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General Information
Symbol
Dmel\Vps25K2
Species
D. melanogaster
Name
FlyBase ID
FBal0194621
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T8625577A

Reported nucleotide change:

T29A

Comment:

T to A mutation in the second base of the intron.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A transversion from T to A in the second base of the only Vps25 intron causes a splicing defect and, subsequently, premature termination of translation by an in-frame stop codon in the intron.

Nucleotide substitution: T29A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Vps25N55/Vps25K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type.

TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25K2 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25K2 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference

Vps25K2 is a suppressor of eye phenotype of hidGMR.PG/WGMR.PG

Additional Comments
Genetic Interactions
Statement
Reference

Vps25K2 moderate-strongly suppresses the WGMR.PG eye phenotype.

In Vps25N55/Vps25K2 mosaic eyes, in a WGMR.PG background, the rescued tissue is genetically wild-type or heterozygous, suggesting that Vps25N55 and Vps25K2 mutant tissue does not contribute to the suppression of WGMR.PG.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Vps25K2
su(GMR-hid)K2
Name Synonyms
Secondary FlyBase IDs
    References (1)