.Scer\UAS under the control of Scer\GAL4tim.PE does not affect either the total amount of sleep or sleep consolidation, measured as the average length of sleep bouts.

Flies in which 5-HT1BIR.Scer\UAS is expressed under the control of Scer\GAL4tim.PE exhibit significantly increased light-induced phase shifts at low light intensity (250 lux) as compared to wild-type. In addition, 5-HTP treatment does not inhibit a strong light response in these flies.

Expression of 5-HT1BIR.Scer\UAS, under the control of Scer\GAL45-HT1B in flies that have been subjected to light pulses of different intensities results in significantly increased phase shifts as compared to wild-type flies. These flies are also immune to 5-HTP treatment.

Flies in which 5-HT1BIR.Scer\UAS is expressed under the control of Scer\GAL4tim.PE show significantly increased light-induced phase shifts at low light intensity as compared to control flies. In addition, 5-HTP treatment does not inhibit the light response of these flies.

Expression of 5-HT1BIR.Scer\UAS specifically in cells that normally express 5-HT1B (i.e. under the control of Scer\GAL45-HT1B) results in circadian periods significantly longer than those of controls. The same effect is observed when 5-HT1BIR.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of 5-HT1BIR.Scer\UAS, under the control of RU486-inducible Scer\GAL4Mef2.247.Switch in a 5-HT1AΔ5kb heterozygous background does not produce any baseline sleep phenotype.

Reducing 5-HT1B expression, through induction of 5-HT1BIR.Scer\UAS, under the control of Scer\GAL4tim.PE, eliminates the strong behavioural rhythms in constant bright light observed in cryb heterozygous flies overexpressing 5-HT1BScer\UAS.cYa under the control of Scer\GAL4tim.PE.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
5-HT1BIR.Scer\UAS
5-HT1BdsRNA.Scer\UAS
5-HT1BdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (8)