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General Information
Symbol
Dmel\Camtates-2
Species
D. melanogaster
Name
termination slow2
FlyBase ID
FBal0194678
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A9471547C

Amino acid change:

S568R | Camta-PB; S405R | Camta-PD; S405R | Camta-PE; S568R | Camta-PF; S568R | Camta-PG; S200R | Camta-PH; S568R | Camta-PI

Reported amino acid change:

S577R

Comment:

Reported mutation locations are relative to GB:DQ902587. A nucleotide change at the first or third position of the Ser codon leads to an Arg mutation (base change unspecified).

Nucleotide change:

T9474419A

Amino acid change:

L1416term | Camta-PB; L1253term | Camta-PD; L1253term | Camta-PE; L1416term | Camta-PF; L1415term | Camta-PG; L1047term | Camta-PH; L1415term | Camta-PI

Reported amino acid change:

L1420term

Comment:

Reported mutation locations are relative to GB:DQ902587. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: S577R.

Amino acid replacement: L1420term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Electroretinogram (ERG) responses terminate slowly in Camtates-2 flies compared to wild-type flies. Whole-cell recordings of isolated Camtates-2 photoreceptor cells reveal a defective termination of light response.

The rhabdomeres of 1-day-old Camtates-2 flies show normal morphology.

Quantum bumps in wild-type and Camtates-2 photoreceptors have similar shapes and amplitude distributions. However, Camtates-2 mutant cells generate multiple bumps when stimulated with a flash of dim light, while wild-type cells produce only a single bump.

The minimum light intensity needed to induce prolonged depolarization afterpotentials is ~600 lux in Camtates-2 flies, while ~2600 lux is needed to induce this in wild-type flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Camtates-2 has abnormal neurophysiology phenotype, non-suppressible by ltlhs.PH

Enhancer of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Camtates-2 has eye photoreceptor cell phenotype, non-suppressible by ltlhs.PH

Camtates-2 has eye photoreceptor cell phenotype, non-suppressible by trpΔ1272

Enhancer of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The Gα49B1 mutation suppresses the multiple bump phenotype seen in Camtates-2 mutant photoreceptors following stimulation with a flash of dim light.

The trpΔ1272 mutation does not improve the termination response of Camtates-2 photoreceptors.

The termination photoresponses of Camtates-2; ninaE7 double mutant flies are as fast as wild-type flies, but the amplitude of the responses is decreased. In Camtates-2; ninaE7/+ flies, the response amplitude is similar to wild-type, but the termination response is increased compared to Camtates-2 single mutant flies.

The termination speed of photoresponses in Camtates-2; Arr25 double mutants is much slower than those of either single mutant.

Expression of either Fbxl4trp.PH or Fbxl4hs.PH rescues the slow-termination phenotype of Camtates-2 photoreceptors.

Expression of either CG4784hs.PH or CG32372hs.PH has no effect the Camtates-2 mutant phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by

Camtates-2 is partially rescued by CamtamlQ1.trp

Camtates-2 is partially rescued by CamtamlQ1.hs

Comments

Expression of Camtahs.PH, following a heat shock, results in a full rescue of the Camtates-2 phenotype. However, although high levels of Camta protein are seen at 1 hour following heat shock, full rescue is only achieved following 10 hours.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutation that affects phototransduction in an electroretinogram (ERG)-based screen.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Camtates-2
Name Synonyms
termination slow2
Secondary FlyBase IDs
    References (2)