|Feature type||allele||Associated gene||Dmel\Vps25|
|Also Known As||vps25N55|
|Allele class||hypomorphic allele - genetic evidence|
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|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: Q93@.
|Phenotype Manifest In|
Vps25[N55] somatic clones in eye-antennal imaginal discs contain enlarged early endosomes. Vps25[N55] somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. Non-autonomous tissue overgrowth of the eye-antennal imaginal disc, adult eye and head occurs these animals. When the majority of cells in the eye-antennal imaginal discs are mutant for Vps25[N55], striking tissue overgrowth of the disc occurs and loose cellular architecture is observed. Vps25[N55] mutant somatic clones in eye-antennal imaginal discs do not proliferate very well, however the wildtype tissue immediately adjacent to the mutant clones show increased cell proliferation rates.
Vps25N55/Vps25K2 mosaic eyes are larger than wild-type, even if homozygous mutant clones are not recovered, indicating that the mutant clones do not contribute to the adult eye tissue, but appear to be able to induce overgrowth in wild-type tissue. Third-instar eye-antennal discs are overgrown and disorganised when compared with wild-type. TUNEL labelling, indicating the beginning of apoptosis, is increased in Vps25N55 mutant clones, consistent with the lack of mutant clones in the adult eye. Vps25N55 clones can grow to a fairly large size, suggesting that they are not growth-impaired. However, these clones are completely removed by apoptosis during pupal stages. Cell proliferation is significantly increased in tissue adjacent to Vps25N55 clones. This non-autonomous cell proliferation is best visible in wing imaginal discs, where Vps25N55 clones appear to be the origin for the increased proliferation of the adjacent tissue. Vps25N55 eye-antennal disc mutant clones are unable to differentiate. Abnormal endosomal structures are found in Vps25N55 mutant clones concurrent with increased ubiquitin and N-signaling activity.
|NOT Enhanced by|
Vps25N55 has hyperplasia | somatic clone | cell non-autonomous phenotype, suppressible by N[+]/N264-39
Vps25N55 has increased cell death | somatic clone phenotype, suppressible by NDN.Scer\UAS/Scer\GAL4tub.PU
|Phenotype Manifest In|
|NOT Enhanced by|
Heterozygosity for N[264-39] suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of Vps25[N55] generated in the eye-antennal disc. The eye-ablation phenotype in W[GMR.PG] animals is suppressed in Vps25[N55] somatic clones generated in the eye-antennal imaginal disc in a non-autonomous manner.
Vps25[N55] moderate-strongly suppresses the W[GMR.PG] eye phenotype. In Vps25[N55]/Vps25[K2] mosaic eyes, in a W[GMR.PG] background, the rescued tissue is genetically wild-type or heterozygous, suggesting that Vps25[N55] and Vps25[K2] mutant tissue does not contribute to the suppression of W[GMR.PG]. Vps25[N55]/N[DN.Scer\UAS] mutants (using the MARCM technique) exhibit reduced or absent Stat92E activity. Cell proliferation is not significantly increased in Vps25[N55]/N[DN.Scer\UAS] mutants, with eye imaginal discs appearing normal in shape and size. These Vps25[N55]/N[DN.Scer\UAS] double mutant clones exhibit the same level of apoptosis as Vps25[N55] single mutant clones. Blocking cell death, through expression of th[Scer\UAS.cHa] in Vps25[N55] mutant clones does not affect proliferation or Stat92E activity. Vps25[N55]/th[Scer\UAS.cHa] mosaics exhibit non-autonomous cell proliferation, as do Vps25[N55]/Ark[H16] mutants. Eye-antennal discs of Vps25[N55]/th[Scer\UAS.cHa] mosaics are extremely overgrown and can be 5-times as large as wild-type discs. Cell death is completely blocked in Vps25[N55]/th[Scer\UAS.cHa] mosaics. Vps25[N55]/Ark[H16] cells undergo apoptosis. Vps25[N55]/Ark[H16] clones cannot be recovered in the adult eye. Vps25[N55]/th[Scer\UAS.cHa] and Vps25[N55]/Ark[H16] clones occupy a large fraction of the eye discs, suggesting that these clones have no intrinsic growth disadvantage over wild-type tissue if cell death is blocked. The adult eye of Vps25[N55]/Ark[H16] mosaics is severely overgrown and folded. Thus, inhibiting cell death in Vps25[N55] clones can give rise to an even stronger overgrowth phenotype. Vps25[N55] Ark[H16]/puc[Scer\UAS.cMa] mosaic eye discs are severely overgrown. Vps25[N55] hpo[3D] mutant clones do not undergo apoptosis.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 5 )|
|Secondary FlyBase IDs|
|References ( 3 )|