Allele Dmel\Vps25^N55

General Information
Symbol	Dmel\Vps25^N55	Species	D. melanogaster
Name		FlyBase ID	FBal0194622
Feature type	allele	Associated gene	Dmel\Vps25
Also Known As	vps25^N55

Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Herz et al., 2006)
Mutagen	ethyl methanesulfonate (Herz et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Vps25^N55 carries a premature termination codon. (Herz et al., 2006) Amino acid replacement: Q93@. (Herz et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	cell growth defective \| cell non-autonomous \| somatic clone (Herz et al., 2006) hyperplasia \| cell non-autonomous \| somatic clone (Herz et al., 2009) increased cell death \| somatic clone (Herz et al., 2006) increased cell death \| somatic clone \| third instar larval stage (Herz et al., 2009) lethal (with Vps25^K2) (Herz et al., 2006)
Phenotype Manifest In
	adult head \| cell non-autonomous \| somatic clone (Herz et al., 2009) early endosome \| somatic clone (Herz et al., 2009) endosome \| somatic clone (Herz et al., 2006) eye \| cell non-autonomous \| somatic clone (Herz et al., 2009) eye \| somatic clone (with Vps25^K2) (Herz et al., 2006) eye-antennal disc \| cell autonomous \| somatic clone \| third instar larval stage (Herz et al., 2009) eye-antennal disc \| cell non-autonomous \| somatic clone \| third instar larval stage (Herz et al., 2009) eye disc \| somatic clone (Herz et al., 2006) wing disc \| cell non-autonomous \| somatic clone (Herz et al., 2006)
Detailed Description
	Statement Reference Vps25[N55] somatic clones in eye-antennal imaginal discs contain enlarged early endosomes. Vps25[N55] somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. Non-autonomous tissue overgrowth of the eye-antennal imaginal disc, adult eye and head occurs these animals. When the majority of cells in the eye-antennal imaginal discs are mutant for Vps25[N55], striking tissue overgrowth of the disc occurs and loose cellular architecture is observed. Vps25[N55] mutant somatic clones in eye-antennal imaginal discs do not proliferate very well, however the wildtype tissue immediately adjacent to the mutant clones show increased cell proliferation rates. (Herz et al., 2009) Vps25^N55/Vps25^K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type. TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25^N55 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25^N55 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages. Cell proliferation is significantly increased in tissue adjacent to Vps25^N55 clones. This non-autonomous cell proliferation is best visible in wing imaginal discs, where Vps25^N55 clones appear to be the origin for the increased proliferation of the adjacent tissue. Vps25^N55 eye-antennal disc mutant clones are unable to differentiate. Abnormal endosomal structures are found in Vps25^N55 mutant clones concurrent with increased ubiquitin and N-signaling activity. (Herz et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Vps25^N55 has cell growth defective \| somatic clone \| cell non-autonomous phenotype, non-enhanceable by N^DN.Scer\UAS/Scer\GAL4^tub.PU (Herz et al., 2006)
Suppressed by
Statement Reference Vps25^N55 has hyperplasia \| somatic clone \| cell non-autonomous phenotype, suppressible by N[+]/N^264-39 (Herz et al., 2009) Vps25^N55 has increased cell death \| somatic clone phenotype, suppressible by hpo^3D (Herz et al., 2006) Vps25^N55 has increased cell death \| somatic clone phenotype, suppressible by N^DN.Scer\UAS/Scer\GAL4^tub.PU (Herz et al., 2006) Vps25^N55 has increased cell death \| somatic clone phenotype, suppressible by Scer\GAL4^tub.PU/th^Scer\UAS.cHa (Herz et al., 2006)
Suppressor of
Statement Reference Vps25^N55 is a suppressor of increased cell death phenotype of W^GMR.PG/W^GMR.PG (Herz et al., 2006)
Other
Statement Reference Ark^H16, Vps25^N55 has cell growth defective \| somatic clone \| cell non-autonomous phenotype (Herz et al., 2006) Scer\GAL4^tub.PU, Vps25^N55, th^Scer\UAS.cHa has cell growth defective \| somatic clone \| cell non-autonomous phenotype (Herz et al., 2006)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Vps25^N55 has eye disc \| somatic clone phenotype, non-enhanceable by N^DN.Scer\UAS/Scer\GAL4^tub.PU (Herz et al., 2006)
Suppressed by
Statement Reference Vps25^N55 has adult head \| somatic clone phenotype, suppressible by N[+]/N^264-39 (Herz et al., 2009) Vps25^N55 has eye \| somatic clone phenotype, suppressible by N[+]/N^264-39 (Herz et al., 2009)
Suppressor of
Statement Reference Vps25^N55/Vps25^K2 is a suppressor of eye \| somatic clone \| cell non-autonomous phenotype of W^GMR.PHb (Herz et al., 2006) Vps25^N55 is a suppressor \| cell non-autonomous \| somatic clone of eye phenotype of W^GMR.PG/W^GMR.PG (Herz et al., 2009) Vps25^N55 is a suppressor of eye phenotype of W^GMR.PG/W^GMR.PG (Herz et al., 2006)
Other
Statement Reference Ark^H16, Scer\GAL4^tub.PU, Vps25^N55, puc^Scer\UAS.cMa has eye phenotype (Herz et al., 2006) Ark^H16, Vps25^N55 has eye phenotype (Herz et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for N[264-39] suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of Vps25[N55] generated in the eye-antennal disc. The eye-ablation phenotype in W[GMR.PG] animals is suppressed in Vps25[N55] somatic clones generated in the eye-antennal imaginal disc in a non-autonomous manner. (Herz et al., 2009) Vps25[N55] moderate-strongly suppresses the W[GMR.PG] eye phenotype. In Vps25[N55]/Vps25[K2] mosaic eyes, in a W[GMR.PG] background, the rescued tissue is genetically wild-type or heterozygous, suggesting that Vps25[N55] and Vps25[K2] mutant tissue does not contribute to the suppression of W[GMR.PG]. Vps25[N55]/N[DN.Scer\UAS] mutants (using the MARCM technique) exhibit reduced or absent Stat92E activity. Cell proliferation is not significantly increased in Vps25[N55]/N[DN.Scer\UAS] mutants, with eye imaginal discs appearing normal in shape and size. These Vps25[N55]/N[DN.Scer\UAS] double mutant clones exhibit the same level of apoptosis as Vps25[N55] single mutant clones. Blocking cell death, through expression of th[Scer\UAS.cHa] in Vps25[N55] mutant clones does not affect proliferation or Stat92E activity. Vps25[N55]/th[Scer\UAS.cHa] mosaics exhibit non-autonomous cell proliferation, as do Vps25[N55]/Ark[H16] mutants. Eye-antennal discs of Vps25[N55]/th[Scer\UAS.cHa] mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25[N55]/th[Scer\UAS.cHa] mosaics. Vps25[N55]/Ark[H16] cells undergo apoptosis. Vps25[N55]/Ark[H16] clones cannot be recovered in the adult eye. Vps25[N55]/th[Scer\UAS.cHa] and Vps25[N55]/Ark[H16] clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. The adult eye of Vps25[N55]/Ark[H16] mosaics is severely overgrown and folded. Thus, inhibiting cell death in Vps25[N55] clones can give rise to an even stronger overgrowth phenotype. Vps25[N55] Ark[H16]/puc[Scer\UAS.cMa] mosaic eye discs are severely overgrown. Vps25[N55] hpo[3D] mutant clones do not undergo apoptosis. (Herz et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Vps25^N55 is rescued by Vps25^hs.PH (Herz et al., 2006) Vps25^N55 is rescued by Vps25^Scer\UAS.cHa (Herz et al., 2006)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	N55 (Herz et al., 2006) su(GMR-hid)N55 (Herz et al., 2006) vps 25⁵⁵ (Childress et al., 2006) Vps25^N55 vps25^N55 (Herz et al., 2006, Herz et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Herz et al., 2009, PLoS ONE 4(1): e4165 Common and distinct genetic properties of ESCRT-II components in Drosophila. [FBrf0206508] Herz et al., 2006, Development 133(10): 1871--1880 vps25 mosaics display non-autonomous cell survival and overgrowth, and autonomous apoptosis. [FBrf0190294]
Supplementary material	Childress et al., 2006, Curr. Biol. 16(22): Supplemental Data. Lethal giant discs, a novel C2-domain protein, restricts Notch activation during endocytosis. [FBrf0202557]

Allele Dmel\Vps25N55

Allele Dmel\Vps25^N55