Vps25N55/Vps25K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type.
TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25K2 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25K2 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages.