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General Information
Symbol
Scer\GAL4VGlut.PD
Species
S. cerevisiae
Name
Vesicular glutamate transporter promoter construct of Daniels
FlyBase ID
FBal0194614
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
VGlut-Gal4, DVGlut-GAL4, dvglutNMJX-Gal4, dvglutCNSIII-Gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

5.5kb fragment from immediately upstream of the VGlut translation start site is fused upstream of sequence encoding a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult mushroom body

Comment: faint expression

eye

Comment: medium expression

Additional Information
Statement
Reference

Drives expression in a subset of glutamatergic neurons in the larval and adult central nervous system.

Scer\GAL4VGlut.PD drives expression in the ellipsoid body rings in the innervation regions of R3 and R4d ring neurons and in a ring proximal to the R3 ring. In the fan-shaped body strong expression is detected in the central layers with weaker signal in the dorsal and ventral layers. Expression is also observed in the nodulus and in the protocerebral bridge (except for two segments on each lateral side). Co-localisation with mGluR protein is observed in the ellipsoid body rings R3 and R4d, in the fan-shaped body, nodulus and protocerebral bridge.

Scer\GAL4VGlut.PD is labels a subset of glutamatergic neurons in the medulla, lamina, lobula and lobula plate.

Expression of Scer\GAL4VGlut.PD is seen in many, but not all, glutamatergic neurons of the larva, and a few apparently non-glutamatergic neurons. In the adult brain, expression of VGlut shows extensive expression in central neuropil compartments, but is predominantly absent from the mushroom body only being detectable as punctae in the base of the gamma-lobes, the tip but not the base of the alpha-lobe (in contrast to VGlut which is seen in the base), the spur, and faintly in the calyx. Expression of Scer\GAL4VGlut.PD does not perfectly reflect expression of VGlut. Expression in the adult brain is seen in a few motor neurons that innervate head muscles. Expression within the ellipsoid body is observed within its inner and outer layers, a pattern suggestive of R-cell innervation. The expression seen in the protocerebral bridge is extensive across all glomeruli, but is weaker in the most distal glomerulus - glomerulus number 8. Expression in the adult optic lobe is strongest in an outer medulla layer and the inner medulla.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4VGlut.PD
Scer\GAL4VGlut
Name Synonyms
Vesicular glutamate transporter promoter construct of Daniels
Secondary FlyBase IDs
    References (34)