Allele Dmel\Alk^{EC.Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Alk^{EC.Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0194692
Feature type	allele	Associated gene	Dmel\Alk

Allele class	antimorphic allele - genetic evidence
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Bazigou et al., 2007)
Mutagen	in vitro construct - coding region fusion (Bazigou et al., 2007) in vitro construct - regulatory fusion (Bazigou et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory regions drive expression of the extracellular and transmembrane domains of Alk, which is tagged at the C-terminus with a T:Hsap\MYC tag. (Bazigou et al., 2007)
Carried in construct	P{UAS-Alk.EC.MYC} (Bazigou et al., 2007, Bazigou et al., 2007, Gouzi et al., 2011)
Tagged with	T:Hsap\MYC (Bazigou et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell size \| adult stage, with Scer\GAL4^Ras64B.PW (Gouzi et al., 2011) learning defective \| adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) neuroanatomy defective \| somatic clone, with Scer\GAL4^Act5C.PI (Bazigou et al., 2007) size defective \| pupal stage, with Scer\GAL4^386Y (Gouzi et al., 2011) size defective \| pupal stage, with Scer\GAL4^Alk.38 (Gouzi et al., 2011) size defective \| pupal stage, with Scer\GAL4^elav-C155 (Gouzi et al., 2011) size defective \| pupal stage, with Scer\GAL4^Ras64B.PW (Gouzi et al., 2011) viable, with Scer\GAL4^elav-C155 (Gouzi et al., 2011)
Phenotype Manifest In
	axon & photoreceptor cell R8 \| somatic clone, with Scer\GAL4^Act5C.PI (Bazigou et al., 2007) visceral mesoderm, with Scer\GAL4^twi.PG (Bazigou et al., 2007) wing, with Scer\GAL4^Ras64B.PW (Gouzi et al., 2011)
Detailed Description
	Statement Reference Pan-neuronal expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[elav-C155], and restricted to the adult stages using Scer\GAL80[ts.αTub84B], causes a reproducible increase in associative olfactory learning performance compared to controls. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Ras64B.PW], and restricted to the adult stages using Scer\GAL80[ts.αTub84B], causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings, and a further enhancement in learning ability is seen with 6 pairings. Training with 12 CS/US pairings does not enhance the phenotype any further. The wings of the resulting adult flies are also larger than in wild type, due to increased cell size rather than cell number. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Alk.38], and restricted to the adult stages using Scer\GAL80[ts.αTub84B], causes a reproducible increase in associative olfactory learning performance compared to controls. This increase in performance is seen when flies are trained with 3 conditional stimulus (CS)/ unconditioned stimulus (US) pairings. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of either Scer\GAL4[Mef2.247] or Scer\GAL4[c747], and restricted to the adult stages using Scer\GAL80[ts.αTub84B], does not affect associative olfactory learning. Pan-neuronal expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[elav-C155] throughout development yields pupae that are 10-15% larger than controls. The resulting adults are viable and normally patterned. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Ras64B.PW] throughout development yields pupae that are 10-15% larger than controls. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Alk.38] throughout development yields pupae that are 10-15% larger than controls. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[386Y] throughout development yields pupae that are 10-15% larger than controls. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Ilp2.PR] throughout development yields pupae that are a similar size to controls. (Gouzi et al., 2011) The visceral mesoderm is disrupted in stage 14 embryos that express Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^twi.PG. R8 axons aberrantly terminate at the distal border of the medulla neuropil in animals that have clones overexpressing Alk^{EC.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act5C.PI in the visual system. The M3 recipient layer in these animals appear largely devoid of R8 terminals. (Bazigou et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^386Y is a suppressor \| partially of size defective \| recessive \| pupal stage phenotype of Nf1^E2 (Gouzi et al., 2011) Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Alk.38 is a suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011) Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ras64B.PW is a suppressor \| partially of size defective \| recessive \| pupal stage phenotype of Nf1^E2 (Gouzi et al., 2011) Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ras64B.PW is a suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011) Scer\GAL4^elav-C155/Alk^{EC.Scer\UAS.T:Hsap\MYC} is a suppressor \| partially of size defective \| recessive \| pupal stage phenotype of Nf1^E2 (Gouzi et al., 2011) Scer\GAL4^elav-C155/Alk^{EC.Scer\UAS.T:Hsap\MYC} is a suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011)
NOT Suppressor of
Statement Reference Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ccap.PP is a non-suppressor of size defective \| recessive \| larval stage phenotype of Nf1^E2 (Gouzi et al., 2011) Scer\GAL4^c747/Alk^{EC.Scer\UAS.T:Hsap\MYC} is a non-suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011) Scer\GAL4^ey-OK107/Alk^{EC.Scer\UAS.T:Hsap\MYC} is a non-suppressor of size defective \| recessive \| larval stage phenotype of Nf1^E2 (Gouzi et al., 2011)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[elav-C155] significantly suppresses the small pupae phenotype seen in Nf1[E2] homozygotes. The pupae seen are close to the size of controls. Rescue is also seen when Alk[EC.Scer\UAS.T:Hsap\MYC] is expressed under the control of Scer\GAL4[Ras64B.PW] or Scer\GAL4[386Y] but to a lesser extent. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[ey-OK107] does not suppress the small pupae phenotype seen in Nf1[E2] mutants. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Ccap.PP] does not suppress the small pupae phenotype seen in Nf1[E2] mutants. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of either the Scer\GAL4[elav-C155], Scer\GAL4[Ras64B.PW] or Scer\GAL4[Alk.38] driver, and restricted to the adult stages using Scer\GAL80[ts.αTub84B], rescues the olfactory learning defects seen in Nf1[E2]/Nf1[E1] mutants. Expression of Alk[EC.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[c747], and restricted to the adult stages using Scer\GAL80[ts.αTub84B], fails to rescue the olfactory learning defects seen in Nf1[E2]/Nf1[E1] mutants. (Gouzi et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Alk^{EC.Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Gouzi et al., 2011, PLoS Genet. 7(9): e1002281 The receptor tyrosine kinase alk controls neurofibromin functions in Drosophila growth and learning. [FBrf0216258] Bazigou et al., 2007, Cell 128(5): 961--975 Anterograde jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila. [FBrf0192674]
Supplementary material	Bazigou et al., 2007, Cell 128(5): Supplemental Data. Anterograde Jelly belly and Alk receptor tyrosine kinase signaling mediates retinal axon targeting in Drosophila. [FBrf0199314]

Allele Dmel\AlkEC.Scer\UAS.T:Hsap\MYC

Allele Dmel\Alk^{EC.Scer\UAS.T:Hsap\MYC}